Using DNA Barcoding for Fingerprinting of Two Important Forage Crops Varieties (Alfalfa And Egyptian Clover)

Abstract

Study was executed to differentiate and discriminate (Medicago sativa and Trifolium alexandrinum) using DNA barcoding genes [rbcl] and Cox1 genes. Identification of (Medicago sativa (Rammah 1) was completed via rbcl and Cox1 genes and was identified as Medicago sativa voucher G00199095 ribulose1,5 bisphosphate carboxylase / oxygenase large subunit gene, partial cds; chloroplast Sequence ID: KJ204375.1 or Medicago sativa voucher Ahrendsen 23 for rbcl and Cox1 genes. Identity estimation were listed with 90% as alfalfa, Rammah 1 Genotype ribulose1 /5 bisphosphate carboxylase / oxygenase large subunit gene sequences ID: KJ206375.1] also, identity values of 91.24% were recorded with for alfalfa Rammah 1 Genotype, cytochrome c oxidase subunit I gene cox 1 sequences ID: KJ 204375.1). Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) by rbcl and Cox1 genes respectively. Affiliation of genetic source was revealed for Trifolium alexandrinum with 100 % match with origin which indicate rising possibility for applying discrimination through comparing with Medicago sativa which reflect the lowest genetic likeness with the source. Moreover, we might detect from the available data that we can use DNA Bar-coding Technique in Discriminating the local Egyptian Clover Genotypes and Protect Them internationally. Also, DNA Bar-coding can be used to determine the genetic polymorphism in identifying superior genotypes as source of parental genotypes in Egyptian clover breeding program in future.

Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM

“…SCoT uses a single 18-mer primer in a polymerase chain reaction (PCR) assays and requires an annealing temperature of 50 °C [ 14 ]. The ISSR and SCoT polymorphism has been used in genetic resource differentiation, cultivar characterization, and marker-assisted breeding programs in many plants; examples include alfalfa and Egyptian clover [ 15 ], Nigella sativa [ 16 ], Medicago sativa [ 17 ], Pistacia lentiscus [ 18 ], Moringa oleifera [ 19 ], Lathyrus species [ 20 ], Crepidium acuminatum [ 21 ], maize [ 22 ], Hordeum [ 23 ], and smooth bromegrass [ 24 , 25 ].…”

“…Recently, DNA barcoding has become a valuable technique for assessing biodiversity in phylogenetic reconstruction and plant evolution [ 37 – 39 ]. Combined nuclear and chloroplast DNA sequences were used to barcode the major forage plants [ 15 , 18 , 40 ]. Knowledge about the genetic diversity of 12 bread wheat cultivars is limited, not only among Northern African varieties but also in cultivars in different countries.…”

Genetic Diversity among Selected Medicago sativa Cultivars Using Inter-Retrotransposon-Amplified Polymorphism, Chloroplast DNA Barcodes and Morpho-Agronomic Trait Analyses

“…Combined universal chloroplast and nuclear DNA sequence targets was applied to barcode the major Mediterranean leguminous crops [ 31 ]. The combination of universal chloroplast and nuclear DNA sequence targets was applied to barcode the major Mediterranean leguminous crops [ 32 ].…”