Using internal transcribed spacers for Calanoida (Crustacea, Copepoda) species identification

Rocha, Gustavo Martins; Martinelli, Arturo B.; Utz, Laura R. P.; Fernandes, Luiz Fernando Loureiro

doi:10.1007/s12526-018-0902-9

Cited by 6 publications

(5 citation statements)

References 31 publications

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“…The relationship among phylogroups was unresolved probably due to the lower genetic variability and slower evolutionary rate of ITS2 , also demonstrated by low intraspecific genetic distances (Additional file 3 ). The findings agree with other studies that evaluated the diversity of this nuclear gene in crustaceans [ 47 , 60 – 62 ]. Obtained values of genetic distances within and between phylogroups were of intraspecific level compared to the interspecific distances found for other European Astacidae (e.g., 1–5% between sister species Pontastacus ( Astacus) pachypus and Pontastacus (Astacus) leptodactylus vs. 0.00–0.79% between phylogroup pairs in this study) [ 47 ].…”

Section: Discussionsupporting

confidence: 92%

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

et al. 2020

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Background Austropotamobius torrentium is a freshwater crayfish species native to central and south-eastern Europe, with an intricate evolutionary history and the highest genetic diversity recorded in the northern-central Dinarides (NCD). Its populations are facing declines, both in number and size across its entire range. By extanding current knowledge on the genetic diversity of this species, we aim to assist conservation programmes. Multigene phylogenetic analyses were performed using different divergence time estimates based on mitochondrial and, for the first time, nuclear DNA markers on the largest data set analysed so far. In order to reassess taxonomic relationships within this species we applied several species delimitation methods and studied the meristic characters with the intention of finding features that would clearly separate stone crayfish belonging to different phylogroups. Results Our results confirmed the existence of high genetic diversity within A. torrentium, maintained in divergent phylogroups which have their own evolutionary dynamics. A new phylogroup in the Kordun region belonging to NCD has also been discovered. Due to the incongruence between implemented species delimitation approaches and the lack of any morphological characters conserved within lineages, we are of the opinion that phylogroups recovered on mitochondrial and nuclear DNA are cryptic subspecies and distinct evolutionary significant units. Conclusions Geographically and genetically isolated phylogroups represent the evolutionary legacy of A. torrentium and are highly relevant for conservation due to their evolutionary distinctiveness and restricted distribution.

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Section: Discussionsupporting

confidence: 92%

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

et al. 2020

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“…ITS is a degraded region during maturation in ribosomal transcript. (Choudhary et al, 2015) ITS due to more variation properties than ribosomal sequences have been commonly used for molecular markers to figure out phylogenetic analysis and/or identification of bacteria, plant and animal strains and/or species (Lee et al, 2017;Rocha et al, 2019;Ali et al, 2019;Fazeli-Nasab et al, 2020). Pourahmad et al (2019) performed sequence analysis of the mycobacterial 16S-23S ITS region to determine aquatic mycobacteria species, reporting effectiveness of this marker.…”

Section: Its (Internal Transcribed Spacer)mentioning

confidence: 99%

Application of different molecular markers in biotechnology

Kocaman

Toy

Marakli

2020

International Journal of Science Letters

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“…An alternative approach to microscopy for direct monitoring of salmon louse larvae is the use of molecular methods. Recently, DNA‐based identification methods are rising in prevalence as molecular techniques can be a more rapid and accurate approach, particularly in distinguishing species that are morphologically similar (Goffredi et al, 2006; Henzler et al, 2010; Komai et al, 2019; McManus & Katz, 2009; Rocha et al, 2019). Molecular methods reduce the operator dependency and potential error that is associated with manual counts, and are theoretically very precise (i.e.…”

Section: Introductionmentioning

confidence: 99%

Finding the needle in the haystack: Comparison of methods for salmon louse enumeration in plankton samples

Bui

Dalvin

Vågseth

et al. 2021

Aquaculture Research

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Using internal transcribed spacers for Calanoida (Crustacea, Copepoda) species identification

Cited by 6 publications

References 31 publications

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

Application of different molecular markers in biotechnology

Finding the needle in the haystack: Comparison of methods for salmon louse enumeration in plankton samples

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