Using microalgal communities for high CO2-tolerant strain selection

Wang, Hualong; Nche-Fambo, Fru Azinwi; Yu, Zhigang; Chen, Feng

doi:10.1016/j.algal.2018.08.038

Cited by 22 publications

(6 citation statements)

References 37 publications

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“…The particular step was followed by centrifugation at 4000 rpm, and the supernatant was collected. The phenol-sulfuric method was applied to determine the total content of carbohydrates in biomass [49]. The calibration plot was drawn at different glucose concentrations (0-0.1 mg mL −1 ).…”

Section: Analysis Of Total Carbohydrate (Cho) Contentmentioning

confidence: 99%

Bio-Mitigation of Carbon Dioxide Using Desmodesmus sp. in the Custom-Designed Pilot-Scale Loop Photobioreactor

et al. 2021

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Today’s society is faced with many upfront challenges such as the energy crisis, water pollution, air pollution, and global warming. The greenhouse gases (GHGs) responsible for global warming include carbon dioxide (CO2), methane (CH4), nitrous oxide (NOx), water vapor (H2O), and fluorinated gases. A fraction of the increased emissions of CO2 in the atmosphere is due to agricultural and municipal solid waste (MSW) management systems. There is a need for a sustainable solution which can degrade the pollutants and provide a technology-based solution. Hence, the present work deals with the custom design of a loop photobioreactor with 34 L of total volume used to handle different inlet CO2 concentrations (0.03%, 5%, and 10% (v/v)). The obtained values of biomass productivity and CO2 fixation rate include 0.185 ± 0.004 g L−1 d−1 and 0.333 ± 0.004 g L−1 d−1, respectively, at 10% (v/v) CO2 concentration and 0.084 ± 0.003 g L−1 d−1 and 0.155 ± 0.003 g L−1 d−1, respectively, at 5% (v/v) CO2 concentration. The biochemical compositions, such as carbohydrate, proteins, and lipid content, were estimated in the algal biomass produced from CO2 mitigation studies. The maximum carbohydrate, proteins, and lipid content were obtained as 20.7 ± 2.4%, 32.2 ± 2.5%, and 42 ± 1.0%, respectively, at 10% (v/v) CO2 concentration. Chlorophyll (Chl) a and b were determined in algal biomass as an algal physiological response. The results obtained in the present study are compared with the previous studies reported in the literature, which indicated the feasibility of the scale-up of the process for the source reduction of CO2 generated from waste management systems without significant change in productivity. The present work emphasizes the cross-disciplinary approach for the development of bio-mitigation of CO2 in the loop photobioreactor.

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Section: Analysis Of Total Carbohydrate (Cho) Contentmentioning

confidence: 99%

Bio-Mitigation of Carbon Dioxide Using Desmodesmus sp. in the Custom-Designed Pilot-Scale Loop Photobioreactor

et al. 2021

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“…Microalgae are considered a potential large-scale sink for CO 2 sequestration from flue gases, which contain 12-15% CO 2 [56,57]. However, most of the microalgal strains can only survive up to 5% CO 2 , hence development of high tolerance CO 2 strains is crucial [100][101][102]. Nuclear radiation ( 60 Co) was utilized to enhance the CO 2 tolerance of C. pyrenoidosa and C. vulgaris [74].…”

Section: Microalga Mutantsmentioning

confidence: 99%

Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts

2020

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Oleaginous microalgae and yeasts represent promising candidates for large-scale production of lipids, which can be utilized for production of drop-in biofuels, nutraceuticals, pigments, and cosmetics. However, low lipid productivity and costly downstream processing continue to hamper the commercial deployment of oleaginous microorganisms. Strain improvement can play an essential role in the development of such industrial microorganisms by increasing lipid production and hence reducing production costs. The main means of strain improvement are random mutagenesis, adaptive laboratory evolution (ALE), and rational genetic engineering. Among these, random mutagenesis and ALE are straight forward, low-cost, and do not require thorough knowledge of the microorganism’s genetic composition. This paper reviews available mutagenesis and ALE techniques and screening methods to effectively select for oleaginous microalgae and yeasts with enhanced lipid yield and understand the alterations caused to metabolic pathways, which could subsequently serve as the basis for further targeted genetic engineering.

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“…2 Therefore, the use of microalgae to reduce carbon emissions is a green, efficient, and recyclable carbon capture, utilization, and sequestration technology that is very promising. 3 Because they are photoautotrophs, the growth of microalgae for carbon sequestration is greatly influenced by changes in light irradiation, which is a key environmental factor for microalgae. While algal cells typically absorb all radiation in the visible spectrum, only a fraction of it can be used for photosynthesis.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, microalgae are photosynthetic autotrophs, which are endowed with many significant advantages such as high photosynthetic efficiency, short growth cycle, and strong environmental adaptability . Therefore, the use of microalgae to reduce carbon emissions is a green, efficient, and recyclable carbon capture, utilization, and sequestration technology that is very promising . Because they are photoautotrophs, the growth of microalgae for carbon sequestration is greatly influenced by changes in light irradiation, which is a key environmental factor for microalgae.…”

Section: Introductionmentioning

confidence: 99%

Photoautotrophic Growth and Cell Division of Euglena gracilis with Mixed Red and Blue Wavelengths

Xin,

Guo,

Wang

et al. 2024

Ind. Eng. Chem. Res.

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To improve flue gas CO 2 fixation of Euglena gracilis under a photoautotrophic environment, mixed red and blue wavelengths were used to promote photosynthetic growth and cell division with 15% CO 2 . The biomass dry weight increased by 385% to 1.31 g/L with a peak ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme activity and oxygen release, when the ratio of red to blue wavelength decreased to 2:3. E. gracilis mainly engaged in photochemical reactions with accumulated photosynthetic pigment to focus on light conversion by optimizing the carotenoid/chlorophyll ratio and chlorophyll a/b ratio. The energy capture efficiency of open photosystem II (PSII) reaction centers and quantum efficiency of charge separation in PSII reaction centers were improved to maintain active photosynthesis. Blue light facilitated transition from G1 to S phase, while red light promoted transition from S to G2 phase. The malondialdehyde content decreased to a valley of 1.36 nmol/mg protein to keep efficient photosynthetic apparatus with carbon fixation.

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Using microalgal communities for high CO2-tolerant strain selection

Cited by 22 publications

References 37 publications

Bio-Mitigation of Carbon Dioxide Using Desmodesmus sp. in the Custom-Designed Pilot-Scale Loop Photobioreactor

Bio-Mitigation of Carbon Dioxide Using Desmodesmus sp. in the Custom-Designed Pilot-Scale Loop Photobioreactor

Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts

Photoautotrophic Growth and Cell Division of Euglena gracilis with Mixed Red and Blue Wavelengths

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