Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases

Cutlan, Rhys; Rose, S.A. De; Isupov, Michail N.; Littlechild, Jennifer A.; Harmer, Nicholas J.

doi:10.1016/j.bbapap.2019.140322

Cited by 35 publications

(33 citation statements)

References 106 publications

(131 reference statements)

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“…In this work the issues of cross reactivity and reaction incompatibility in linear enzymatic cascades are addressed whilst also showing compartmentalized flow systems can be used to overcome metabolic flux issues associated with in vitro cascades. 17 This allows the expansion of the scope of biocatalytic cascades available through exploitation of previously incompatible systems ( Figure 1). The aim was to adopt a modular approach using standard laboratory equipment to generate versatile systems which enable access to a broad range of chemistries in a sustainable manner.…”

Section: Mainmentioning

confidence: 99%

Production of High Value Amine Intermediates via Biocatalytic Cascades in Continuous Flow

Mattey¹,

ford²,

Citoler³

et al. 2021

Preprint

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<div> <p>A key aim of biocatalysis is to mimic the ability of eukaryotic cells to carry out compartmentalized multistep cascades in a controlled and selective way. As biocatalytic cascades get longer and more complex, reactions become unattainable under typical batch conditions. Here a continuous flow multipoint injection reactor was combined with switching valves to overcome batch incompatibility, thus allowing for successful biocatalytic reaction cascades. As proof-of-principle, several reactive carbonyl intermediates were generated <i>in situ </i>using galactose oxidase and engineered choline oxidases, then passed directly to a series of packed-bed modules containing different aminating biocatalysts which accordingly produced a range of structurally distinct amines. The method was expanded to employ a batch incompatible sequential amination cascade <i>via </i>an oxidase-transaminase-imine reductase sequence, introducing different amine reagents at each step without cross reactivity. The combined approaches allowed for the biocatalytic synthesis of the natural product alkaloid precursor 4O-methylnorbelladine. The flow biocatalysis platform shown here significantly increases the scope of novel biocatalytic cascades, removing previous limitations due to reaction and reagent batch incompatibility.</p> </div> <b><br></b>

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Section: Mainmentioning

confidence: 99%

Production of High Value Amine Intermediates via Biocatalytic Cascades in Continuous Flow

Mattey¹,

ford²,

Citoler³

et al. 2021

Preprint

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“…These advances have accelerated the arrival of the "Fourth Wave of Biocatalysis" (Bornscheuer, 2018) or the so-called "Golden Age of Biocatalysis" (Devine et al, 2018); combinations of enzymes -whether in cascade reactions or via metabolic engineering-brings together many beneficial features which might convert this strategy as the 'first choice' to advance in the biotransformation field (Bornscheuer, 2018). The huge research efforts in this field are reflected by different reviews from the last decade on general operational and functional aspects on different MECs, some of them also including disseminated information on AA production using chemoenzymatic and multienzymatic systems (Lopez-Gallego and Schmidt-Dannert, 2010;Hall and Bommarius, 2011;Ricca et al, 2011;Oroz-Guinea and García-Junceda, 2013;France et al, 2017;Quin et al, 2017;Devine et al, 2018;Schrittwieser et al, 2018;Sperl and Sieber, 2018;Wu and Li, 2018;Cutlan et al, 2019;Hwang and Lee, 2019;Giannakopoulou et al, 2020). The interest on MECs in biotransformation and biomedical engineering is thus clear, being an attractive alternative for the production of biofuels, pharmaceuticals and fine chemicals (Hwang and Lee, 2019).…”

Section: Multienzymatic Cascades For the Production Of Ncaasmentioning

confidence: 99%

“…TAs are well documented enzymes for the synthesis of NcAAs ( Meiwes et al, 1997 ; Taylor et al, 1998 ; Li et al, 2002 ; Rozzell and Bommarius, 2002 ), but their biotechnological interest is far beyond the production of these compounds, since they allow the production of other important molecules [e.g., β-AAs ( Rudat et al, 2012 ) or amines ( Höhne and Bornscheuer, 2009 )]. TAs continue receiving huge attention, and have been reviewed extensively during the last decade due to their huge biotechnological interest (e.g., Mathew and Yun, 2012 ; Simon et al, 2014 ; Guo and Berglund, 2017 ; Slabu et al, 2017 ; Patil et al, 2018 ; Xue et al, 2018 ; Cutlan et al, 2019 ). Protein engineering strategies have been broadly used to evolve ω-TAs (e.g., Park et al, 2013a , b ; Walton et al, 2017 ), and a database on sequences and structures of biotechnologically relevant engineered ω-TAs is available ( Buß et al, 2018 ).…”

Section: Multienzymatic Cascades For the Production Of Ncaasmentioning

confidence: 99%

“…The nomenclature of TAs in the literature can be confusing. We recommend reading of the new classification carried out byCutlan et al (2019).Frontiers in Bioengineering and Biotechnology | www.frontiersin.org…”

mentioning

confidence: 99%

“… 5 The nomenclature of TAs in the literature can be confusing. We recommend reading of the new classification carried out by Cutlan et al (2019) . …”

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confidence: 99%

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Overview on Multienzymatic Cascades for the Production of Non-canonical α-Amino Acids

Martínez‐Rodríguez

Torres

Sánchez

et al. 2020

Front. Bioeng. Biotechnol.

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The 22 genetically encoded amino acids (AAs) present in proteins (the 20 standard AAs together with selenocysteine and pyrrolysine), are commonly referred as proteinogenic AAs in the literature due to their appearance in ribosome-synthetized polypeptides. Beyond the borders of this key set of compounds, the rest of AAs are generally named imprecisely as non-proteinogenic AAs, even when they can also appear in polypeptide chains as a result of post-transductional machinery. Besides their importance as metabolites in life, many of D-α-and L-α-"non-canonical" amino acids (NcAAs) are of interest in the biotechnological and biomedical fields. They have found numerous applications in the discovery of new medicines and antibiotics, drug synthesis, cosmetic, and nutritional compounds, or in the improvement of protein and peptide pharmaceuticals. In addition to the numerous studies dealing with the asymmetric synthesis of NcAAs, many different enzymatic pathways have been reported in the literature allowing for the biosynthesis of NcAAs. Due to the huge heterogeneity of this group of molecules, this review is devoted to provide an overview on different established multienzymatic cascades for the production of non-canonical D-α-and L-α-AAs, supplying neophyte and experienced professionals in this field with different illustrative examples in the literature. Whereas the discovery of new or newly designed enzymes is of great interest, dusting off previous enzymatic methodologies by a "back and to the future" strategy might accelerate the implementation of new or improved multienzymatic cascades.

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Multienzymatic Cascades and Nanomaterial Scaffolding—A Potential Way Forward for the Efficient Biosynthesis of Novel Chemical Products

Hooe,

Smith,

Dean

et al. 2023

Advanced Materials

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Synthetic biology is touted as the next industrial revolution as it promises access to greener biocatalytic syntheses to replace many industrial organic chemistries. Here, it is shown to what synthetic biology can offer in the form of multienzyme cascades for the synthesis of the most basic of new materials—chemicals, including especially designer chemical products and their analogs. Since achieving this is predicated on dramatically expanding the chemical space that enzymes access, such chemistry will probably be undertaken in cell‐free or minimalist formats to overcome the inherent toxicity of non‐natural substrates to living cells. Laying out relevant aspects that need to be considered in the design of multi‐enzymatic cascades for these purposes is begun. Representative multienzymatic cascades are critically reviewed, which have been specifically developed for the synthesis of compounds that have either been made only by traditional organic synthesis along with those cascades utilized for novel compound syntheses. Lastly, an overview of strategies that look toward exploiting bio/nanomaterials for accessing channeling and other nanoscale materials phenomena in vitro to direct novel enzymatic biosynthesis and improve catalytic efficiency is provided. Finally, a perspective on what is needed for this field to develop in the short and long term is presented.

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Using enzyme cascades in biocatalysis: Highlight on transaminases and carboxylic acid reductases

Cited by 35 publications

References 106 publications

Production of High Value Amine Intermediates via Biocatalytic Cascades in Continuous Flow

Production of High Value Amine Intermediates via Biocatalytic Cascades in Continuous Flow

Overview on Multienzymatic Cascades for the Production of Non-canonical α-Amino Acids

Multienzymatic Cascades and Nanomaterial Scaffolding—A Potential Way Forward for the Efficient Biosynthesis of Novel Chemical Products

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