Despite over a century of research, the physics of galaxy rotation is not yet fully understood, and there is a clear discrepancy between the observed mass of galaxies and their rotational velocity. Here, we report on another observation of tension between the physical properties of galaxies and their rotational velocity. We compare the apparent magnitude of galaxies and find a statistically significant asymmetry between galaxies that rotate in the same direction relative to the Milky Way and galaxies that rotate in the opposite direction relative to the Milky Way. While asymmetry in the brightness is expected due to the Doppler shift effect, such asymmetry is expected to be subtle. The observations shown here suggest that the magnitude difference is sufficiently large to be detected by Earth-based telescopes. The asymmetry is consistent in both the northern and southern galactic poles. The difference is also consistent across several different instruments such as DECam, SDSS, Pan-STARRS, and HST as well as different annotation methods, which include automatic, manual, or crowdsourcing annotations through “Galaxy Zoo”. The observation can also explain other anomalies such as the Ho tension. Analysis of Ia supernovae where the host galaxies rotate in the same direction relative to the Milky Way shows a much smaller tension with the Ho value as estimated by the CMB.