Using fungi and yeasts to manage vegetable crop diseases

Punja, Zamir K.; Utkhede, R. S.

doi:10.1016/s0167-7799(03)00193-8

Cited by 188 publications

(131 citation statements)

References 71 publications

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“…Biocontrol agents can be effective through the production of hydrolytic enzymes and antibiotics, niche colonisation and competition for host nutrients, induction of plant host defence mechanisms, and interference with pathogenicity factors (Punja and Utkhede 2003;Arzanlou et al 2014). Species of Bacillus, Pseudomonas, and Paenibacillus have an inhibitory effect on a wide range of phytopathogenic fungi, using diverse ranges of mechanisms including the production of hydrolytic enzymes and antibiotics, niche colonisation and competition for host nutrients, induction of plant host defence systems, and interference with pathogenicity factors McSpadden Gardener 2004;Ongena et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

Arzanlou¹,

Mousavi²,

Bakhshi³

et al. 2016

Journal of Plant Protection Research

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Abstract:In the present study, the antagonistic capability of bacterial agents inhabiting the rhizosphere of sugarbeet plants were evaluated against Cercospora beticola Sacc. under laboratory and greenhouse conditions. After preliminary screening using the dual culture method, 14 strains with higher antagonistic capability were selected for further inhibitory assays against C. beticola. Bacterial strains were identified based on the sequence data of the small subunit-rDNA (SSU-rDNA) gene. Based on the SSU sequence data, the identity of bacterial strains were determined as Bacillus (10 strains: RB1, RB2, RB3, RB4, RB5, RB6, RB7, RB8, RB9, RB10), Paenibacillus (two strains: RP1, RP2), Enterobacter (one strain: RE), and Pseudomonas (one strain: RPs). The results obtained in this study showed that in all of the assays (dual culture, volatile and non-volatile metabolites) bacterial antagonists significantly inhibited the growth of C. beticola compared to the control. Bacillus (RB2) showed the highest inhibition rate on C. beticola in all of the assays. Based on the results of the laboratory assays, three bacterial strains RB2 (Bacillus), RPs (Pseudomonas), and RE (Paenibacillus) were selected for greenhouse assays. The experiment was designed based on a completely randomised design (CRD) with the application of antagonists prior to, simultaneously, and after inoculation with C. beticola on sugarbeet leaves. The reduction in disease severity was evaluated seven days after inoculation. The results of greenhouse assays were consistent with the results of laboratory studies. The obtained results showed that bacterial antagonists significantly reduced the disease severity when compared to the control.

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Section: Introductionmentioning

confidence: 99%

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

Arzanlou¹,

Mousavi²,

Bakhshi³

et al. 2016

Journal of Plant Protection Research

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“…A number of bacteria, fungi and yeasts have been reported to have potential for use as biocontrol agents (BCAs) in vegetables (Punja 1997), and some are commercially available (Punja and Utkhede 2003). In general seed treatment is regarded as an efficient method of delivery of microbes.…”

Section: Introductionmentioning

confidence: 99%

Control of seed-borne pathogens on legumes by microbial and other alternative seed treatments

et al. 2008

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“…Long-term storage of this important fruit has become possible due to modern postharvest technologies. However, postharvest diseases, especially those originating from wound infection at harvest or during postharvest handling and packing, can be a serious limiting factor for management alternatives with different mechanisms of action compared to chemical fungicides (Punja and Utkhede, 2003;Spadaro and Gullino, 2004;Fravel, 2005;Massart and Jijakli, 2007). A number of yeasts and bacteria have been reported to control postharvest fruit decay effectively (Janisiewicz and Korsten, 2002).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun

Bajji

Massart

et al. 2007

Postharvest Biology and Technology

128

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Aureobasidium pullulans strain Ach1-1 was selected for its effectiveness against blue mold caused by Penicillium expansum on stored apple fruit. The possible involvement of competition for nutrients in the biocontrol activity of this antagonistic strain was investigated both in vitro and in situ. For in vitro assays, the effect of strain Ach1-1 on germination percentages of P. expansum conidia was evaluated after a 24 h incubation period in the presence of increasing apple juice concentrations (0-5%) using a system allowing the physical separation of both agents. In the absence of strain Ach1-1, conidial germination was strongly promoted by apple juice whatever the concentration. However, germination was significantly reduced by the presence of strain Ach1-1 except at the highest juice concentration. For conidia previously inhibited at 0.5% juice, germination after 24 h of incubation was partially recovered in the presence of strain Ach1-1 when fresh juice was added to a final concentration of 5%, and completely restored at both 0.5 and 5% juice concentrations in the absence of strain Ach1-1. For in situ assays, strain Ach1-1 was very protective against P. expansum on postharvest wounded apples. However, the application of high concentrations of exogenous sugars, vitamins and most particularly amino acids, significantly reduced such protection. Time-course analysis of apple amino acids at the wound site revealed that these compounds were more depleted in wounds treated with strain Ach1-1 alone and especially in those treated with both agents (strain Ach1-1 and P. expansum) compared to wounds treated with P. expansum alone or to untreated ones. Exogenous amino acids, applied at high concentrations on apple wounds as a mixture of specific amino acid groups or as individuals, significantly decreased strain Ach1-1 efficacy against P. expansum. The present study provides in vitro and in situ evidence that competition for apple nutrients, most particularly amino acids, may be a main mechanism of the biocontrol activity of A. pullulans strain Ach1-1 against blue mold caused by P. expansum on harvested apple fruit.

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Using fungi and yeasts to manage vegetable crop diseases

Cited by 188 publications

References 71 publications

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet

Control of seed-borne pathogens on legumes by microbial and other alternative seed treatments

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

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