SUMMARY:LSTVs are common within the spine, and their association with low back pain has been debated in the literature for nearly a century. LSTVs include sacralization of the lowest lumbar vertebral body and lumbarization of the uppermost sacral segment. These vertebral bodies demonstrate varying morphology, ranging from broadened transverse processes to complete fusion. Low back pain associated with an LSTV may arise from the level above the transition, the contralateral facet when unilateral, and/or the anomalous articulation when present. Although this association is still somewhat controversial, beyond dispute is the importance of identifying an LSTV in patients in whom a surgical or interventional procedure is planned. This is essential to avoid an intervention or surgery at an incorrect level. In this article, each of these issues will be addressed with attention to identifying and correctly numbering LSTVs as well as detecting imaging findings related to the genesis of low back pain.ABBREVIATIONS: AP ϭ anteroposterior; LSTV ϭ lumbosacral transitional vertebra L STVs are congenital spinal anomalies defined as either sacralization of the lowest lumbar segment or lumbarization of the most superior sacral segment of the spine. LSTVs are common in the general population, with a reported prevalence of 4%-30%.1-15 The degree of morphologic variation of these segments ranges from L5 vertebrae with broadened elongated transverse processes to complete fusion to the sacrum. Conversely, the S1 vertebral segment can show varying degrees of lumbarization, such as the formation of an anomalous articulation rather than fusion to the remainder of the sacrum, well-formed lumbar-type facet joints, a more squared appearance in the sagittal plane, as well as a well-formed fully-sized disk, rather than the smallersized disk typically seen between S1 and S2.