Several studies have reported the MEs content in EPF of normal and affected gland. [71][72][73][74][75][76][77][78] However, further investigation has been considered necessary to provide clearer hypothesis about the role of MEs in etiology and pathogenesis of prostate disorders, because the findings of various studies indicate some discrepancies. The present study addresses the significance of prostatic fluid Ca, Cl, K, Mg, and Na levels as biomarker. Therefore, we systematically reviewed the available literature and performed a statistical analysis of Ca, Cl, K, Mg, and Na concentration in EPF of normal gland, which may shed valuable insight into the etiology and diagnosis of prostate disorders.