Using in-ovo injection of olive pulp extract and vitamin C to improve hatchability, post hatch growth performance, carcass traits and some biochemical blood analysis in broiler chickens

Abstract

A total of 630 fresh hatching eggs containing live embryo at 14 day of incubation were distributed randomly into seven treatment groups (three replicates/treatment, 30eggs/each) and the fertile eggs were injected in the yolk sac as following groups: eggs were un –injected (T1), eggs were injected with hole only as negative control (T2), eggs were injected with distilled water as positive control (T3), eggs were injected with OPE at 500 and 1000ppm/egg, T4 and T5), while eggs were injected with Vit C at 4 and 8mg/egg (T6 and T7) respectively. The newly hatched chicks resulting from previous treatments were raised till 5 weeks old. Results showed that hatchability %, hatched chicks weight significantly improved for OPE (1000ppm/egg, T5) compared with vitamin C groups. However, performance of chicks resulting from injected eggs with (8 mg/egg, Vit C, T7) showed significantly (P≤0.001) increase in body weight (bwt), body weight gain (bwt) and feed intake than control groups. Measurements of carcass traits of OPE (1000ppm/egg) showed significant improvement in dressing weight when compared to those all injected control groups. Birds resulting from injected eggs with vit C treatments having higher plasma total protein and globulin level compared to others in non-injected control group. Additionally, in-ovo OPE (1000ppm/egg) injection resulted in boosted plasma antioxidants enzymes activities and significantly increased plasmaT4 concentrations compared with different control groups. In conclusion, in-ovo injection at day14 of incubation with either of OPE or vitamin C has positive effect on hatchability %, chick performance, dressing weight and anti-oxidative status of hatched chicks. Hence, in-ovo injection with OPE (1000ppm/egg) at14th day of incubation could be recommended for improving chick’s health.

Effect of in ovo injection of epigallocatechin-3 gallate and oleuropein on hatching, productive and physiological aspects of broiler chicks exposed to short heat stress

“…An absent information on the direct influence of IO of EG and OL extracts was documented, although their dietary effect was illustrated in literature. Inconsistent result was obtained recently by Khalifa et al (2023), that IO into YS at 14 days of incubation with 1000 ppm olive pulp extract did not change hatchability and BW whereas the dose of 500 ppm decreased the hatchability.…”

“…Both T3 and T4 are produced under control of TSH and are important in regulating metabolism, muscular growth, heat production, eggshell pipping during hatching and mobilization of glycogen and fat stores (Givisiez et al, 2020). Comparable results indicated that levels of T4 along with T3 in blood were enhanced in hatched chicks by IO of 1000 mg olive pulp extract (Khalifa et al, 2023) or 30 mg naringin (Ranjbar et al, 2019). Opposite data to us, it was confirmed that high amount of serum T3 in hatched chicks was registered by IO into AC site at 17.5 days of heat-stressed embryos with 6 mg of black cumin (Oke et al, 2021) or with 2 mg of clove (Akosile et al, 2023b).…”

“…Moreover, in heat-stressed quails, Orhan et al (2013) suggested that EG delivered in diet (200 or 400 mg/kg) suppressed at greater extent the stress-related gene expression and inflammatory markers of gene transcription in liver which involving 60, 70 and 90 HSP, cyclooxygenase-2 and activator protein-1 components (c-Jun and c-Fos). Identical result referred that antioxidative status of hatched chicks was enhanced at 5 weeks through feeding embryos by 1000 mg olive pulp which resulted in high catalase, SOD and TAC in muscles (Khalifa et al, 2023). The improvement in oxidant/antioxidant balance might be related to positive influence of OL to reduce body temperature of broilers reared in hot climate (Oke et al, 2017).…”

“…Nowadays, there are interesting works have been directed toward early delivering for phytochemicals extracted from potential plants to the growing embryos at different ages of incubation through application of IO. These bioactive compounds injected in ovo could mitigate the overall deleterious impacts during embryonic development through improving indigenous gut microflora (Hajati et al, 2014), immune state and redox system (Khalifa et al, 2023) which affect hatchability (Akosile et al, 2023a) and even modulating productive performance (Oke et al, 2021) and meat quality (Wei et al, 2011) in post hatch time of chicks.…”