Using hormone-treated pregnant cows as a potential source of oocytes for in vitro fertilization

Ryan, David P.; Blakewood, E.G.; Swanson, W. F.; Rodriguez, H.F.; Godke, R.A.

doi:10.1016/0093-691x(93)90372-c

Cited by 17 publications

(5 citation statements)

References 32 publications

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“…This was substantiated by the similar conception rates between non-aspirated and aspirated cows on day 30 and between days 60 and 90 post TAI. This aggregated new information on the previous studies (Ryan et al 1993;Meintjes et al 1995;Ooe et al 1997), which did not detect any adverse effect of ovum pickup during early gestation.…”

Section: Discussionmentioning

confidence: 58%

Impact of Probing the Reproductive Tract During Early Pregnancy on Fertility of Beef Cows

Pugliesi

Scolari

Mesquita

et al. 2014

Reprod Domestic Animals

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This short communication reports the impact of endometrial biopsies, uterine flushings and follicular fluid aspiration procedures at day 6 post artificial insemination (AI) on pregnancy rates. In Experiment 1, cows were timed AI (TAI) and assigned to the following treatment groups: control (n = 37), uterine flushing (n = 35) and endometrial biopsy (n = 38). On day 30 post AI, pregnancy rates were 40.5%, 33% and 28.5%, respectively (p > 0.1). Pregnancy rate on day 60 was lower (p < 0.004) in flushed cows than in the controls. In Experiment 2, oestrus was detected and cows were assigned to flushing (n = 32) or biopsy (n = 33) treatments 6 days after AI, which resulted in pregnancy rates of 31% and 36%, respectively (p > 0.1). In Experiment 3, cows were, 6 days after TAI, randomly assigned to the following treatments: control (n = 84) or aspiration of the largest follicle (n = 73). Pregnancy rates on day 30 post AI were 63.5% for the control group and 53% for the aspirated group (p > 0.1). In conclusion, uterine flushing and endometrial biopsy negatively affect pregnancy rates, but neither procedure can be considered to be incompatible with pregnancy maintenance. Follicular aspiration during pregnancy does not interact with pregnancy success. The amount and quality of samples obtained are compatible with the use of cellular and molecular analysis of uterine variables from cows that failed or succeeded on maintaining pregnancy.

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Section: Discussionmentioning

confidence: 58%

Impact of Probing the Reproductive Tract During Early Pregnancy on Fertility of Beef Cows

Pugliesi

Scolari

Mesquita

et al. 2014

Reprod Domestic Animals

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“…It has been suggested that repeated OPU twice a week can lead to maximum production of culture-competent oocytes because performance of follicle aspiration frequently enough to induce a newly recruited cohort of follicles and to prevent development of a dominant follicle yields a higher number of harvestable follicles and oocytes [5,13,[19][20][21]. Oocyte collection by OPU from pregnant cows is often reported, and this procedure has not been shown to cause pregnancy loss [9][10][11]22]. It is well known that repeated OPU at short intervals is possible [10,19,23].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, OPU is applicable to animals in various reproductive states, including the cyclic, non-cyclic and early pregnancy states [9][10][11][12][13].…”

mentioning

confidence: 99%

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Hirata

Sato

Sasaki

et al. 2008

Journal of Reproduction and Development

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Abstract. We investigated whether suckling would affect embryo production of cows bred by timed artificial insemination (TAI) following an ovulation synchronization protocol combined with ovum pick-up and progesterone releasing intravaginal device (OPU-PRID-TAI protocol). The number of oocytes and transferable embryos collected by repeated OPU, performed before and after TAI, were recorded. A total of 14 Japanese Black cows were divided into weaned (n=7) and suckled groups (n=7). All 14 cows were treated with OPU on day 0 (the first day of treatment) and then with a PRID for 9 days. Prostaglandin F2α analog was administered on day 7, GnRH analog was administered on day 10 (36 h after removal of the PRID) and TAI was performed 12 h later. Ovulation was confirmed by palpation per rectum the following day. After TAI, additional OPU sessions were performed on days 18, 25 and 32. The synchronized ovulation rates of the weaned and suckled groups were 100 and 85.7%, and the conception rates were 71.4 and 42.9%, respectively. Immature oocytes were fertilized and cultured in vitro. The numbers of oocytes collected and blastocysts generated were similar between the individual OPU sessions in both groups. However, the total numbers of oocytes collected, cultured oocytes, cleavage embryos and blastocysts as well as the proportions of cleavage embryos and blastocysts to cultured oocytes were all significantly (P<0.05) greater in the weaned group compared with the suckled group. These results suggest that the OPU-PRID-TAI protocol has the potential to produce a significant number of good-quality embryos in vitro after repeated OPU in early postpartum weaned Japanese Black cows. To collect more oocytes and produce more embryos, we suggest that calves be removed from cows scheduled for treatment using this protocol. Key words: Cow, Early postpartum, Japanese black, Ovum pick-up (OPU), Suckling, Timed artificial insemination (TAI) (J. Reprod. Dev. 54: [346][347][348][349][350][351] 2008) t is economically advantageous for beef cattle producers to have cows in estrus within 60 days postpartum and pregnant within 80 days postpartum and to produce a calf each year. An increase in days open results in a significant economic loss, particularly in the case of Japanese Black cows whose calves have a high market value because of the marbled meat they produce. Various estrus and ovulation synchronization programs have been applied to early postpartum beef cows to achieve maximum reproductive ability [1][2][3]. Use of intravaginal progesterone devices in these programs has been effective in animals with silent heat or anestrus [4].Transvaginal ovum pick-up (OPU), a technique for collecting oocytes from live donor cows using ultrasound-guided transvaginal oocyte aspiration, and in vitro embryo production (IVP) have become important methods for making the most of the genetic traits of superior donors in the beef industry [5][6][7][8]. In addition, OPU is applicable to animals in various reproductive states, including the cyclic, non-cycl...

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“…However, OTU can also be applied in pregnant cows, in which collection of oocytes by OPU is possible in the first three months of pregnancy (72)(73)(74)(75)(76). Treatment and sampling of ovaries can be carried out with surgical techniques (77), but these invasive procedures are impractical for serial treatment and sampling on a regular basis.…”

Section: Relevance Of Using Otu As Research Tool In Bovine Reproductionmentioning

confidence: 99%

Biomedical Applications of Ovarian Transvaginal Ultrasonography in Cattle

Velazquez

Kues

Niemann

2014

Animal Biotechnology

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Ovarian transvaginal ultrasonography (OTU) has been used world-wide for commercial ovum pick-up programs for in vitro embryo production in elite herds, providing an excellent model for the elucidation of factors controlling bovine oocyte developmental competence. Noninvasive sampling and treatment of ovarian structures is easily accomplished with bovine OTU techniques providing a promising system for in vivo delivery of transgenes directly into the ovary. The current review summarizes existing bovine OTU models and provides prospective applications of bovine OTU to undertake research in reproductive topics of biomedical relevance, with special emphasis on the development of in vivo gene transfer strategies.

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Using hormone-treated pregnant cows as a potential source of oocytes for in vitro fertilization

Cited by 17 publications

References 32 publications

Impact of Probing the Reproductive Tract During Early Pregnancy on Fertility of Beef Cows

Impact of Probing the Reproductive Tract During Early Pregnancy on Fertility of Beef Cows

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Biomedical Applications of Ovarian Transvaginal Ultrasonography in Cattle

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