Using Quantitative Real Time PCR Melt Curve Analysis of Partial CO1 Sequence for Rapid Biotype Differentiation of<i>Bactericera cockerelli</i>(Hemiptera: Triozidae)

Chapman, Rebekah I.; Macias-Velasco, Juan F; Arp, Alex P.; Bextine, Blake

doi:10.3958/059.037.0405

Cited by 16 publications

(14 citation statements)

References 27 publications

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“…Previous HRM analyses have utilized several CO1 amplicons of different sizes to differentiate between the three known psyllid haplotypes ( Chapman et al 2012 ; Swisher et al 2012 , 2013 ). Initial HRM analyses of psyllids collected in the southwestern United States during the 2012 growing season utilized a 353-bp CO1 amplicon.…”

Section: Resultsmentioning

confidence: 99%

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Identification of a Fourth Haplotype of Bactericera cockerelli (Hemiptera: Triozidae) in the United States

Swisher

Henne²

2014

Journal of Insect Science

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The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a pest of potato and other solanaceous crops in North and Central America and New Zealand. Previous genotyping studies have demonstrated the presence of three different haplotypes of B. cockerelli in the United States corresponding to three geographical regions: Central, Western, and Northwestern. These studies utilized psyllids collected in the western and central United States between 1998 and 2011. In an effort to further genotype potato psyllids collected in the 2012 growing season, a fourth B. cockerelli haplotype was discovered corresponding to the Southwestern United States geographical region. High-resolution melting analyses identified this new haplotype using an amplicon generated from a portion of the B. cockerelli mitochondrial cytochrome coxidase subunit I gene. Sequencing of this gene, as well as use of a restriction enzyme assay, confirmed the identification of the novel B. cockerelli haplotype in the United States.

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Section: Resultsmentioning

confidence: 99%

“…Species specificity has been previously determined for these primers ( Chapman et al 2012 , Swisher et al 2013 ). Briefly, primers CO1 F3 and CO1 meltR generate a 94-bp amplicon and were used in conjunction with primers CO1 meltF and CO1 meltR, which generate a 67-bp amplicon, for HRM analysis ( Chapman et al 2010 , Swisher et al 2013 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of a Fourth Haplotype of Bactericera cockerelli (Hemiptera: Triozidae) in the United States

Swisher

Henne²

2014

Journal of Insect Science

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“…Individual potato psyllid nucleic acid extractions were done using the CTAB (cetyltriethylammonium bromide) buffer method (Zhang et al 1998). After DNA extraction, all samples were checked with a Nanodrop 1000 (Thermo Fischer ScientiÞc Inc, Waltham, MA) and potato psyllid haplotype detection described by Chapman et al (2012) to ensure the quality of the DNA extract.…”

Section: Methodsmentioning

confidence: 99%

Low-Level Detection ofCandidatusLiberibacter Solanacearum inBactericera cockerelli(Hemiptera: Triozidae) by 16s rRna Pyrosequencing: Table 1.

2013

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Accurate detection and quantification of Candidatus Liberibacter solanacearum (CLs), the putative causal agent of zebra chip disease of potato (Solanum tuberosum L.), in the potato psyllid, Bactericera cockerelli (Sulc), has become necessary to better understand the biology of the disease cycle. Studies on the transmission efficiency of potato psyllids have shown inconsistencies with field surveys. There have also been reports of laboratory colonies inexplicably losing and regaining CLs infection as detected by polymerase chain reaction (PCR). Until now, DNA primers were used to detect CLs in potato psyllid tissue using conventional polymerase chain reaction (PCR) and gel electrophoresis or by real-time quantitative PCR. In this study, CLs was detected using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) at levels identifiable by PCR, and low levels, including samples with only one cell of CLs. Potato psyllids with <300 pyrosequencing reads did not show positive using conventional PCR. These results indicate that the currently accepted PCR diagnostic technique produces false negatives due to detection limits higher than what is generally present in field collected psyllids, and also provides an explanation as to why laboratory colonies seem to lose and regain CLs infection.

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“…Primers CO1 meltF and CO1 meltR also were tested for species speciÞcity against two carrot psyllids (Trioza apicalis Forster), and were not found to amplify (data not shown). In addition, primers CO1 meltF and CO1 meltR (termed BB bc melt CO1 forward and BB bc melt Reverse) were tested by Chapman et al (2012) for species speciÞcity against Myndus crudus Van Duzee, Diaphorina citri Kuwayama, Homalodisca vitripennis (Germar), and Solenopsis invicta Buren by using HRM analyses. These primers were found to be speciÞc to the potato psyllid.…”

Section: Origin Ofmentioning

confidence: 99%

“…This CO1 gene has been used for phylogeographical studies of the Asian citrus psyllid, Diaphorina citri Kuwayama, which also acts as a vector of Candidatus Liberibacter asiaticus, in the Americas and the PaciÞc (deLeó n et al 2011). Similarly, the CO1 gene has been used for three different phylogenetic studies of the potato psyllid (Liu et al 2006, Chapman et al 2012, Swisher et al 2012.…”

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confidence: 98%

Temporal and Spatial Analysis of Potato Psyllid Haplotypes in the United States

Swisher

Munyaneza

2013

Environ Entomol

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The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is an economically important pest of potato (Solanum tuberosum L.) crops across the western and central United States, as it is known to cause psyllid yellows disease and to transmit the bacterium that causes zebra chip disease. Recent genotyping of B. cockerelli collected during the 2011 potato growing season identified three psyllid haplotypes within the western and central United States according to their geographical regions: northwestern, western, and central. To understand potato psyllid population dynamics before the year 2011, high resolution melting analysis of the B. cockerelli mitochondrial cytochrome oxidase I-like gene was used to identify the haplotypes of over 450 archived psyllids collected in the western and central United States between the years 1998 and 2010. Results show that the northwestern haplotype was present in Washington State as early as 1998 and has persisted in this region since that time. Likewise, psyllids of the western haplotype have also been present in Washington and Oregon before 2011.

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Using Quantitative Real Time PCR Melt Curve Analysis of Partial CO1 Sequence for Rapid Biotype Differentiation ofBactericera cockerelli(Hemiptera: Triozidae)

Cited by 16 publications

References 27 publications

Identification of a Fourth Haplotype of Bactericera cockerelli (Hemiptera: Triozidae) in the United States

Identification of a Fourth Haplotype of Bactericera cockerelli (Hemiptera: Triozidae) in the United States

Low-Level Detection ofCandidatusLiberibacter Solanacearum inBactericera cockerelli(Hemiptera: Triozidae) by 16s rRna Pyrosequencing: Table 1.

Temporal and Spatial Analysis of Potato Psyllid Haplotypes in the United States

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