Deep geothermal energy represents a key element of future renewable energy production due to its base load capability and the almost inexhaustible resource base. Especially with regard to heat supply, this technology offers a huge potential for carbon saving. One of the main targets of geothermal projects in Central Europe is the Upper Rhine Graben, which exhibits elevated subsurface temperatures and reservoirs with favorable hydraulic properties. Several decades of intensive research in the region resulted in a comprehensive understanding of the geological situation. This review study summarizes the findings relevant to deep geothermal projects and thus provides a useful working and decision-making basis for stakeholders. A total of nine geological units have been identified that are suitable for deep geothermal exploitation, comprising the crystalline basement, various sandstone formations and Mesozoic carbonates. An extensive lithostratigraphic, structural, geochemical, hydraulic and petrophysical characterization is given for each of these potential reservoirs. This paper furthermore provides an overview of the available data and geological as well as temperature models.