Using gadolinium ions as affinity probes to selectively enrich and magnetically isolate bacteria from complex samples

Wei, Jialin; Huang, De-Yi; Chen, Yu-Chie

doi:10.1016/j.aca.2020.03.046

Cited by 13 publications

(17 citation statements)

References 39 publications

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“…The affinity separation and subsequent terminal differentiation of acute myeloid leukemia cells were achieved using the human transferrin receptor (CD71) as a capture target [155]. The cell affinity sorbents for Human Immunodeficiency Virus-1 virus-like particles, tandem single-chain variable fragment bispecific antibodies, and various types of bacteria were also developed by different researchers [156][157][158][159][160]. Biodegradable anisotropic microparticles, earthicle-based ferrofluids (e.g.…”

Section: Cell Affinity Capturementioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Section: Cell Affinity Capturementioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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“…Thus, there is an immense need for rapid and cost-effective techniques that concentrate target bacteria while effectively removing them from the interfering food matrix. Rapid detection methods such as biosensors and PCR often employ techniques such as centrifugation [ 16 , 17 ], filtration [ 17 ], dielectrophoresis [ 18 ], metal hydroxides [ 19 , 20 ], and magnetic nanoparticles (MNPs) [ 21 , 22 , 23 , 24 ] to attempt to fulfill these needs without overnight culturing ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…Both methods lack specificity and cannot target specific bacteria species [ 26 , 30 ]. Similarly, metal hydroxides are non-specific and often have high limits of detection, or the assays require incubation before subsequent pathogen detection [ 19 , 20 , 30 , 31 ]. Some chemical methods allow for specificity and debris removal, such as dielectrophoresis [ 18 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Dester

Alocilja

2022

Biosensors

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Rapid and accurate food pathogen detection is an essential step to preventing foodborne illnesses. Before detection, removal of bacteria from the food matrix and concentration to detectable levels are often essential steps. Although many reviews discuss rapid concentration methods for foodborne pathogens, the use of glycan-coated magnetic nanoparticles (MNPs) is often omitted. This review seeks to analyze the potential of this technique as a rapid and cost-effective solution for concentration of bacteria directly from foods. The primary focus is the mechanism of glycan-coated MNP binding, as well as its current applications in concentration of foodborne pathogens. First, a background on the synthesis, properties, and applications of MNPs is provided. Second, synthesis of glycan-coated particles and their theorized mechanism for bacterial adhesion is described. Existing research into extraction of bacteria directly from food matrices is also analyzed. Finally, glycan-coated MNPs are compared to the magnetic separation technique of immunomagnetic separation (IMS) in terms of cost, time, and other factors. At its current state, glycan-coated MNPs require more research to fully identify the mechanism, potential for optimization, and extraction capabilities directly in food matrices. However, current research indicates glycan-coated MNPs are an incredibly cost-effective method for rapid food pathogen extraction and concentration.

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“…We previously have demonstrated that nonmagnetic species, such as bacterial and cells, can gain apparent magnetism by simply mixing with magnetic metal ions, such as Fe 3+24 and Gd 3+ . 25 The binding mechanism is based on the hard−soft acid−base (HSAB) theory. 26 Hard acids such as Fe 3+ have good affinity to bind with hard bases such as carboxylates.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Magnetic isolation can be easily conducted and only a small magnet is sufficient for conducting magnetic isolation of the conjugates of magnetic nanoprobe–target species. We previously have demonstrated that nonmagnetic species, such as bacterial and cells, can gain apparent magnetism by simply mixing with magnetic metal ions, such as Fe 3+ and Gd 3+ . The binding mechanism is based on the hard–soft acid–base (HSAB) theory .…”

Section: Introductionmentioning

confidence: 99%

Magnetic Graphene Oxide-Based Affinity Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Screening of Aflatoxin B1 from Complex Samples

2021

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Aflatoxin B1 (AFB1), commonly found in agriculture products, has been considered as a carcinogen. Thus, to develop analytical methods that can be used to rapidly screen the presence of AFB1 in complex samples is important. Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic materials as assisting materials to facilitate desorption/ionization of analytes. The feasibility of using GO as the affinity probe against AFB1 and as the assisting material in SALDI-MS analysis was first demonstrated. We also explored a facile method to impose magnetism on GO to generate magnetic GO (MGO) nanoprobes by simply incubating GO in aqueous FeCl3 under microwave heating. The generated MGO nanoprobes possessed magnetism and were capable of enriching trace AFB1 from complex samples. AFB1 enrichment took only 6 min by incubating MGO with samples under microwave heating (power = 90 W). Followed by magnetic isolation, the isolated conjugates were ready for SALDI-MS analysis. The enrichment steps including trapping and isolation can be completed within ∼10 min. The lowest detectable concentration of our method toward AFB1 was ∼1 nM. Results also showed that AFB1 can be selectively detected from complex samples, including cell lysates of fungal spores, AFB1-spiked peanut, and wheat samples, by using the developed method. The selectivity of our method against AFB1 from the samples containing other toxins including aflatoxin G1 and ochratoxin A was also examined. According to these results, we believe that the developed method should have the potential to be used for rapid screening of AFB1 from real-world samples.

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Using gadolinium ions as affinity probes to selectively enrich and magnetically isolate bacteria from complex samples

Cited by 13 publications

References 39 publications

Recent trends in sorbents for bioaffinity chromatography

Recent trends in sorbents for bioaffinity chromatography

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Magnetic Graphene Oxide-Based Affinity Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Screening of Aflatoxin B1 from Complex Samples

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