Using galactitol dehydrogenase coupled with water-forming NADH oxidase for efficient enzymatic synthesis of L-tagatose

Su, Wen-Bin; Li, Feilong; Li, Xueyong; Fan, Xue‐Gong; Liu, Ruijiang; Zhang, Ye-Wang

doi:10.1016/j.nbt.2021.01.003

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…At low substrate concentration, this relationship can be expressed as a simple linear proportional relationship. 24,25 However, at high substrate concentration, a complex relationship is extant that can be expressed in a number of ways.…”

Section: ■ Common Applications With Logarithm Of Concentrationmentioning

confidence: 99%

“…For both of these two kinds of enzymes, the relationship between enzymatic reaction rate and substrate concentration is often expressed by an enzymatic kinetics equation. At low substrate concentration, this relationship can be expressed as a simple linear proportional relationship. , However, at high substrate concentration, a complex relationship is extant that can be expressed in a number of ways.…”

Section: Common Applications With Logarithm Of Concentrationmentioning

confidence: 99%

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Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

et al. 2021

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The article is a response to a recent opinion piece that log concentration values should not be applied in analytical chemistry. An essential aim in the development of analytical chemistry methods is to obtain more sensitive and accurate detection values. For the application of chemical analysis methods, the obtained experiment data need to fit with the mathematical functions in the first place. As influenced by different detection principles and analytical methods, data can be displayed in a coordinate system with two linear axes for linear function fitting, or the data can first be taken through a logarithmic transformation and then for function fitting. Using raw data or data after logarithmic transformation primarily depends on analytical principles, without special rules of data formats. For example, ultraviolet− visible spectrophotometric data are more suitable for direct linear fitting. However, enzyme-catalyzed reaction or electrochemical data in logarithmic form are more appropriate for function fitting. This transformation of data form will not affect the soundness of fit statistics; rather, it simplifies the complexity of function analysis and calculation, which are the essence of analytical chemistry. In this brief article, we provide justification and legitimacy of the application of logarithmic processing in various fields of quantitative analytical chemistry.

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Section: ■ Common Applications With Logarithm Of Concentrationmentioning

confidence: 99%

Section: Common Applications With Logarithm Of Concentrationmentioning

confidence: 99%

Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

et al. 2021

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“…Another such example is the recent study by Su et al, whose objective was to produce l-tagatose from d-galactitol using galactitol dehydrogenase. [93] An efficient cofactor regeneration scheme using NADH oxidase as the regenerating enzyme enabled the authors to achieve high productivity using 3 mm cofactor per 100 mm of substrate. Asymmetric transformations could benefit immensely from this kind of enzyme-catalyzed cascade reactions, using the right choice of regenerating enzyme with similar initial rate of substrate conversion using innocuous substrates that can also facilitate downstream separation.…”

Section: Multienzymatic Cascade Using Other Redox Enzymesmentioning

confidence: 99%

“…In this case the oxidized cofactor was regenerated by simultaneous reduction of the dye 2,6‐dichlorophenolindophenon catalyzed by lipoamide dehydrogenase. Another such example is the recent study by Su et al., whose objective was to produce l ‐tagatose from d ‐galactitol using galactitol dehydrogenase [93] . An efficient cofactor regeneration scheme using NADH oxidase as the regenerating enzyme enabled the authors to achieve high productivity using 3 m m cofactor per 100 m m of substrate.…”

Section: Enzymatic Regenerationmentioning

confidence: 99%

Redox Biocatalysis: Quantitative Comparisons of Nicotinamide Cofactor Regeneration Methods

2022

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Enzymatic processes, particularly those capable of performing redox reactions, have recently been of growing research interest. Substrate specificity, optimal activity at mild temperatures, high selectivity, and yield are among the desirable characteristics of these oxidoreductase catalyzed reactions. Nicotinamide adenine dinucleotide (phosphate) or NAD(P)H‐dependent oxidoreductases have been extensively studied for their potential applications like biosynthesis of chiral organic compounds, construction of biosensors, and pollutant degradation. One of the main challenges associated with making these processes commercially viable is the regeneration of the expensive cofactors required by the enzymes. Numerous efforts have pursued enzymatic regeneration of NAD(P)H by coupling a substrate reduction with a complementary enzyme catalyzed oxidation of a co‐substrate. While offering excellent selectivity and high total turnover numbers, such processes involve complicated downstream product separation of a primary product from the coproducts and impurities. Alternative methods comprising chemical, electrochemical, and photochemical regeneration have been developed with the goal of enhanced efficiency and operational simplicity compared to enzymatic regeneration. Despite the goal, however, the literature rarely offers a meaningful comparison of the total turnover numbers for various regeneration methodologies. This comprehensive Review systematically discusses various methods of NAD(P)H cofactor regeneration and quantitatively compares performance across the numerous methods. Further, fundamental barriers to enhanced cofactor regeneration in the various methods are identified, and future opportunities are highlighted for improving the efficiency and sustainability of commercially viable oxidoreductase processes for practical implementation.

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“…In our previous work, galactitol dehydrogenase (Gdh) and NADH oxidase (Nox) were coupled for the preparation of l-tagatose [30]. The catalytic conditions were further optimized to attain highly efficient NAD + regeneration and l-tagatose production.…”

Section: Introductionmentioning

confidence: 99%

Preparation of combined cross-linked enzyme aggregates containing galactitol dehydrogenase and NADH oxidase for l-tagatose synthesis via in situ cofactor regeneration

Using galactitol dehydrogenase coupled with water-forming NADH oxidase for efficient enzymatic synthesis of L-tagatose

Cited by 10 publications

References 37 publications

Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

Logarithmic Data Processing Can Be Used Justifiably in the Plotting of a Calibration Curve

Redox Biocatalysis: Quantitative Comparisons of Nicotinamide Cofactor Regeneration Methods

Preparation of combined cross-linked enzyme aggregates containing galactitol dehydrogenase and NADH oxidase for l-tagatose synthesis via in situ cofactor regeneration

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