Using DeepLabCut for markerless cardiac physiology and toxicity estimation in water fleas (Daphnia magna)

Saputra, Ferry; Suryanto, Michael Edbert; Audira, Gilbert; Luong, Cao Thang; Hung, Chih-Hsin; Roldan, Marri Jmelou; Vasquez, Ross D.; Hsiao, Chung-Der

doi:10.1016/j.aquatox.2023.106676

Aquatic Toxicology

2023

DOI: 10.1016/j.aquatox.2023.106676

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Using DeepLabCut for markerless cardiac physiology and toxicity estimation in water fleas (Daphnia magna)

Ferry Saputra,

Michael Edbert Suryanto,

Gilbert Audira

et al.

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2024

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Cited by 3 publications

References 39 publications

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Automated counting and classifying Daphnia magna using machine vision

Ma,

Xiao,

Wang

et al. 2024

Aquatic Toxicology

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Automated counting and classifying Daphnia magna using machine vision

Ma,

Xiao,

Wang

et al. 2024

Aquatic Toxicology

View full text Add to dashboard Cite

Water acidification aggravates lithium-induced toxicity represented by energy supply, oxidative stress, and cell fate in Daphnia magna neonates

Zhao,

Duan,

Xiao

et al. 2024

Science of The Total Environment

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Determining the Response of Water Fleas to Some of the Excitatory and Toxic Chemical Solutions by Heart Rates

Karademir,

Can,

Sönmez

et al. 2024

Turkish Journal of Maritime and Marine Sciences

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Water fleas have become a valuable model for ecotoxicity studies due to their ease of cultivation, transparent bodies, and high sensitivity to chemical pollutants. In this study, heart rates in Daphnia magna were examined reflecting the basic behavioral and physiological characteristics when exposed to known toxic chemicals. Crystal violet, ethanol, and formaldehyde, known for their toxicity, were selected due to their different chemical properties. A slow-motion video-based method was organized to measure cardiovascular performance by evaluating the heart rates in water fleas. Data for important parameters like heart rates, body reactions, and heart contraction were extracted from video recordings and mathematical calculations. The results of study were quite remarkable. It was found that crystal violet increased the heart rates of Daphnia magna (489±14.19) more significantly than ethanol (450±40.67) and formaldehyde (445±48.21). Compared to the control group, formaldehyde caused a 28.51% increase in the heart rates of daphnids, while exposure to ethanol (30.54%) and crystal violet (35.89%) resulted in a lower increase in the heart rates of daphnids. Using these cardiovascular parameters, the potential effects of excitatory and toxic chemical solutions on water fleas were accurately measured. Daphnia magna was identified as having the strongest heart rates and is deemed suitable for ecotoxicity assessment. The result of exposure to all three chemicals was an increase in the organism’s heart rates during short-term exposures, while long-term exposures could lead to toxic effects, causing fatalities.

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Using DeepLabCut for markerless cardiac physiology and toxicity estimation in water fleas (Daphnia magna)

Cited by 3 publications

References 39 publications

Automated counting and classifying Daphnia magna using machine vision

Automated counting and classifying Daphnia magna using machine vision

Water acidification aggravates lithium-induced toxicity represented by energy supply, oxidative stress, and cell fate in Daphnia magna neonates

Determining the Response of Water Fleas to Some of the Excitatory and Toxic Chemical Solutions by Heart Rates

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