Using in vitro embryo culture for obtaining new breeding forms of peach

Митрофанова, И.В.; Смыков, А. В.; Митрофанова, О.В.; Лесникова-Седошенко, Н.П.; Chirkov, Sergei; Zhdanova, Irina V.

doi:10.17660/actahortic.2020.1289.23

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Some morphometric parameters of embryo germination of the studied peach cross combinations, depending on the stratification duration and genotype, are presented in Table 1. Our previous studies on the isolated peach embryos culture revealed that Monnier medium was the most favorable one for the development of peach embryos [39]. The germination of hybrid embryos and further regeneration of plantlets in the studied peach cross combinations were induced on Monnier medium (Figure 1, Table 2).…”

Section: Embryoculturementioning

confidence: 80%

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

et al. 2021

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Peach [Prunus persica (L.) Batsch] is among the most demanded fruit crops in the world. Biotechnological methods help to originate new hybrid forms in order to increase the cultivar diversity and create new valuable genotypes. Cross combinations between the cultivars Clyde Wilson, Jerseyglo, Loadel, Summerglo and the promising cultivar ‘Nikitskiy Podarok’ have been done. The embryos of these hybrids germinated and formed plantlets after stratification at 4 °C for 45–60 days. The best regeneration rates in the hybrids ‘Loadel’ × ‘Nikitskiy Podarok’ and ‘Summerglo’ × ‘Nikitskiy Podarok’ (96.30% and 92.59%, respectively) were noted on hormone-free Monnier culture medium supplemented with 400.0 mg L−1 casein hydrolyzate. When the newly formed plantlets had necrosis of the shoot apex or immature roots, nodal shoot segments were used. At the same time, a high regeneration capacity was noted in the hybrids ‘Summerglo’ × ‘Nikitskiy Podarok’ and ‘Loadel’ × ‘Nikitskiy Podarok’ on B5 culture medium with 0.75 mg L−1 6–benzyl–aminopurine (BAP) + 0.1 mg L−1 indole–3–butyric acid (IBA). After the second subculture, the number of new adventitious shoots was 5.18 ± 0.18 and 4.95 ± 0.18 shoots per explant, respectively. The plants obtained from the hybrid embryos in a soil mixture soil: peat: sand (3:1:1) were adapted. The main morphological and anatomical features of the leaf blades in newly originated peach hybrids have been studied: the thickness of their tissues and the distribution of stomatal apparatus, as well as the physiological parameters of the photosystem II activity in regenerants cultured in vitro and during their in vivo acclimatization. The high capacity to post aseptic adaptation in the obtained hybrids has been shown.

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Section: Embryoculturementioning

confidence: 80%

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

et al. 2021

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“…In Russia, these two ilarviruses were detected on cultivated Prunus species by ELISA in the 2000s [12,13]. However, no genome of the Russian PDV or PNRSV isolates has been sequenced or characterized.…”

Section: Introductionmentioning

confidence: 99%

Whole Genome Characterization of Prunus Necrotic Ringspot Virus and Prune Dwarf Virus Infecting Stone Fruits in Russia

Chirkov,

Sheveleva,

Tsygankova

et al. 2023

Horticulturae

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We conducted a survey of the phytosanitary status of the Prunus germplasm collection in the Nikita Botanical Gardens, Yalta, Russia. The virome of plants displaying virus-like symptoms was studied using Illumina MiSeq high-throughput sequencing. Reads related to prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), and ourmia-like virus 1 (OuLV1) were generated in a number of samples. Near complete genomes of two divergent PNRSV isolates, PDV isolate, and a contig partly covered OuLV1 genome were assembled de novo using the metaSPAdes program. The structure of the genomic RNA1, RNA2, and RNA3 of the new ilarvirus isolates was shown to be typical of PNRSV and PDV. This is the first report and characterization of the PNRSV and PDV full-length genomes from Russia, expanding the information on their geographical distribution and genetic diversity. An open reading frames (ORF)-based phylogeny of all full-length PNRSV and PDV genomes available in GenBank divided each ORF into two or three main clusters. A number of isolates migrated from one cluster to another cluster, depending on the analyzed genome segment. The different branching order may indicate reassortment in the evolutionary history of some PDV and PNRSV isolates.

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“…В последние годы в селекции основных сельскохозяйственных растений все шире используют новые методы клеточной инженерии, в частности индукцию сомаклональных вариантов из каллусных культур [13][14][15], клеточную селекцию [16][17][18], культуру изолированных зародышей [19,20] и другие приемы [21]. Эти методы позволяют не только повысить эффективность селекционного процесса, но и получить принципиально новые ценные генотипы, которые будут использованы для создания высокопродуктивных, устойчивых к стрессовым факторам среды сортов [16,18,22].…”

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Creation of clary sage cultivar using cell engineering methods. 2. Study of plant-regenerants at the stages of breeding process

Stavtzeva¹,

Yegorova²

2021

TVAN

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Clary sage (Salvia sclarea L.) is a widely demanded essential oil plant. The products of its processing are used in medicine, perfumery and cosmetics, food industry, paint and varnish production. The main breeding tasks are to develop cultivars that combine high yield and quality of essential oil, resistant to abiotic and biotic stresses. All clary sage cultivars registered in the ‘State register of breeding achievements allowed for use’ Russian Federation were obtained on the basis of traditional methods. The initial breeding material of S. sclarea was obtained at the FSBSI “Research Institute of Agriculture of Crimea” using methods of cell engineering (induction of somaclones from callus cultures, obtaining hybrids in embryoculture and cell selection for resistance to osmotic stress). The aim of this work was to study the main economic and morphometric parameters of clary sage samples created using various biotechnological methods at the main stages of the breeding process. As a material for research, we used samples obtained in vitro using three biotechnological methods – regenerants (R2-R3) from calli of ‘C-785’ and ‘Taigan’ cultivars, obtained in isolated embryo culture hybrids (Salvia sclarea L. x S. grandiflora Etling.) and forms selected in embryoculture on a selective medium with an osmotic (mannitol or NaCl). When studying 10 sage samples (seed progeny of regenerants) in the breeding nursery (2012–2014), three that exceeded the control cultivar ‘Taigan’ in terms of basic economic characteristics were identified. The collection of essential oil from them was 1.2-1.4 times higher than in the control. In the nursery of competitive variety trials (2016–2019), when studying three samples (No. R3-1-6, 226-08 and 1-01), No. R3-1-6 was selected for a number of indicators. This sample is seed progeny of regenerant obtained by the induction of morphogenesis from callus. A characteristic feature of this sample was an increase in the number of lateral shoots per inflorescence by 41.1 %, which contributed to an increase in the mass of inflorescences. By the yield of inflorescences (162.0 c/ha) and the collection of essential oil (51.5 kg/ha), No. R3-1-6 exceeded control by 24.9 % and 43.9 %, respectively. The patent on the new clary sage cultivar ‘Selinzh’ (No. R3-1-6), in the creation of which the method of cell engineering was used for the first time, was received.

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Using in vitro embryo culture for obtaining new breeding forms of peach

Cited by 6 publications

References 25 publications

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

Use of Biotechnological Methods to Support the Production of New Peach Hybrids

Whole Genome Characterization of Prunus Necrotic Ringspot Virus and Prune Dwarf Virus Infecting Stone Fruits in Russia

Creation of clary sage cultivar using cell engineering methods. 2. Study of plant-regenerants at the stages of breeding process

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