Using the liquid nature of the stationary phase in countercurrent chromatography

Berthod, Alain; Hassoun, Mahmoud

doi:10.1016/j.chroma.2006.03.031

Cited by 39 publications

(16 citation statements)

References 16 publications

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“…High‐speed countercurrent chromatography (HSCCC) 12 was performed in a Pharma‐Tech chromatograph model CCC‐1000. The three coils (360 ml) were first filled with the lower organic phase of a mixture of dichloromethane‐methanol‐water (5 : 6 : 4).…”

Section: Methodsmentioning

confidence: 99%

Antifungal activity of trichothecenes from Fusarium sp. against clinical isolates of Paracoccidioides brasiliensis

Campos¹,

Johann²,

Cota³

et al. 2010

Mycoses

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Paracoccidioidomycosis (PCM), a human mycosis caused by the dimorphic fungus Paracoccidioides brasiliensis, is a serious public health problem in several countries of Latin America. In our search we found that the crude extract of the endophytic fungus UFMGCB 551 was able to inhibit several clinical strains of P. brasiliensis, and was also active in the bioautographic assay against Cladosporium sphaerospermum. The endophytic fungus UFMGCB 551 was isolated from the plant Piptadenia adiantoides J.F. Macbr (Fabaceae). The fungus was identified as Fusarium sp. based on its macro- and micro-morphology, and on the sequence of the internally transcribed spacer regions (ITS) of its rRNA gene. The chromatographic fractionation of the fungal extract was guided by the bioautographic assay to afford three known trichothecene mycotoxins: T2-toxin (1) and a mixture of 8-n-butyrylneosolaniol (2) and 8-isobutyrylsolaniol (3). The minimal inhibitory concentrations (MIC) of the these compounds against eleven clinical strains of P. brasiliensis were evaluated and found to be in the range between 75 and 640 nmol l(-1) for 1 and 160-640 nmol l(-1) for the mixture of 2 and 3.

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Section: Methodsmentioning

confidence: 99%

Antifungal activity of trichothecenes from Fusarium sp. against clinical isolates of Paracoccidioides brasiliensis

Campos¹,

Johann²,

Cota³

et al. 2010

Mycoses

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“…This technique is frequently adopted at the end of standard CCC purification runs simply to ensure that no loss of target compound has occurred within the liquid stationary phase. Another possibility is co-current chromatography (Berthod and Hassoun 2006) where both phases are pumped in the same direction. All of these options use a conventional CCC centrifuge and are thus easy to implement in the laboratory or pilot plant.…”

Section: Principle Advantages and Drawbacks Of CCC In The Purificatiomentioning

confidence: 99%

Counter-current chromatography for the separation of terpenoids: a comprehensive review with respect to the solvent systems employed

Skalicka-Woźniak

Garrard

2014

Phytochem Rev

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Natural products extracts are commonly highly complex mixtures of active compounds and consequently their purification becomes a particularly challenging task. The development of a purification protocol to extract a single active component from the many hundreds that are often present in the mixture is something that can take months or even years to achieve, thus it is important for the natural product chemist to have, at their disposal, a broad range of diverse purification techniques. Counter-current chromatography (CCC) is one such separation technique utilising two immiscible phases, one as the stationary phase (retained in a spinning coil by centrifugal forces) and the second as the mobile phase. The method benefits from a number of advantages when compared with the more traditional liquid–solid separation methods, such as no irreversible adsorption, total recovery of the injected sample, minimal tailing of peaks, low risk of sample denaturation, the ability to accept particulates, and a low solvent consumption. The selection of an appropriate two-phase solvent system is critical to the running of CCC since this is both the mobile and the stationary phase of the system. However, this is also by far the most time consuming aspect of the technique and the one that most inhibits its general take-up. In recent years, numerous natural product purifications have been published using CCC from almost every country across the globe. Many of these papers are devoted to terpenoids—one of the most diverse groups. Naturally occurring terpenoids provide opportunities to discover new drugs but many of them are available at very low levels in nature and a huge number of them still remain unexplored. The collective knowledge on performing successful CCC separations of terpenoids has been gathered and reviewed by the authors, in order to create a comprehensive document that will be of great assistance in performing future purifications.

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“…Another mode where the phases both move in the same direction, is referred to as co-current CCC [26]. Recently, modelling on this type of CCC has been proposed by Berthod and Hassoun [27]. …”

Section: What Are the Applications Of CCC Models?mentioning

confidence: 99%

Recent Progress on Countercurrent Chromatography Modeling

Wang

Ito

Wei

2014

Journal of Liquid Chromatography & Related Technologies

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As countercurrent chromatography is becoming an established method in chromatography for many kinds of products, it is becoming increasingly important to model the process and to be able to predict the peaks for a given process. The CCC industries are looking for rapid methods to analyze the processes of countercurrent chromatography and select suitable solvent system. In this paper, recent progress is reviewed in the development and demonstration of several types of models of countercurrent chromatography. Literature lists a number of countercurrent chromatography (CCC) models that can predict the retention time and to a certain extent the peak width of a solute eluting from a CCC column, such as cell model, CCD model, CSTRs model, probabilistic model, temperature dependence plate model, physical models, etc.

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Using the liquid nature of the stationary phase in countercurrent chromatography

Cited by 39 publications

References 16 publications

Antifungal activity of trichothecenes from Fusarium sp. against clinical isolates of Paracoccidioides brasiliensis

Antifungal activity of trichothecenes from Fusarium sp. against clinical isolates of Paracoccidioides brasiliensis

Counter-current chromatography for the separation of terpenoids: a comprehensive review with respect to the solvent systems employed

Recent Progress on Countercurrent Chromatography Modeling

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