Using markers and field evaluation to identify the source of eyespot resistance gene<i>Pch1</i>in the collection of wheat breeding lines

Kwiatek, Michał; Wiśniewska, Halina; Kaczmarek, Z.; Korbas, M.; Gawłowska, Magdalena; Majka, Maciej; Pankiewicz, Katarzyna; Danielewicz, Jakub; Belter, Jolanta

doi:10.1556/0806.43.2015.024

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…Resistance expression was evaluated using inoculation tests made on 100 randomly chosen plants from each of four replications by calculating the infection index (K) and the percentage of infected stems according to Kwiatek et al (2012;2015). A sample of 50 plants from each replicate of each wheat genotype and the control varieties were evaluated (a total of 200 stems for each genotype).…”

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confidence: 99%

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Kwiatek

Wiśniewska

Korbas

et al. 2016

Journal of Plant Protection Research

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Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.

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confidence: 99%

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Kwiatek

Wiśniewska

Korbas

et al. 2016

Journal of Plant Protection Research

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“…Therefore, in this study, we used two more recent markers Xorw1 and Xorw5, which are more accurate in identifying the presence of a Pch1 gene because they are completely linked to Ep-D1 and Pch1 (Leonard et al 2008). Effectiveness of the markers selected for the identification of the Pch1 gene in winter wheat varieties in the presented study had already been reported by other researchers as well as in our previous research (Groenewald et al 2003;Santra et al 2006;Kwiatek et al 2012;Burt et al 2014;Kwiatek et al 2015Kwiatek et al , 2016.…”

Section: Resultsmentioning

confidence: 54%

Journal of Plant Protection Research

Majka¹,

Kwiatek²,

Korbas³

et al. 2018

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Eyespot is one of the most important fungal diseases of the stem base of wheat (Triticum aestivum L.). The presented study clearly demonstrated that the Pch1 gene was the main effective source for reducing the eyespot disease score in the analyzed winter wheat lines. Nevertheless, Pch1 was present only in 8−9% of the investigated lines. Using an isoenzymatic marker and molecular markers, the presence of the Pch1 gene and lack of the Pch2 gene was identified in six lines. Two lines, SMH 9409 and DL 358/13/4, were polymorphic in an isoenzymatic marker study. In the remaining three lines, C 3373/11-1, KBH 15.15 and KBP 1416, the Pch1 gene was identified only with the use of an isoenzymatic marker. Both genes Pch1 and Pch2, as well as the resistant variety Rendezvous, were found in three lines: DD 248/12, KBP 15.2 and STH 4431. In line DD 708/13, the presence of the Pch1 and Pch2 genes was identified, where the association between the Pch1 and the locus of the Xorw5 marker was broken. It was shown that the presence or absence of Pch1 and Pch2 genes did not significantly affect the grain yield (from the plot), although the yield was highest in the presence of both genes. A significant effect of the presence of the Pch1 gene on thousand kernel weight (TKW) was observed. Lines with the Pch1 gene showed significantly higher TKW values than lines without both genes or with the Pch2 gene only.

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Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection

Wiśniewska

Majka

Kwiatek

et al. 2019

Electronic Journal of Biotechnology

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Using markers and field evaluation to identify the source of eyespot resistance genePch1in the collection of wheat breeding lines

Cited by 4 publications

References 26 publications

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Journal of Plant Protection Research

Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection

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