This paper aims to provide a sound estimation of the true value and proportion of white matter (WM), gray matter (GM), and cerebrospinal fluid (CSF) of the brain DTI images for a proper 3D volume reconstruction. During the pre-processing stage, two nonlinear filters are operated, i.e. bilateral and anisotropic diffusion. The segmentation of each brain tissue is performed using the k-means clustering algorithm. To minimize filters bias and for obtaining the best reproducible results, a statistical analysis has been performed. Thus, the skewness and kurtosis statistics features were computed for each segmented brain tissue and filter. The fuzzy k-means method allows for clustering analysis and the Bland-Altman analysis investigates the agreement between two filtering techniques of the same statistics feature and brain tissue. Then the 3D reconstruction method is presented using ImageJ and the image stacks for raw and processed data. We conclude that anisotropic diffusion filter offers the best results and 3D reconstruction of brain tissues is feasible.