Field experiments were conducted at private farm at Sohag, Egypt, during the two successive summer seasons of 2018 and 2019, to study the response of sweet potato plants to soil-applied of organic manure (at rates of 0, 5, 10, 15 and 20 ton/fed.) and bio-fertilizer application (without or with Azotobater sp.) on growth, yield parameters and damage rates due to insect infestations. The obtained results indicated that, organic manure at rate 20 ton/fed was recorded the maximum increase in vegetative parameters i.e, the main stem length, No. of branches/plant, weight and dry matter% of vines, yield and its contents i.e., No., weight of storage roots/plant and dry matter percentage of storage roots compared with rate zero ton/fed., organic manure. Also, the same treatment gave significant increases in storage roots of N%, P%, K%, protein% and damage rates in leaves and roots as compared with other treatments in two seasons.Data assured that all studied parameters were affected significantly by adding Azotobater sp. expect, No. of branches and roots/plant, percentage of dry matter in branches, P, K, damage rates of leaves and roots in both seasons.The rate of 20 ton/fed (organic manure) with bio-fertilizer gave the highest values of the most studied characters except, total yield in the second season compared other treatments. While, the treatments zero ton/fed., organic manure without bio-fertilizer gave lowest values at all studied traits in both seasons.