Background: In the United States, sudden infant death syndrome (SIDS) is the leading cause of death in infants aged 1 month to 1 year. Approximately 3,500 infants die from SIDS and sleeprelated reasons on a yearly basis. Unintentional sleep-related deaths and bed sharing, a known risk factor for SIDS, are on the rise. Furthermore, ethnic disparities exist among those most affected by SIDS. Despite public health campaigns, infant mortality persists. Given the popularity of social media, understanding social media conversations around SIDS and safe sleep may provide the medical and public health communities with the information needed to spread, reinforce, or counteract false information regarding SIDS and safe sleep.Objective: The purpose of this project was to study the social media conversation around SIDS to understand possible influences and guide health promotion efforts, public health research, and enable health professionals to engage in directed communication regarding this topic.Methods: This study used textual analytics to identify topics and extract meanings contained in unstructured textual data. Twitter messages were captured during the months of September, October, and November of 2017. Tweets and retweets were collected using NUVI software in conjunction with Twitter's Search API using the keywords: "sids", "infant death syndrome", "sudden infant death syndrome", and "safe sleep." This returned a total of 41,358 messages, which were analyzed using text-mining and social media monitoring software.Results: Multiple themes were identified, including: recommendations for safe sleep to prevent SIDS, safe sleep devices, the potential causes of SIDS, and how breastfeeding reduces SIDS. Compared to the months of September and November, October (Pregnancy and Infant Loss Awareness Month) demonstrated personal and specific stories of infant loss. The top influencers were news organizations, universities, and health-related organizations.Conclusions: This study offers valuable information regarding the public's perception and opinions regarding SIDS and safe sleep. It highlights the contradicting information the public is exposed to regarding SIDS and the continued controversy of vaccines. This analysis also emphasizes the lack of public health organizations' presence on Twitter compared to the influence of universities and news media organizations. It also demonstrates the prevalence of safe sleep products that are embedded in safe sleep messaging. These findings can assist providers in speaking about relevant topics when engaging in conversation about the prevention of SIDS and the promotion of safe sleep. Further, public health agencies and advocates should utilize social media and Twitter to better communicate accurate health information as well as continue to combat the spread of false information.