Using species-specific PCR technique to detect Toxoplasma gondii in broiler chickens

Al-Sanjary, Raad A.; Hussein, T. H.

doi:10.33899/ijvs.2012.67452

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…Toxo-DNA prevalence (10.84%.) of the current study is higher than reported from neighboring countries like India (6.06%) [70] and Iran (8%) [71], but lower than reported from Kenya (24-79%) [63,72], and Iraq (26.9%) [73]. The change in prevalence might be due to the differences in geography, climate, the number and age of chickens examined, and sanitation conditions [68].…”

Section: Plos Onecontrasting

confidence: 65%

Molecular identification of Toxoplasma gondii in domesticated and broiler chickens (Gallus domesticus) that possibly augment the pool of human toxoplasmosis

Khan

Rafiq

et al. 2020

PLoS ONE

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Toxoplasma gondii (T. gondii) is a protozoan parasite that infects all warm-blooded animals including domesticated birds and humans. Birds normally get infected by ground feeding and human beings contract the disease by consumption of undercooked chicken meat. This study aimed to analyze seroprevalence and DNA of T. gondii in chickens (domesticated and broiler) and to assess possible transfer to humans by review of available literature from Pakistan. Blood from and tissues from domesticated and broilers chickens were analyzed for Toxo-IgM/IgG and Toxoplasma DNA through ELISA and PCR respectively. Furthermore, research articles published during 1990-2019 on the prevalence of T. gondii in humans from Pakistan, were analyzed to assess the possible infection burden in the area in connection to transmission from chickens. The overall prevalence of IgM and IgG for T. gondii was 17.83% and 8.8% respectively in the study areas. Significant seroprevalence was found in domesticated chickens than broilers. In domesticated chickens, the prevalence was high in age � 2 years. Toxoplasma DNA was detected in tissues with an overall prevalence of 10.84%. Higher prevalence was observed in liver (10.50%) than heart (9.5%) and muscles (7.11%). Only 4.78% broiler and 2.38% domesticated chickens were positive for both IgM and DNA, 1.2% domesticated and 1.30% broilers were positive for IgG and DNA, while 2.98% domesticated and 2.17% broilers were positive for IgM, IgG, and DNA. Available literature showed that 25.8% of human beings were infected with T. gondii in Pakistan. The prevalence was 20.64% in male and 26.82%in the female. The rate of infections increases with age and high (37.36%) was found in humans of age range 40 to 60 years. A high prevalence of T. gondii is found in both domesticated and broiler chickens in the study area. Moreover, the literature survey indicates that a high seroprevalence of T. gondii is present in human beings of Pakistan. It is concluded that the high prevalence of T. gondii in humans may be associated with the parasite transmission through infected chicken's meat in Pakistan.

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Section: Plos Onecontrasting

confidence: 65%

Molecular identification of Toxoplasma gondii in domesticated and broiler chickens (Gallus domesticus) that possibly augment the pool of human toxoplasmosis

Khan

Rafiq

et al. 2020

PLoS ONE

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Section: Resultssupporting

confidence: 86%

The Role of K+ Channel Subtypes in Catechin Induced Vasorelaxation in Rat’s Aortic Rings

Al-Habib

Sabir

2015

SJUOZ

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The current study aimed to investigate the role of K + channels subtypes in relaxant effects of catechin on rat's aortic rings. The isometric tension of the thoracic aorta was measured using ADI PowerLab Data Acquisition System. Catechin at concentrations (10-5 to 5x10-1 M) produced more potent relaxant effects in phenylephrine (10-6 M) precontracted aortic smooth muscle with Log IC 50 's of-1.159 mg/ml as compared to potassium chloride (60 mM) with Log IC 50 's of-6.373 mg/ml. The catechin induced relaxation in aortic rings precontracted with phenylephrine was 17.464 ± 0.068 %, where as for aortic rings precontracted with potassium was only 5.574 ± 0.131%. In aortic rings preincubated with glibenclamide (10-5 M) and tetraethylammonium (1mM), catechin relaxant effect was enhanced with Log IC 50 s of-3.119 and-3.001 mg/ml, respectively. On the other hand, in aortic rings precontracted with PE and preincubated with BaCl 2 and 4-AP, catechin induced statistically non-significant relaxant effects as compared with aortic rings of the control aortic ring which was precontracted with PE. From the results of the current study, it can be concluded that catechin produced more potent vasorelaxant effect on aortic rings preincubated with phenylephrine that KCl. This vasorelaxant effect of catechin is produced via the activation of both K ATP and Kca, but not Kir and Kv channels subtypes.

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“…As the use of the medication throughout pregnancy resulted in improved clinical results. In summary, many zoonotic diseases exist (43)(44)(45)(46)(47); Toxoplasmosis still has many layers still to be unraveled.…”

Section: Toxoplasmosis Trend In Pregnancymentioning

confidence: 99%

Toxoplasmosis, a zoonotic infection; A critical and updated analysis: A Review Article

Nori¹,

Ali²

2021

IJVS

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Toxoplasmosis is a parasitic illness caused by Toxoplasma gondii, an obligate intracellular protozoan. It may infect humans and pets, as well as other warm-blooded mammals. Toxoplasmosis can be silent in the general public but it can be deadly in immunocompromised patients. This neurotropic protozoan has been linked to an increased risk of behavioral changes in infected animals and mental illnesses in human beings. The detection of a biological pathogen linked to schizophrenia is critical to comprehend the pathogen's biological influence on affected cases. Moreover, in schizophrenia cases, toxoplasmosis infection and lipid profile disturbance are frequent. The exact underlying pathology is yet unknown. Pregnant women are a particular risk group; they are liable to a higher risk of infection, leading to miscarriage, stillbirth, or permanent impairments in the unborn child. This review aimed to explore the up-to-date knowledge on this zoonotic parasite and critically analyze current information, inconsistencies, and implications on public health. We discuss the pathological pathways by which it produces its devastating effect on the central nervous system in both animals and human models, in addition to its enigmatic impact on lipid profile.

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Using species-specific PCR technique to detect Toxoplasma gondii in broiler chickens

Cited by 7 publications

References 11 publications

Molecular identification of Toxoplasma gondii in domesticated and broiler chickens (Gallus domesticus) that possibly augment the pool of human toxoplasmosis

Molecular identification of Toxoplasma gondii in domesticated and broiler chickens (Gallus domesticus) that possibly augment the pool of human toxoplasmosis

The Role of K+ Channel Subtypes in Catechin Induced Vasorelaxation in Rat’s Aortic Rings

Toxoplasmosis, a zoonotic infection; A critical and updated analysis: A Review Article

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