Using Taguchi Method to Determine the Optimum Conditions for Synthesizing Parapyruvate

Lee, Inn; Yang, Nae-Cherng

doi:10.3390/molecules27061870

Cited by 6 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned above, the formation of citramalate might be the consequence of either a condensation reaction between the produced acetate and pyruvate or of a decarboxylation of the produced parapyruvate. Parapyruvate is a well-known and widely reported homo-aldol condensation product of pyruvate [84][85][86] , which is more favored under alkaline conditions 87 . To investigate the role of pH for pyruvate conversion to citramalate, two more parallel reactions were performed at pH 7 and 9 with the addition of KOH, and the outcomes are presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ambient temperature CO2 fixation to pyruvate and subsequently to citramalate over iron and nickel nanoparticles

et al. 2023

View full text Add to dashboard Cite

The chemical reactions that formed the building blocks of life at origins required catalysts, whereby the nature of those catalysts influenced the type of products that accumulated. Recent investigations have shown that at 100 °C awaruite, a Ni3Fe alloy that naturally occurs in serpentinizing systems, is an efficient catalyst for CO2 conversion to formate, acetate, and pyruvate. These products are identical with the intermediates and products of the acetyl-CoA pathway, the most ancient CO2 fixation pathway and the backbone of carbon metabolism in H2-dependent autotrophic microbes. Here, we show that Ni3Fe nanoparticles prepared via the hard-templating method catalyze the conversion of H2 and CO2 to formate, acetate and pyruvate at 25 °C under 25 bar. Furthermore, the 13C-labeled pyruvate can be further converted to acetate, parapyruvate, and citramalate over Ni, Fe, and Ni3Fe nanoparticles at room temperature within one hour. These findings strongly suggest that awaruite can catalyze both the formation of citramalate, the C5 product of pyruvate condensation with acetyl-CoA in microbial carbon metabolism, from pyruvate and the formation of pyruvate from CO2 at very moderate reaction conditions without organic catalysts. These results align well with theories for an autotrophic origin of microbial metabolism under hydrothermal vent conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Ambient temperature CO2 fixation to pyruvate and subsequently to citramalate over iron and nickel nanoparticles

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Previously, we have shown that parapyruvate is a reversibly competitive inhibitor of the KGDHC activity and can induce senescence in Hs68 cells by inhibiting the cellular KGDHC activity. 2,5 In this study, we administered parapyruvate to the C57BL/6JNarl mice at dosages ranging from 5 to 500 mg/kg/ day and observed a dose-dependent impairment of the learning and memory ability. Furthermore, we observed that parapyruvate both decreased the KGDHC activity and increased the oxidative stress in the brain in a dose-dependent manner, as evidenced by the decreased levels of GSH and the SOD activity and the increased levels of MDA and nitrite in the brain.…”

Section: Discussionmentioning

confidence: 94%

“…Parapyruvate is formed by the aldol addition reaction of two molecules of pyruvate and is commonly found in commercial dietary supplements of calcium pyruvate (DSCP). − Parapyruvate is a reversibly competitive inhibitor of the α-ketoglutarate dehydrogenase complex (KGDHC) with a structure similar to α-ketoglutarate (α-KG), which is the substrate of KGDHC . Parapyruvate can also be used as an inhibitor of the tricarboxylic acid cycle (TCA) .…”

Section: Introductionmentioning

confidence: 99%

“… 2 − 4 Parapyruvate is a reversibly competitive inhibitor of the α-ketoglutarate dehydrogenase complex (KGDHC) with a structure similar to α-ketoglutarate (α-KG), which is the substrate of KGDHC. 5 Parapyruvate can also be used as an inhibitor of the tricarboxylic acid cycle (TCA). 6 Previous studies have shown that the decreased KGDHC activity is significantly correlated with many neurodegenerative diseases, 7 , 8 such as Alzheimer’s disease (AD) 9 − 13 and Parkinson’s disease (PD).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Parapyruvate Induces Neurodegeneration in C57BL/6JNarl Mice via Inhibition of the α-Ketoglutarate Dehydrogenase Complex

Lee,

Song,

Chen

et al. 2024

ACS Omega

Self Cite

View full text Add to dashboard Cite

Parapyruvate is a substance commonly found in commercial dietary supplements of calcium pyruvate (DSCP) that inhibits the α-ketoglutarate dehydrogenase complex (KGDHC) and has been shown to induce senescence in human Hs68 cells. However, it is unknown whether parapyruvate can induce neurodegeneration. In this study, the parapyruvate content in DSCP was converted to an equivalent dose for mice and administered to the C57BL/6JNarl mice at doses around the equivalent dose for 69 days, including 5, 50, and 500 mg/kg/day. The Morris water maze (MWM) task and the active avoidance test were conducted to assess the learning and memory ability in mice, and then brain tissues were collected for biochemical analyses. The results demonstrated that parapyruvate significantly impaired the learning and memory ability, decreased the KGDHC activity, and promoted the oxidative stress and acetylcholinesterase (AChE) activity in mice in a dose-dependent manner. Additionally, parapyruvate induced Tau and phosphorylated Tau (p-Tau) aggregation at dosages ≥5 mg/kg/day and increased the myelin basic protein (MBP) expression at a dosage of 500 mg/kg/day. These results suggest that the equivalent dose of parapyruvate can induce neurodegeneration in the C57BL/6JNarl mice.

show abstract

“…The Taguchi method has been widely used in various industries, including manufacturing, engineering, and product development, to improve quality, reduce variability, and enhance overall performance. It provides a systematic and efficient approach to experimental design and optimization, making it a valuable tool for quality improvement initiatives [18][19][20][21][22]. For example, the Taguchi experimental design was applied to optimize the vacuum printing encapsulation process, where a combination of process parameters was considered, including the squeegee moving speed, printing delay time, and the number of squeegee printings [18].…”

Section: Introductionmentioning

confidence: 99%

Improvement in Laptop Heat Dissipation with Taguchi Method

Chang,

Chu,

Lin

2024

Electronics

View full text Add to dashboard Cite

This paper aims to investigate the feasibility of using system power consumption as a factor to improve laptop heat dissipation. The problems due to the CPU overheating are addressed. Based on the Taguchi method, the laptop fan parameters can be optimized with firmware adjustments only. In the Taguchi analysis, the fan speed, system power, and debounce time are considered as control factors, while the Cinebench point is utilized to evaluate the CPU performance. Experimental results demonstrate that the proposed heat dissipation scheme effectively reduces the idle time of a laptop fan. The improvement in heat dissipation can reduce CPU performance degradation because of overheating. According to the best combination of control factors, there is approximately a 5% increase in CPU performance despite a 0.35% increment in power consumption. This paper highlights the effectiveness of optimizing laptop fan parameters through firmware adjustments to improve heat dissipation and mitigate CPU overheating issues. Moreover, the study highlights the delicate balance between power consumption and performance gains. While there may be a slight increase in power consumption associated with the optimized heat dissipation scheme, the observed improvements in CPU performance outweigh this incremental power usage.

show abstract

Using Taguchi Method to Determine the Optimum Conditions for Synthesizing Parapyruvate

Cited by 6 publications

References 26 publications

Ambient temperature CO2 fixation to pyruvate and subsequently to citramalate over iron and nickel nanoparticles

Ambient temperature CO2 fixation to pyruvate and subsequently to citramalate over iron and nickel nanoparticles

Parapyruvate Induces Neurodegeneration in C57BL/6JNarl Mice via Inhibition of the α-Ketoglutarate Dehydrogenase Complex

Improvement in Laptop Heat Dissipation with Taguchi Method

Contact Info

Product

Resources

About