Using high-performance liquid chromatography/mass spectrometry for the quantification of plasma phospholipids in children with type 1 diabetes

Akmurzina, V. A.; Petryaikina, E. E.; Saveliev, S. V.; Селищева, А. А.

doi:10.1134/s1061934813140025

Cited by 3 publications

(4 citation statements)

References 21 publications

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“…Phosphatidylcholines (PCs) are the most abundant phospholipid species in human plasma ( 1 , 2 ). Changes in the PC species of various tissues are linked to cardiovascular disease, diabetes, and cancer ( 3 – 7 ). A classical method for the analysis of PCs is HPLC in connection with MS detection with ESI ( 2 , 7 , 8 ).…”

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confidence: 99%

“…Changes in the PC species of various tissues are linked to cardiovascular disease, diabetes, and cancer ( 3 – 7 ). A classical method for the analysis of PCs is HPLC in connection with MS detection with ESI ( 2 , 7 , 8 ). Progress in MS instrumentation has enabled the replacement of the separation step by direct injection of a sample into the mass spectrometer, also known as shotgun lipidomics ( 9 , 10 ).…”

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confidence: 99%

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Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer

Žáček

Bukowski

Rosenberger

et al. 2016

Journal of Lipid Research

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Phosphatidylcholine (PC) species in human plasma are used as biomarkers of disease. PC biomarkers are often limited by the inability to separate isobaric PCs. In this work, we developed a targeted shotgun approach for analysis of isobaric and isomeric PCs. This approach is comprised of two MS methods: a precursor ion scanning (PIS) of mass m/z 184 in positive mode (PIS m/z +184) and MS3 fragmentation in negative mode, both performed on the same instrument, a hybrid triple quadrupole ion-trap mass spectrometer. The MS3 experiment identified the FA composition and the relative abundance of isobaric and sn-1, sn-2 positional isomeric PC species, which were subsequently combined with absolute quantitative data obtained by PIS m/z +184 scan. This approach was applied to the analysis of a National Institute of Standards and Technology human blood plasma standard reference material (SRM 1950). We quantified more than 70 PCs and confirmed that a majority are present in isobaric and isomeric mixtures. The FA content determined by this method was comparable to that obtained using GC with flame ionization detection, supporting the quantitative nature of this MS method. This methodology will provide more in-depth biomarker information for clinical and mechanistic studies.

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confidence: 99%

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confidence: 99%

Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer

Žáček

Bukowski

Rosenberger

et al. 2016

Journal of Lipid Research

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“…There are several analytical methods available to analyze phosphatidylcholines in different biological matrices including bronchoalveolar lavage fluid, − exhaled breath condensate (EBC), exhaled particles, and oral fluid (saliva) . Analytical techniques for analysis of PCs include electrospray ionization mass spectrometry (ESI-MS), ,,− liquid chromatography/mass spectrometry (LC/MS), − ultrahigh performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC/qTOF-MS), , matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), time-of-flight secondary ion mass spectrometry (TOF-SIMS) . Most of these methods are based on direct infusion into the mass spectrometer or nonspecific separation of PCs without full validation of quantification.…”

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confidence: 99%

Measurement of Lung Phosphatidylcholines in Exhaled Breath Particles by a Convenient Collection Procedure

2015

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An analytical method based on high-performance liquid chromatography coupled to quadrupole tandem mass spectrometry was developed for the quantitative determination of four phosphatidylcholines (PCs) in human exhaled breath particles. Analytes were conveniently collected on an electrostatic polymer filter and extracted with methanol prior to analysis. Chromatographic separation was performed on an ultraperformance liquid chromatographic ethylene bridged hybrid phenyl column using a mobile phase consisting of water and methanol containing 4 mM ammonium formate and 0.1% ammonia. The mass spectrometer operated in positive electrospray ionization and selected reaction monitoring mode. Detection limits for PC 16:0/16:0 (dipalmitoylphosphatidylcholine, DPPC), PC 16:0/18:1, PC 16:0/18:2, and PC 18:0/18:2 were <0.01 ng/filter. Method recoveries at concentration levels of 0.1 and 10 ng/filter were 100-110% and 101-121%, respectively. Acceptable precision with coefficients of variation <20% and accuracies of 100% ± 20% were achieved. Identification of the individual PCs was performed on the basis of two product ions with correct ion ratios and chromatographic retention times. The highest amount in exhaled breath was found for DPPC with median concentration 1.14 ng/filter (range 0.6-21 ng/filter), and median molar ratios of DPPC/PC (16:0/18:1) of 1.98 (range 0.48-2.75). A different pattern with lower molar ratio (∼0.15) was found for oral fluid. The most significant element of this study was to use a precolumn in the LC system and to collecting exhaled particles in an electret polymer filter. Due to chromatographic interference by background contamination, an isolator column (PFC kit) was installed in between eluent mixer and injector to reduce contamination. This is the first LC/MS study where the method was successfully applied to analyze PCs in human exhaled breath by using a simple and convenient collection procedure.

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“…Finally, 18 publications related to metabolic perturbations in childhood T1D were included in the following quantitative assessment (Table 1). These 18 studies were designed to describe changes of metabolites or lipids in the blood samples from either pediatric IA, PT1D groups, or T1D children under different conditions, including positive detection of autoantibodies, diagnosis of T1D, and good or poor glycemic control [8,[12][13][14][15][21][22][23][24][25][26][27][28][29][30][31][32][33]. Among them, six were targeted at metabolic disorders under different glycemic control conditions to reflect the management effect, possible complications, and prognosis of T1D [21-23, 26, 31, 33].…”

Section: An Overview Of Metabolomics and Lipidomics Studies Onmentioning

confidence: 99%

Metabolomics and Lipidomics Studies in Pediatric Type 1 Diabetes: Biomarker Discovery for the Early Diagnosis and Prognosis

et al. 2023

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Aim. Type 1 diabetes (T1D) is an autoimmune disease with heterogeneous risk factors. Metabolic perturbations in the pathogenesis of the disease are remarkable to illuminate the interaction between genetic and environmental factors and how islet immunity and overt diabetes develop. This review aimed to integrate the metabolic changes of T1D to identify potential biomarkers for predicting disease progression based on recent metabolomics and lipidomics studies with parallel methodologies. Methods. A total of 18 metabolomics and lipidomics studies of childhood T1D during the last 15 years were reviewed. The metabolic fingerprints consisting of 41 lipids and/or metabolite classes of subjects with islet autoantibodies, progressors of T1D, and T1D children were mapped in four-time dimensions based on a tentative effect-score rule. Results. From birth, high-risk T1D subjects had decreased unsaturated triacylglycerols, unsaturated phosphatidylcholines (PCs), sphingomyelins (SMs), amino acids, and metabolites in the tricarboxylic acid (TCA) cycle. On the contrary, lysophosphatidylcholines (LPCs) and monosaccharides increased. And LPCs and branched-chain amino acids (BCAAs) were elevated before the appearance of islet autoantibodies but were lowered after seroconversion. Choline-related lipids (including PCs, SMs, and LPCs), BCAAs, and metabolites involved in the TCA cycle were identified as consensus biomarkers potentially predicting the development of islet autoimmunity and T1D. Decreased LPCs and amino acids indicated poor glycemic control of T1D, while elevated lysophosphatidylethanolamines and saturated PCs implied good glycemic control. Further pathway analysis revealed that biosynthesis of aminoacyl-tRNA, BCAAs, and alanine, aspartate, and glutamate metabolism were significantly enriched. Moreover, established cohort studies and predictive statistical models of pediatric T1D were also summarized. Conclusion. The metabolic profile of high-risk T1D subjects and patients demonstrated significant changes compared with healthy controls. This integrated analysis provides a comprehensive overview of metabolic features and potential biomarkers in the pathogenesis and progression of T1D.

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Using high-performance liquid chromatography/mass spectrometry for the quantification of plasma phospholipids in children with type 1 diabetes

Cited by 3 publications

References 21 publications

Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer

Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer

Measurement of Lung Phosphatidylcholines in Exhaled Breath Particles by a Convenient Collection Procedure

Metabolomics and Lipidomics Studies in Pediatric Type 1 Diabetes: Biomarker Discovery for the Early Diagnosis and Prognosis

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