Using Start Codon Targeted (SCoT) polymorphism for Genetic Diversity Analysis of three Red Sea fishes (Family: Carangidae)

Marie, Zeinab A.; Allam, Mohammad

doi:10.9790/3008-1201045056

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…The second marker was SCoT marker which developed by Collard and Mackill (2009) for genetic analysis and generating gene-targeted markers in plants. However, SCoT was used recently to study genetic diversity in other organisms such as fish (Marie and Allam, 2017) and camel (Al-Soudy et al, 2018). In addition, it was also used as a novel gene targeting marker in sex-determination in date palm (Mohamed and Sami, 2015) with no available reports about using it in molecular sexing studies in animals.…”

Section: Discussionmentioning

confidence: 99%

Karyotyping and Molecular Sexing in Brycinus nurse (Characiformes, Alestidae) from Nile River in Egypt

Elsayed

Zohry

Gamal

et al. 2019

Journal of Agricultural Chemistry and Biotechnology

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The present study aimed to investigate the karyotype and molecular sex differences in Brycinus nurse. Analysis of 430 metaphases spreads from 12 specimens revealed that the diploid chromosome number is 44 chromosomes and the proposed karyotype formula is n = 3 M + 3 SM + 16 A, FN = 56. No morphologically differentiated heteromorphic sex chromosomes were observed. On the other hand, 29 primers of three molecular markers (RAPD, SCoT and ISSR) were tested to differentiate between males and females of B. nurse. From all tested primers only two primers (OPI-18 and SCoT-18) successfully generated female specific bands in the bulked DNA samples which further confirmed using individual DNA samples. According to molecular analysis result in this study it seems that females of B. nurse are the heterogametic gender (ZW) and males of B. nurse are the homogametic gender (ZZ). In the future, SCoT marker can be used as suitable and powerful marker in genetic analysis studies in fish.

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Section: Discussionmentioning

confidence: 99%

Karyotyping and Molecular Sexing in Brycinus nurse (Characiformes, Alestidae) from Nile River in Egypt

Elsayed

Zohry

Gamal

et al. 2019

Journal of Agricultural Chemistry and Biotechnology

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“…Both of these markers are widely used in studies of population genetics in plants. In animals, SCoT markers have also been used in studies of population genetics (Zeinab & Mohammad, 2017;Ali et al, 2020;Ali, 2020), but CBDP markers have not been used. In the gene structure in eukaryotes, the CAAT box is located upstream of the transcription initiation site and signals the binding site for the RNA transcription factor, namely NF-Y subunits.…”

Section: Introductionmentioning

confidence: 99%

Comparison between scot and CBDP techniques in assessment genetic diversity and variation of two populations of bigfin reef squid (<i>Sepioteuthis lessoniana</i> d’Orbigny) in Con Dao and Phu Quoc islands, Vietnam

Le,

Thai,

Nguyen

2024

AJB

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The Start Codon Targeted Polymorphism (SCoT) and CAAT box-derived polymorphism (CBDP) techniques were used to analyze the genetic diversity and variation of two bigfin reef squid populations in waters surrounding the Con Dao and Phu Quoc islands of Vietnam for technical comparison. The two used techniques reflected different levels of pairwise genetic similarity among individuals depended on the investigated population. Gene differentiation (GST) between the two investigated populations was 0.0767 and 0.0373 leaded to the genetic distance between them was 0.0381 and 0.0228, and the gene flow was Nm = 6.0195 and 12.9061 migrants per generation between the populations based on SCoT and CBDP techniques, respectively. Genetic variation within individuals of both populations (WP) played the key role in the total genetic variation of whole species in surveyed geographic regions with the distribution of 91.44% based on SCoT data and 93.76% based on CBDP data, the distribution of genetic variation among populations (AP) was small. For whole species in the surveyed region, the CBDP markers showed higher genetic diversity, while the SCoT markers reflected the differentiation and genetic distance between the two investigated populations better. Overall, the abilities to detect polymorphisms and the number of revealed loci using SCoT markers were better than using CBDP markers, while the ability to distinguish samples and the primer combination to detect the differences among investigated samples using CBDP markers were better than using SCoT markers, and the overall utility was comparable between these two marker systems. The results from this study prove that the CBDP technique can also be used in studies of animal population genetics.

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Using Start Codon Targeted (SCoT) polymorphism for Genetic Diversity Analysis of three Red Sea fishes (Family: Carangidae)

Cited by 2 publications

References 19 publications

Karyotyping and Molecular Sexing in Brycinus nurse (Characiformes, Alestidae) from Nile River in Egypt

Karyotyping and Molecular Sexing in Brycinus nurse (Characiformes, Alestidae) from Nile River in Egypt

Comparison between scot and CBDP techniques in assessment genetic diversity and variation of two populations of bigfin reef squid (<i>Sepioteuthis lessoniana</i> d’Orbigny) in Con Dao and Phu Quoc islands, Vietnam

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