Using sweeping micellar electrokinetic chromatography to analyze Δ9-tetrahydrocannabinol and its major metabolites

Su, Hsiu Li; Feng, Lan Ing; Jen, Hsiu Ping; Hsieh, You‐Zung

doi:10.1016/j.chroma.2008.10.004

Cited by 14 publications

(12 citation statements)

References 22 publications

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“…Several on‐line preconcentration methods have been developed to overcome the low detection sensivity of MEKC such as: sweeping stacking including field enhanced amplified sample stacking , dynamic pH junction , and transient ITP (tITP) . These techniques were applied to concentrate a wide range of neutral and charged analytes by injecting a larger sample amount and focusing it into a narrow band of the capillary .…”

Section: Improving Detection Sensitivitymentioning

confidence: 99%

Recent methodological and instrumental development in MEKC

2013

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The review gives an update about the methodological and instrumental developments in micellar electrokinetic capillary chromatography as a type of CE analytical technique. Here, the last two years development of the technique are particularly presented. Recent approaches to improve sensitivity are discussed. Newly introduced concentration techniques and experimental methods for verification of the different mechanisms and processes of micellar electrokinetic chromatography analysis are highlighted. A theoretical model to explain changes in separation and electrophoretic mobility order of fully charged analytes are demonstrated. Modern approaches for improving compatibility of micellar electrokinetic capillary chromatography to mass spectrometry are also reported.

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Section: Improving Detection Sensitivitymentioning

confidence: 99%

Recent methodological and instrumental development in MEKC

2013

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“…Su et al . 143 combined sweeping‐MEKC with SPE for human urine analysis of Δ9‐tetrahydrocannabinol and its metabolites (11‐hydroxy‐Δ9‐tetrahydrocannabinol and 11‐nor‐9‐carboxy‐Δ9‐tetrahydrocannbinol). The LODs of these compounds in urine samples ranged from 17.2 to 23.3 ng/mL.…”

Section: Sensitivity In Mekcmentioning

confidence: 99%

MEKC as a powerful growing analytical technique

2010

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This review summarizes the principle and the developments in MEKC in terms of separation power, sensitivity, and detection approaches more than 25 years after its appearance. Newly used surfactants are mentioned. Classical and new sample concentration techniques in MEKC are described. The different detection approaches in MEKC with advantages, limitations, and future prospects are also discussed. This review highlights the wider application of MEKC in different analytical fields. Various recent selected applications of this technique in different analytical fields are reported.

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“…The “classical” protocol for sweeping neutral and anionic analytes by using SDS and suppressing EOF in a low pH BGE provided by phosphoric acid or phosphate buffer (pH 2–3) has been widely used for improving sensitivity in many analytical determinations 29–36. In general, the enhancement factors ranged from ca .…”

Section: Approaches For Improving Sensitivitymentioning

confidence: 99%

Micellar electrokinetic chromatography: A practical overview of current methodological and instrumental advances

Silva

2010

Electrophoresis

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This review highlights recent methodological and instrumental advances in MEKC focused on the improvement of this CE technique in routine analyses. As in the previous reviews, the present one deals with the last 2 years' most relevant methodological contributions for improving sensitivity and resolution in MEKC as well as instrumental research related to MS and LIF detection. The most widespread approaches are discussed in detail, including enhancements in sensitivity arising from the use of on-line sample concentration (stacking, sweeping, and a combination of both of these protocols) and improvements in resolution, obtained by changing the composition of the BGE (e.g. with additives to the aqueous phase or employing alternative micellar phases to classical SDS) and using 2-D separation systems. The advantages and restrictions in MS and LIF detection as applied to MEKC analysis are also examined, especially in the direct coupling of MEKC and MS detection. Finally, some thoughts on potential future directions are also considered.

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Using sweeping micellar electrokinetic chromatography to analyze Δ9-tetrahydrocannabinol and its major metabolites

Cited by 14 publications

References 22 publications

Recent methodological and instrumental development in MEKC

Recent methodological and instrumental development in MEKC

MEKC as a powerful growing analytical technique

Micellar electrokinetic chromatography: A practical overview of current methodological and instrumental advances

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