Using Nanomaterials as Excellent Immobilisation Layer for Biosensor Design

Idris, Azeez O.; Akanji, Seyi Philemon; Orimolade, Benjamin O.; Olorundare, Foluke O. G.; Azizi, Shohreh; Mamba, Bhekie B.; Mâaza, M.

doi:10.3390/bios13020192

Cited by 12 publications

(5 citation statements)

References 130 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PAMAM has also been used to create dendrimer-modified enzyme biosensors [ 31 ], DNA biosensors [ 32 ], immunosensors [ 33 ], and chemical sensors [ 34 ]. Nanomaterials with large surface areas and carefully spaced functional sites on their surfaces have been suggested to increase sensitivity, target molecule accessibility, provide quicker mass transfer rates, and shorten biosensor reaction times [ 35 ]. Electrospun nanofibers (ESNFs) can be considered suitable for supporting the immobilization of biorecognition elements because they meet several requirements, including maximal contact with the surrounding media, an extensively large surface area, a very porous structure, excellent surface modification, and subcellular size [ 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

et al. 2023

View full text Add to dashboard Cite

Herein, dendrimer-modified montmorillonite (Mt)-decorated poly-Ɛ-caprolactone (PCL) and chitosan (CHIT)-based nanofibers were prepared. Mt was modified with a poly(amidoamine) generation 1 (PAMAMG1) dendrimer, and the obtained PAMAMG1–Mt was incorporated into the PCL–CHIT nanofiber’s structure. The PCL–CHIT/PAMAMG1–Mt nanofibers were conjugated with glutamate oxidase (GluOx) to design a bio-based detection system for monosodium glutamate (MSG). PAMAMG1–Mt was added to the PCL–CHIT backbone to provide a multipoint binding side to immobilize GluOx via covalent bonds. After the characterization of PCL–CHIT/PAMAMG1–Mt/GluOx, it was calibrated for MSG. The linear ranges were determined from 0.025 to 0.25 mM MSG using PCL–CHIT/Mt/GluOx and from 0.0025 to 0.175 mM MSG using PCL–CHIT/PAMAMG1–Mt/GluOx (with a detection limit of 7.019 µM for PCL–CHIT/Mt/GluOx and 1.045 µM for PCL–CHIT/PAMAMG1–Mt/GluOx). Finally, PCL–CHIT/PAMAMG1–Mt/GluOx was applied to analyze MSG content in tomato soup without interfering with the sample matrix, giving a recovery percentage of 103.125%. Hence, the nanofiber modification with dendrimer-intercalated Mt and GluOx conjugation onto the formed nanocomposite structures was performed, and the PCL–CHIT/PAMAMG1–Mt/GluOx system was successfully developed for MSG detection.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Their careful control over size, shape, and surface qualities improves enzyme immobilization, stability, and selectivity, resulting in extremely sensitive and selective biosensing platforms. By enabling the quick, accurate, and accurate detection of analytes in complicated samples, the incorporation of nanomaterials in enzyme-based biosensors offers considerable potential for a variety of applications, including health care diagnosis, environmental monitoring, and food safety analyses [ 70 , 71 , 72 ]. As a result, a wide range of materials, such as carbon NMs, 2D materials, metal NPs, metal oxides, complexes, polymers, ionic liquids (ILs), and so on, are constantly being investigated.…”

Section: Nanomaterials That Are Generally Used In the Design Of Enzym...mentioning

confidence: 99%

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Kilic,

Singh,

Keles

et al. 2023

Biosensors

View full text Add to dashboard Cite

Electrochemistry is a genuinely interdisciplinary science that may be used in various physical, chemical, and biological domains. Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare applications, such as for the determination of glucose, lactate, catecholamines, nucleic acid, uric acid, and so on. Enzyme-based analytical techniques rely on detecting the co-substrate or, more precisely, the products of a catalyzed reaction. The glucose oxidase enzyme is generally used in enzyme-based biosensors to measure glucose in tears, blood, etc. Moreover, among all nanomaterials, carbon-based nanomaterials have generally been utilized thanks to the unique properties of carbon. The sensitivity can be up to pM levels using enzyme-based nanobiosensor, and these sensors are very selective, as all enzymes are specific for their substrates. Furthermore, enzyme-based biosensors frequently have fast reaction times, allowing for real-time monitoring and analyses. These biosensors, however, have several drawbacks. Changes in temperature, pH, and other environmental factors can influence the stability and activity of the enzymes, affecting the reliability and repeatability of the readings. Additionally, the cost of the enzymes and their immobilization onto appropriate transducer surfaces might be prohibitively expensive, impeding the large-scale commercialization and widespread use of biosensors. This review discusses the design, detection, and immobilization techniques for enzyme-based electrochemical nanobiosensors, and recent applications in enzyme-based electrochemical studies are evaluated and tabulated.

show abstract

“…However, biomolecule-assisted electrochemical biosensors for neurotransmitter detection are limited in terms of sensitivity and selectivity, owing to the low redox reaction efficiency and increased noise response. To address these problems, nanomaterials and nanotechnologies are frequently incorporated into electrochemical biosensors to improve their sensitivity and selectivity characteristics via (among others) the provision of large surface areas, an excellent template, and enhanced electron transfer [ 19 , 20 ]. Based on these studies, biosensors—which can accurately detect neurotransmitter changes without cells—or highly sensitive electrochemical biosensors—which can directly measure neurotransmitters released from cells—are being developed.…”

Section: Introductionmentioning

confidence: 99%

An Updated Review on Electrochemical Nanobiosensors for Neurotransmitter Detection

Choi,

Yoon

2023

Biosensors

View full text Add to dashboard Cite

Neurotransmitters are chemical compounds released by nerve cells, including neurons, astrocytes, and oligodendrocytes, that play an essential role in the transmission of signals in living organisms, particularly in the central nervous system, and they also perform roles in realizing the function and maintaining the state of each organ in the body. The dysregulation of neurotransmitters can cause neurological disorders. This highlights the significance of precise neurotransmitter monitoring to allow early diagnosis and treatment. This review provides a complete multidisciplinary examination of electrochemical biosensors integrating nanomaterials and nanotechnologies in order to achieve the accurate detection and monitoring of neurotransmitters. We introduce extensively researched neurotransmitters and their respective functions in biological beings. Subsequently, electrochemical biosensors are classified based on methodologies employed for direct detection, encompassing the recently documented cell-based electrochemical monitoring systems. These methods involve the detection of neurotransmitters in neuronal cells in vitro, the identification of neurotransmitters emitted by stem cells, and the in vivo monitoring of neurotransmitters. The incorporation of nanomaterials and nanotechnologies into electrochemical biosensors has the potential to assist in the timely detection and management of neurological disorders. This study provides significant insights for researchers and clinicians regarding precise neurotransmitter monitoring and its implications regarding numerous biological applications.

show abstract

Using Nanomaterials as Excellent Immobilisation Layer for Biosensor Design

Abstract: The progress in the field of material chemistry and nanotechnology in recent times has paved the way for remarkable accomplishments in development [...]

Cited by 12 publications

References 130 publications

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic–Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

An Updated Review on Electrochemical Nanobiosensors for Neurotransmitter Detection

Contact Info

Product

Resources

About