Using of Essential Oils and Plant Extracts against Pseudomonas savastanoi pv. glycinea and Curtobacterium flaccumfaciens pv. flaccumfaciens on Soybean

Abstract

The bacteria Pseudomonas savastanoi pv. glycinea (Coerper, 1919; Gardan et al., 1992) (Psg) and Curtobacterium flaccumfaciens pv. flaccumfaciens (Hedges 1922) (Cff) are harmful pathogens of soybean (Glycine max). Presently, there are several strategies to control these bacteria, and the usage of environmentally friendly approaches is encouraged. In this work, purified essential oils (EOs) from 19 plant species and total aqueous and ethanolic plant extracts (PEs) from 19 plant species were tested in vitro to observe their antimicrobial activity against Psg and Cff (by agar diffusion and broth microdilution method). Tested EOs and PEs produced significant bacterial growth inhibition with technologically acceptable MIC and MBC values. Non-phytotoxic concentrations for Chinese cinnamon and Oregano essential oils and leather bergenia ethanolic extract, which previously showed the lowest MBC values, were determined. Testing of these substances with artificial infection of soybean plants has shown that the essential oils of Chinese cinnamon and oregano have the maximum efficiency against Psg and Cff. Treatment of leaves and seeds previously infected with phytopathogens with these essential oils showed that the biological effectiveness of leaf treatments was 80.6–77.5% and 86.9–54.6%, respectively, for Psg and Cff. GC-MS and GC-FID analyzes showed that the major compounds were 5-Methyl-3-methylenedihydro-2(3H)-furanone (20.32%) in leather bergenia ethanolic extract, cinnamaldehyde (84.25%) in Chinese cinnamon essential oil and carvacrol (62.32%) in oregano essential oil.

Antibacterial Effect of Juglans regia, Citrus sinensis, Vicia faba, and Urtica urens Extracts under In vitro Conditions

“…This experiment aims to control or treat plant diseases such as soft rot and bacterial wilt. Antibiotics and fungi pesticides such as captan, streptomycin, difenoconazole and streptocycline are applied to prevent or treat diseases caused by P. carotovorum, R. solanacearum, D. chrysanthemi and P. syringae pathogens, respectively [17]- [20]. However, anti-biotic resistance has become a worldwide environmental challenge and also some anti-biotics are also known to have undesirable effects on chloroplast and nuclear gene expression [21] [22].…”

Development of a multiplex real-time PCR method for the detection of Pseudomonas savastanoi pv. glycinea and Curtobacterium flaccumfaciens pv. flaccumfaciens in soybean seeds

“…The control of soybean bacterial diseases mostly relies on prevention of the infection. Some positive results have also been achieved through the use of resistant cultivars (Monteil et al, 2016;Silva Júnior et al, 2012;Urrea and Harveson, 2014), chemical bactericides including copper compounds (Estefani et al, 2007), nanoparticles of chitosan with copper (Tarakanov et al, 2023), antagonistic bacteria (Völksch and May, 2001), Plant-Growth Promoting Rhizobacteria (PGPR) (Martins et al, 2013), bacteriophages (Tarakanov et al, 2022b, c) and essential oils and plant extracts (Rani et al, 2008;Tarakanov and Dzhalilov, 2022).…”

Microbial and Plant-Based Compounds as Alternatives for the Control of Phytopathogenic Bacteria

“…Moreover, combinations or synergistic effects of EOs, such as those derived from Thymus and Origanum species, have been found to exhibit antibacterial properties when applied undiluted, with higher susceptibility determined for Xanthomonas strains compared with Pseudomonas or Erwinia taxa [96]. Particularly low MIC values listed in Table 2, indicate high efficacy of Thymus and Origanum EOs as antimicrobials [96][97][98][99][100][101][102]. In the study by Bozkurt et al [98], as well as previous studies, the EOs derived from Thymus and Origanum species, which are primarily composed of thymol and carvacrol, demonstrated the strongest inhibition of bacterial growth in vitro.…”

“…Remarkably, no phytotoxicity was observed in any of the treatment groups, indicating the safety of G. biloba leaf extract as a natural pesticide. In the study by Tarakanov and Dzhalilov [100], two EOs obtained from Cinnamomum cassia (Chinese cinnamon) and O. vulgare (oregano), inhibited the growth of P. savastanoi pv. glycinea and Curtobacterium flaccumfaciens pv.…”