For asphalt mixtures, it is widely known that Poisson's Ratio (PR) varies according to several parameters, and is a temperature and loading frequency-dependent property. However, measuring PR at different temperatures and frequencies during mechanical tests is not commonly done. A complex arrangement of fixtures and instruments are typically necessary, and are not compatible with typical cylindrical specimens. This work aimed to combine the simple and practical Ultrasonic Pulse Velocity (UPV) test with the conventional Dynamic Modulus (E*) test used for determining pavement design inputs related to asphalt mixtures. To do this, four typical lower binder asphalt concrete mixes were selected, having different levels of Asphalt Binder Replacement (ABR) given the presence of RAP/RAS, and covering seven different asphalt binders in terms of Performance Grade (PG). Additionally, a baseline mixture with no recycled material was used for comparison. Results show that estimating E* via UPV assuming a typical PR value may be inaccurate. Also, an increase in PR was observed with the increase in temperature, and reduction in ABR, as well as air voids. The findings indicate that PR can be a source of significant variability when it comes to the use of recycled materials, and that UPV is a relatively low-cost, simple, practical, and accurate piece of equipment with potential use as a complementary test to be carried out with E* for more accurate determination of PR, especially when comparing across different mixes.