Using Live Imaging to Examine Early Cardiac Development in Zebrafish

McCann, Tess; Shrestha, Rabindra Man; Graham, Alexis; Bloomekatz, Joshua

doi:10.1007/978-1-0716-2035-9_9

Cited by 3 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For live imaging, Tg(myl7:eGFP) embryos were exposed to DMSO or 20µM LY at bud stage and mounted at 12 somite stage as described (84). Mounted embryos were covered with 0.1% DMSO/20µM LY in Tricaine-E3 solution and imaged using a Leica SP8 X microscope with a HC PL APO 20X/0.75 CS2 objective in a chamber heated to 28.5 ºC.…”

Section: Methodsmentioning

confidence: 99%

The myocardium utilizes Pdgfra-PI3K signaling to steer towards the midline during heart tube formation

Shrestha

McCann

Saravanan

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Coordinated cell movement is a fundamental process in organ formation. During heart development, bilateral myocardial precursors collectively move towards the midline (cardiac fusion) to form the primitive heart tube. Along with extrinsic influences such as the adjacent anterior endoderm which are known to be required for cardiac fusion, we previously showed that the platelet-derived growth factor receptor alpha (Pdgfra) is also required. However, an intrinsic mechanism that regulates myocardial movement remains to be elucidated. Here, we uncover an essential intrinsic role in the myocardium for the phosphoinositide 3-kinase (PI3K) intracellular signaling pathway in directing myocardial movement towards the midline. In vivo imaging reveals that in PI3K-inhibited zebrafish embryos myocardial movements are misdirected and slower, while midline-oriented dynamic myocardial membrane protrusions become unpolarized. Moreover, PI3K activity is dependent on and genetically interacts with Pdgfra to regulate myocardial movement. Together our findings reveal an intrinsic myocardial steering mechanism that responds to extrinsic cues during the initiation of cardiac development.

show abstract

Section: Methodsmentioning

confidence: 99%

The myocardium utilizes Pdgfra-PI3K signaling to steer towards the midline during heart tube formation

Shrestha

McCann

Saravanan

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Preclinical Models of Cardiac Disease: A Comprehensive Overview for Clinical Scientists

van Doorn,

Amesz,

Sadeghi

et al. 2024

Cardiovasc Eng Tech

View full text Add to dashboard Cite

For recent decades, cardiac diseases have been the leading cause of death and morbidity worldwide. Despite significant achievements in their management, profound understanding of disease progression is limited. The lack of biologically relevant and robust preclinical disease models that truly grasp the molecular underpinnings of cardiac disease and its pathophysiology attributes to this stagnation, as well as the insufficiency of platforms that effectively explore novel therapeutic avenues. The area of fundamental and translational cardiac research has therefore gained wide interest of scientists in the clinical field, while the landscape has rapidly evolved towards an elaborate array of research modalities, characterized by diverse and distinctive traits. As a consequence, current literature lacks an intelligible and complete overview aimed at clinical scientists that focuses on selecting the optimal platform for translational research questions. In this review, we present an elaborate overview of current in vitro, ex vivo, in vivo and in silico platforms that model cardiac health and disease, delineating their main benefits and drawbacks, innovative prospects, and foremost fields of application in the scope of clinical research incentives.

show abstract

The myocardium utilizes a platelet-derived growth factor receptor alpha (Pdgfra)–phosphoinositide 3-kinase (PI3K) signaling cascade to steer toward the midline during zebrafish heart tube formation

Shrestha,

McCann,

Saravanan

et al. 2023

eLife

View full text Add to dashboard Cite

Coordinated cell movement is a fundamental process in organ formation. During heart development, bilateral myocardial precursors collectively move toward the midline (cardiac fusion) to form the primitive heart tube. Extrinsic influences such as the adjacent anterior endoderm are known to be required for cardiac fusion. We previously showed however, that the platelet-derived growth factor receptor alpha (Pdgfra) is also required for cardiac fusion (Bloomekatz et al., 2017). Nevertheless, an intrinsic mechanism that regulates myocardial movement has not been elucidated. Here, we show that the phosphoinositide 3-kinase (PI3K) intracellular signaling pathway has an essential intrinsic role in the myocardium directing movement toward the midline. In vivo imaging further reveals midline-oriented dynamic myocardial membrane protrusions that become unpolarized in PI3K-inhibited zebrafish embryos where myocardial movements are misdirected and slower. Moreover, we find that PI3K activity is dependent on and interacts with Pdgfra to regulate myocardial movement. Together our findings reveal an intrinsic myocardial steering mechanism that responds to extrinsic cues during the initiation of cardiac development.

show abstract

Using Live Imaging to Examine Early Cardiac Development in Zebrafish

Cited by 3 publications

References 30 publications

The myocardium utilizes Pdgfra-PI3K signaling to steer towards the midline during heart tube formation

The myocardium utilizes Pdgfra-PI3K signaling to steer towards the midline during heart tube formation

Preclinical Models of Cardiac Disease: A Comprehensive Overview for Clinical Scientists

The myocardium utilizes a platelet-derived growth factor receptor alpha (Pdgfra)–phosphoinositide 3-kinase (PI3K) signaling cascade to steer toward the midline during zebrafish heart tube formation

Contact Info

Product

Resources

About