Using loofah reinforced chitosan-collagen hydrogel based scaffolds in-vitro and in-vivo; healing in cartilage tissue defects

Baysan, Gizem; Gunes, Oylum Colpankan; Turemis, Cihangir; Akokay Yilmaz, Pınar; Husemoglu, R. Bugra; Kara Ozenler, Aylin; Perpelek, Merve; Albayrak, Aylin Ziylan; Havitcioglu, Hasan; Cecen, Berivan

doi:10.1016/j.mtla.2023.101881

Cited by 5 publications

(1 citation statement)

References 41 publications

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“…On the other hand, as the nanofibrous structure, PHBV was used as it has good spinnability under wet-electrospinning conditions as well as it is biocompatible and biodegradable [34]. In our previous studies, wet electrospun PHBV nanofibers were used together with the macro-fibrous loofah mat in the design of scaffolds for both meniscus and osteochondral tissue regeneration [13,32]. These fibrous structures not only reinforced the hydrogels but also, they became favorable sites for cell attachment.…”

Section: Fabrication Of Lcpg Lcpg-h and Lcpg-e Scaffoldsmentioning

confidence: 99%

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

Baysan,

Yilmaz,

Albayrak

et al. 2024

Polym. Bull.

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In recent years, people have had more expectations from the developed technology in medicine, especially in the field of orthopedics and traumatology. Tissue engineers are interested in techniques that benefit from patients’ cells and biomaterials, instead of prostheses and implants. On the other hand, researchers have begun to use various medicinal plants for regeneration and anti-cancer studies. In the present study, we aimed to produce cartilage and bone inductive scaffolds for osteochondral tissue engineering applications with the addition of hawthorn or elderberry extracts. Firstly, wet electro-spun poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) fibers were integrated with a loofah mat. Then, they were impregnated into chitosan solution with/without hawthorn or elderberry extract. Composite hydrogel scaffolds were obtained by cross-linking with 0.3% (w/v) genipin. Fabricated scaffolds had more than 90% porosity and showed swelling capacity in the range of 1500–2200%. Based on the in vitro biocompatibility analyses using mesenchymal stem cells (MSCs), all the fabricated scaffolds were found to be biocompatible by WST-1, ALP activity, and GAG content analysis. Also, histological/immunohistochemical analyses showed that hawthorn and elderberry extract addition increased MSCs proliferation and collagen type I and II positivity. Consequently, all the scaffolds showed promising features for osteochondral tissue engineering applications. Graphical abstract

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Section: Fabrication Of Lcpg Lcpg-h and Lcpg-e Scaffoldsmentioning

confidence: 99%

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

Baysan,

Yilmaz,

Albayrak

et al. 2024

Polym. Bull.

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A comprehensive review on hydrogel-based bio-ink development for tissue engineering scaffolds using 3D printing

Gogoi,

Kumar,

Singh

2024

Annals of 3D Printed Medicine

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Raising the Bar: Progress in 3D-Printed Hybrid Bone Scaffolds for Clinical Applications: A Review

Khan,

Grewal,

Jun

et al. 2024

Cell Transplant

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Damage to bones resulting from trauma and tumors poses a significant challenge to human health. Consequently, current research in bone damage healing centers on developing three-dimensional (3D) scaffolding materials that facilitate and enhance the regeneration of fractured bone tissues. In this context, the careful selection of materials and preparation processes is essential for creating demanding scaffolds for bone tissue engineering. This is done to optimize the regeneration of fractured bones. This study comprehensively analyses the latest scientific advancements and difficulties in developing scaffolds for bone tissue creation. Initially, we clarified the composition and process by which bone tissue repairs itself. The review summarizes the primary uses of materials, both inorganic and organic, in scaffolds for bone tissue engineering. In addition, we present a comprehensive study of the most recent advancements in the mainstream techniques used to prepare scaffolds for bone tissue engineering. We also examine the distinct advantages of each method in great detail. This article thoroughly examines potential paths and obstacles in bone tissue engineering scaffolds for clinical applications.

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Using loofah reinforced chitosan-collagen hydrogel based scaffolds in-vitro and in-vivo; healing in cartilage tissue defects

Cited by 5 publications

References 41 publications

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

Loofah and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nano-fiber-reinforced chitosan hydrogel composite scaffolds with elderberry (Sambucus nigra) and hawthorn (Crataegus oxyacantha) extracts as additives for osteochondral tissue engineering applications

A comprehensive review on hydrogel-based bio-ink development for tissue engineering scaffolds using 3D printing

Raising the Bar: Progress in 3D-Printed Hybrid Bone Scaffolds for Clinical Applications: A Review

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