Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line

Malaiyandi, Latha M.; Sharthiya, Harsh; Barakat, Ameir; Edwards, Joshua R.; Dineley, Kirk E.

doi:10.1007/s10534-019-00226-z

Cited by 3 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is possible to use commercially available fluorprobes to detect intracellular cadmium. We recently reported on using divalent fluorophores to detect entry of Cd into MIN6 cells [ 45 ]. However, there are limitations as currently available Cd flurophores exhibit significant cross reactivity to other divalent metals.…”

Section: Discussionmentioning

confidence: 99%

Pancreatic Islets Accumulate Cadmium in a Rodent Model of Cadmium-Induced Hyperglycemia

Fitzgerald

Olsen

Nguyen

et al. 2020

IJMS

View full text Add to dashboard Cite

Cadmium (Cd) is an anthropogenic as well as a naturally occurring toxicant associated with prediabetes and T2DM in humans and experimental models of Cd exposure. However, relatively few studies have examined the mechanism(s) of Cd-induced hyperglycemia. The purpose of this study was to examine the role of pancreatic islets in Cd-induced hyperglycemia. Male Sprague–Dawley rats were given daily subcutaneous doses of Cd at 0.6 mg/kg over 12 weeks. There was a resulting time-dependent increase in fasting blood glucose and altered insulin release in vitro. Islets isolated from control (saline-treated) and Cd-treated animals were incubated in low (0.5 mg/mL) or high (3 mg/mL) glucose conditions. Islets from 12 week Cd-treated animals had significantly less glucose-stimulated insulin release compared to islets from saline-treated control animals. The actual Cd content of isolated islets was 5 fold higher than the whole pancreas (endocrine + exocrine) and roughly 70% of that present in the renal cortex. Interestingly, islets isolated from Cd-treated animals and incubated in high glucose conditions contained significantly less Cd and zinc than those incubated in low glucose. These results show that within whole pancreatic tissue, Cd selectively accumulates in pancreatic islets and causes altered islet function that likely contributes to dysglycemia.

show abstract

Section: Discussionmentioning

confidence: 99%

Pancreatic Islets Accumulate Cadmium in a Rodent Model of Cadmium-Induced Hyperglycemia

Fitzgerald

Olsen

Nguyen

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Assuming only the formation of a monocomplex and excluding formation of the ternary complex formation, a pH-independent stability constant of logβ = 7.44 ± 0.01 was determined by evaluating the titration curves with the PSEQUAD program [58]. Recalculating this value at pH = 7.0, the obtained value of 7.18 was close to the available literature data for the Cd(II)-FluoZin-3 monocomplex (logβ = 6.9 [59]) (Figure S3a).…”

Section: Interaction Of 1mey# Zinc Finger Protein With Cd(ii)mentioning

confidence: 57%

Interactions of an Artificial Zinc Finger Protein with Cd(II) and Hg(II): Competition and Metal and DNA Binding

2023

View full text Add to dashboard Cite

Cys2His2 zinc finger proteins are important for living organisms, as they—among other functions—specifically recognise DNA when Zn(II) is coordinated to the proteins, stabilising their ββα secondary structure. Therefore, competition with other metal ions may alter their original function. Toxic metal ions such as Cd(II) or Hg(II) might be especially dangerous because of their similar chemical properties to Zn(II). Most competition studies carried out so far have involved small zinc finger peptides. Therefore, we have investigated the interactions of toxic metal ions with a zinc finger proteins consisting of three finger units and the consequences on the DNA binding properties of the protein. Binding of one Cd(II) per finger subunit of the protein was shown by circular dichroism spectroscopy, fluorimetry and electrospray ionisation mass spectrometry. Cd(II) stabilised a similar secondary structure to that of the Zn(II)-bound protein but with a slightly lower affinity. In contrast, Hg(II) could displace Zn(II) quantitatively (logβ′ ≥ 16.7), demolishing the secondary structure, and further Hg(II) binding was also observed. Based on electrophoretic gel mobility shift assays, the Cd(II)-bound zinc finger protein could recognise the specific DNA target sequence similarly to the Zn(II)-loaded form but with a ~0.6 log units lower stability constant, while Hg(II) could destroy DNA binding completely.

show abstract

Cadmium inhibits calcium activity in hippocampal CA1 neurons of freely moving mice

Matsushita,

Xia

2024

Toxicological Sciences

View full text Add to dashboard Cite

Cadmium (Cd) is a ubiquitous toxic heavy metal and a potential neurotoxicant due to its wide use in industrial manufacturing processes and commercial products, including fertilizers. The general population is exposed to Cd through food and smoking due to high transfer rates of Cd from contaminated soil. Cd has been shown to mimic calcium ions (Ca2+) and interfere with intracellular Ca2+ levels and Ca2+ signaling in in vitro studies. However, nothing is known about Cd’s effects on Ca2+ activity in neurons in live animals. This study aimed to determine if Cd disrupts Ca2+ transients of neurons in CA1 region of the hippocampus during an associative learning paradigm. We utilized in vivo Ca2+ imaging in awake, freely moving C57BL/6 mice to measure Ca2+ activity in CA1 excitatory neurons expressing genetically encoded Ca2+ sensor GCaMP6 during an associative learning paradigm. We found that a smaller proportion of neurons are activated in Cd-treated groups compared to control during fear conditioning, suggesting that Cd may contribute to learning and memory deficit by reducing activity of neurons. We observed these effects at Cd exposure levels that result in blood Cd levels comparable to the general US population levels. This provides a possible molecular mechanism for Cd interference of learning and memory at exposure levels relevant to US adults. To our knowledge, our study is the first to describe Cd effects on brain Ca2+ activity in vivo in freely behaving mice.

show abstract

Using FluoZin-3 and fura-2 to monitor acute accumulation of free intracellular Cd2+ in a pancreatic beta cell line

Cited by 3 publications

References 40 publications

Pancreatic Islets Accumulate Cadmium in a Rodent Model of Cadmium-Induced Hyperglycemia

Pancreatic Islets Accumulate Cadmium in a Rodent Model of Cadmium-Induced Hyperglycemia

Interactions of an Artificial Zinc Finger Protein with Cd(II) and Hg(II): Competition and Metal and DNA Binding

Cadmium inhibits calcium activity in hippocampal CA1 neurons of freely moving mice

Contact Info

Product

Resources

About