Using HPLC and multivariate analyses to investigate variations in the polyphenolic compounds as well as antioxidant and antiglycative activities of some Lamiaceae species native to Iran

Rahimmalek, Mehdi; Afshari, Mahvash; Sarfaraz, Danial; Miroliaei, Mehran

doi:10.1016/j.indcrop.2020.112640

Cited by 28 publications

(26 citation statements)

References 44 publications

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“…Furthermore, a higher amount of TPC was observed in oregano from the Atacama than for the same species cultivated in other world regions [ 24 , 25 ]. While the total flavonoid content (593 ± 30.50 mg QE/100 g DW), were also close to the values reported for the aerial parts of the oregano from northern Iran (521 to 466 mg QE/100 g DW) [ 26 ]. FRAP analysis revealed oregano extract as showing the strongest reducing power than Rheum rhabarbarum [ 23 ].…”

Section: Resultssupporting

confidence: 84%

UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities

et al. 2021

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The Lamiaceae family is an important source of species among medicinal plants highly valued for their biological properties and numerous uses in folk medicine. Origanum is one of the main genera that belong to this family. The purpose of the study was to determine the phenolic composition of the Origanum vulgare extract and evaluate the antimicrobial, antioxidant, and inhibitory activities of this species that grows in the Andean region of the Atacama Desert. High-performance liquid chromatography was performed to determine the main phenols. Rosmarinic acid was identified as the predominant phenolic compound in this species (76.01 mg/100 g DW), followed by protocatechuic acid, which to our knowledge, no previous study reported similar concentrations in O. vulgare. The oregano extract exhibited a content of total phenolic (3948 mg GAE/100 g DW) and total flavonoid (593 mg QE/100 g DW) with a higher DPPH antioxidant activity (IC50 = 40.58 µg/mL), compared to the same species grown under other conditions. Furthermore, it was found to inhibit α-glucosidase activity with an IC50 value (7.11 mg/mL) lower than acarbose (129.32 mg/mL). Pseudomonas syringae and Pantoea agglomerans (both MIC 0.313 mg/mL and MBC 1.25 mg/mL) were the bacteria most susceptible to oregano extract with the lowest concentration necessary to inhibit bacterial growth. These results open the door for the potential use of this plant to manage chronic diseases, and they expand the knowledge of the species cultivated in arid environmental conditions.

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Section: Resultssupporting

confidence: 84%

UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities

et al. 2021

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“…On the contrary, there are many studies in which the phenolic contents of the extracts obtained with solvents such as water, ethanol, methanol, and ethyl acetate of other Satureja species are illuminated. Rosmarinic acid was reported to be the predominant phenolic compound in most of these studies presented (Alonso‐Carrillo et al., 2017; Bektasevic et al., 2017; Fatemi et al., 2019; Mašković et al., 2017; Rahimmalek et al., 2020). In our study, rosmarinic acid was detected (59.95 µg phenolic/g extract), but it was found to be lower than the dominant phenolics referred above.…”

Section: Resultsmentioning

confidence: 96%

HIV‐1‐RT inhibition activity ofSatureja spicigera(C.KOCH) BOISS. Aqueous extract and docking studies of phenolic compounds identified by RP‐HPLC‐DAD

Bektaş

Şahin

Beldüz

et al. 2021

Journal of Food Biochemistry

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AIDS is a global disease caused by HIV, affecting millions of people and causing death.The current limitations of antiretroviral therapy used in the therapy of HIV/AIDS have led to the need to search for new and effective drugs from natural products, especially plants. Herewith, using the present study, the detection of HIV-1-RT inhibition of aqueous extract of Satureja spicigera (C.KOCH) BOISS. was performed for the first time. Besides, total phenolic content (TPC), analysis of phenolic constituents by RP-HPLC-DAD and antioxidant capacity by DPPH and Ferric reducing antioxidant power (FRAP) methods were determined for the first time. In addition, molecular docking studies were carried out between HIV-1-RT and phenolic substances, the presence of which was determined in the aqueous extract, for the determination of the phenolics that may be responsible for HIV-1-RT activity. HIV-1-RT inhibition was defined as IC 50 : 22.83 μg/ml. Benzoic acid, vanillin, rutin, and chlorogenic acid were present as main phenolics in quantities of 621.96, 505.87, 349.33, and 323.23 µg phenolic/g extract, respectively. Further, TPC, DPPH, and FRAP were calculated as in the order of 151.69 mg GAE/g extract, 23.77 µg/ml, and 445.7 µmol TE/g extract. Chlorogenic acid (−8.48 kcal/mol) was found to be the most effective ligand in docking studies, with a value close to positive standard nevirapine (−9.35 kcal/mol). Hereby, although the aqueous extract of S. spicigera can be used as a natural antioxidant, the crude extract or its phenolics have the potential to be used in the treatment of AIDS due to its high HIV-1-RT activity. Practical applicationsIn this study, anti-HIV-1-RT and antioxidant activity and total phenolic content of Satureja spicigera aqueous extract were determined. In addition, HPLC analysis of some phytochemicals and the activities of these phytochemicals against HIV-1-RT enzyme was determined by molecular docking studies. The results showed that the aqueous extract of S. spicigera and some of the phytochemicals it contains have the potential to be used as a natural product against HIV infection or in the treatment of AIDS.

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“…They reported that the main components were luteolin 4-glucoside, luteolin 7-glucoside and apigenin 7-glucoside and these compounds exhibited significant DPPH radical scavenging activity. The antioxidant activity of rosmarinic acid has been reported by many researchers [ 35 , 36 , 37 , 38 , 39 ]. Therefore, rosmarinic acid was considered to have a positive effect on antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

İstifli

2021

Molecules

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The aim of this study was to investigate the chemical composition, antioxidant and enzyme inhibitory activities of methanol (MeOH) extracts from Onosma bourgaei (Boiss.) and O. trachytricha (Boiss.). In addition, the interactions between phytochemicals found in extracts in high amounts and the target enzymes in question were revealed at the molecular scale by performing in silico molecular docking simulations. While the total amount of flavonoid compounds was higher in O. bourgaei, O. trachytricha was richer in phenolics. Chromatographic analysis showed that the major compounds of the extracts were luteolin 7-glucoside, apigenin 7-glucoside and rosmarinic acid. With the exception of the ferrous ion chelating assay, O. trachytricha exhibited higher antioxidant activity than O. bourgaei. O. bourgaei exhibited also slightly higher activity on digestive enzymes. The inhibitory activities of the Onosma species on tyrosinase were almost equal. In addition, the inhibitory activities of the extracts on acetylcholinesterase (AChE) were stronger than the activity on butyrylcholinesterase (BChE). Molecular docking simulations revealed that luteolin 7-glucoside and apigenin 7-glucoside have particularly strong binding affinities against ChEs, tyrosinase, α-amylase and α-glucosidase when compared with co-crystallized inhibitors. Therefore, it was concluded that the compounds in question could act as effective inhibitors on cholinesterases, tyrosinase and digestive enzymes.

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Using HPLC and multivariate analyses to investigate variations in the polyphenolic compounds as well as antioxidant and antiglycative activities of some Lamiaceae species native to Iran

Cited by 28 publications

References 44 publications

UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities

UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities

HIV‐1‐RT inhibition activity ofSatureja spicigera(C.KOCH) BOISS. Aqueous extract and docking studies of phenolic compounds identified by RP‐HPLC‐DAD

Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

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