“…We combined Ab initio and homology-based prediction methods to construct consensus gene models. We used PASA (PASA, RRID: SCR_014656) (Haas et al, 2003), Genscan (GENSCAN, RRID: SCR_012902) (Burge and Karlin, 1997), Augustus (Augustus: Gene Prediction, RRID: SCR 008417) (Stanke et al, 2006), GlimmerHMM (GlimmerHMM, RRID: SCR_002654) (Majoros et al, 2004), GeneID (GeneID, SCR_002473) (Alioto et al, 2018), and SNAP (Korf, 2004) to search for gene models. Using the homology-based method, protein sequences of Caenorhabditis elegans (nematode, GCA_000002985.3), Capitella teleta (Marine worm, GCA_000328365.1), and Helobdella robusta (freshwater leech, GCA_000326865.1) were downloaded from the NCBI GenBank database (GenBank, RRID: SCR_002760) (Benson et al, 2014) and implemented for library construction.…”