Using Nuclear-encoded LSU and SSU rDNA Sequences to Identify the Eukaryotic Endosymbiont in Amphisolenia bidentata (Dinophyceae)

Daugbjerg, Niels; Jensen, Maria Hastrup; Hansen, Per Juel

doi:10.1016/j.protis.2012.10.001

Cited by 17 publications

(11 citation statements)

References 29 publications

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“…These strains are also related to the endosymbiont of the tropical dinoflagellate Amphisolenia bidentata (Daugbjerg et al. ). Strain RCC4552, isolated from the temperate North Atlantic, had a sequence only 98.7% similar to any other GenBank sequence and probably corresponds to a novel species.…”

Section: Discussionmentioning

confidence: 99%

Improvement of phytoplankton culture isolation using single cell sorting by flow cytometry

Marie

Gall

Edern

et al. 2017

Journal of Phycology

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Section: Discussionmentioning

confidence: 99%

Improvement of phytoplankton culture isolation using single cell sorting by flow cytometry

Marie

Gall

Edern

et al. 2017

Journal of Phycology

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“…(Farnelid et al 2010 ). The identity of the eukaryotic microalgae (approximately 100 cells located in the cytoplasm) has been recently revealed by 18S and 28S rRNA sequences as closely related to members of the Pelagophyceae (stramenopiles), presumably representing a new genus (Daugbjerg et al 2013 ).…”

Section: Dinofl Agellate Hostsmentioning

confidence: 99%

Photosymbiosis in Marine Planktonic Protists

2015

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Some of the most enigmatic components in the plankton are the diverse eukaryotic protists that live in close association with one or more partners. Mutualistic and commensal planktonic interactions are most commonly encountered in the oligotrophic open ocean at tropical and subtropical latitudes. They are functionally and ecologically distinct, and involve a great taxonomic diversity of single-celled partners. Protists like Foraminifera, Radiolaria, dinofl agellates and diatoms can all harbor microalgal symbionts of eukaryotic and prokaryotic origin inside (endosymbiosis) and/or outside (ectosymbiosis) their cytoplasm. Such symbioses (photosymbioses) combine phototrophy, heterotrophy and sometimes dinitrogen (N 2 ) fi xation (reduction of N 2 to ammonium). Symbiotic microorganisms therefore represent an important component of marine ecosystems and play a role in the food web and biogeochemical cycling (e.g., carbon and nitrogen). Despite their important ecological function and early recognition in the late nineteenth century, our knowledge about the diversity, distribution, and metabolic exchanges for many of the photosymbioses remains rudimentary compared with the other marine and terrestrial symbioses. Recent technical advances in single-cell genomics and imaging have greatly improved our understanding about planktonic symbioses. This review aims to present and compare many eukaryote-eukaryote and eukaryote-prokaryote photosymbioses described so far in the open ocean with an emphasis on their ecology and potential function in the ecosystem.

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“…D3A, D3B and D2CR). See Daugbjerg et al (2013) and Scholin et al (1994) for primer sequences. For sequencing we used the service provided by Macrogen, Korea.…”

Section: Dna Extraction and Pcr Amplification Of Lsu Rdnamentioning

confidence: 99%

Studies on woloszynskioid dinoflagellates VI: description ofTovellia aveirensissp. nov. (Dinophyceae), a new species of Tovelliaceae with spiny cysts

Pandeirada

Craveiro

Daugbjerg

et al. 2014

European Journal of Phycology

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A new species of Tovellia, T. aveirensis, is described on the basis of light (LM) and scanning electron microscopy (SEM) of motile cells and resting cysts, complemented with transmission electron microscopy (TEM) of flagellate cells and phylogenetic analysis of partial sequences of the large subunit ribosomal rRNA gene. Both vegetative cells and several stages of a life cycle involving sexual reproduction and the production of resting cysts were examined in cultures established from a tank in the University of Aveiro campus. Vegetative cells were round and little compressed dorsoventrally; planozygotes were longer and had a proportionally larger epicone. Chloroplast lobes were shown by TEM to radiate from a central, branched pyrenoid, although this was difficult to ascertain in LM. The amphiesma of flagellate cells had mainly 5 or 6-sided vesicles with thin plates, arranged in 5-7 latitudinal series on the epicone, 3-5 on the hypocone. The cingulum had 2 rows of plates, the posterior row extending into the hypocone and crossed by a series of small projecting knobs along the lower edge of the cingulum. A line of narrow amphiesmal plates extended over the cell apex, from near the cingulum on the ventral side to the middle of the dorsal side of the epicone. Eight or 9 narrow amphiesmal plates lined each side of this apical line of plates (ALP). Resting cysts differed from any described before in having numerous long, tapering spines with branched tips distributed over most of the surface. Most mature cysts showed an equatorial constriction. Neither cysts nor motile cells were seen to accumulate red cytoplasmic bodies in any stage of the cultures. The phylogenetic analysis placed, with high statistical support, the new species within the genus Tovellia; it formed a clade, with moderate support, with T. sanguinea, a species notable for its reddening cells.

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Using Nuclear-encoded LSU and SSU rDNA Sequences to Identify the Eukaryotic Endosymbiont in Amphisolenia bidentata (Dinophyceae)

Cited by 17 publications

References 29 publications

Improvement of phytoplankton culture isolation using single cell sorting by flow cytometry

Improvement of phytoplankton culture isolation using single cell sorting by flow cytometry

Photosymbiosis in Marine Planktonic Protists

Studies on woloszynskioid dinoflagellates VI: description ofTovellia aveirensissp. nov. (Dinophyceae), a new species of Tovelliaceae with spiny cysts

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