Using UV-C radiation and image analysis for fungus control in tomato plants

Valencia, Muñoz; Patiño, Leydi Fernanda Vásquez; Herrera-Ramírez, Jorge; Castañeda, Doris; Gómez, J. A.; Quijano, José Carlos Arévalo

doi:10.7149/opa.50.4.49073

Cited by 4 publications

(4 citation statements)

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“…The results demonstrate that a UV-C dose of 1,080 J ⋅ m -2 significantly reduced gray mold in the petal, after 24 h of inoculation with the fungus. Other studies showed similar results concerning reduction of gray mold disease in other species with UV-C treatments (Janisiewicz et al 2016;Valencia et al 2017). However, we found that after 27 h of inoculation, the treatment was not as effective as 24 h after inoculation.…”

Section: Discussionsupporting

confidence: 78%

“…To be able to penetrate the surface of the host, a pathogenic fungi uses an infection structure (appressorium) that forms a penetration pin that breaks the cuticle (van Kan 2006). When the conidia of the fungi are irradiated with UV light, they lose the capacity to develop these structures, as also evidenced by Janisiewicz et al (2016), Valencia et al (2017 and Zhu et al (2019). Additionally, the formation of dimers in DNA, caused by UV-C, impairs the action of DNA polymerase during elongation of germ tubes.…”

Section: Discussionmentioning

confidence: 99%

“…This light is used on several surfaces, as well as in air and water, due to its germicidal effects (Levetin et al 2001;Yin et al 2013). UV light is a viable alternative method to control postharvest diseases of fruits and vegetables, by acting directly on pathogens or indirectly through the induction of defenses both in plants as well as in harvested products (Ben-Yehoshua et al 1992;Stevens et al 1996Stevens et al , 1998de Capdeville et al 2002;Romanazzi et al 2012;Gutiérrez et al 2016;Mohamed et al 2017;Valencia et al 2017). This light has shown promising performance to control decomposition and to extend shelf life of postharvested plant products (Nigro et al 1998;Vicente et al 2005;Nigroa and Ippolito 2016;Jin et al 2017;Karasahin Yildirim and Pekmezci 2017;Ortiz Araque et al 2018).…”

Section: Uv-c Treatment On Flowersmentioning

confidence: 99%

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Journal of Plant Protection Research

Vega,

Ochoa,

Patiño

et al. 2020

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The shipment of cut flowers from Colombia and Ecuador to the United States, the biggest importer of this product in the world, has doubled in the last 20 years. One of the main constraints in cut roses production is the gray mold disease caused by the fungus Bot rytis cinerea, which can destroy the flowers, in the crop, during storage and/or shipping. Since the resistance of the fungus to conventional fungicides has been increasing, as well as the health effects in rose growers, alternative approaches for controlling the disease are needed. The effect of UV-C light on the gray mold development in cut roses was studied. Irradiation with 2,160; 1,080 and 540 J ⋅ m -2 UV-C, every 24 h for 5 days in a humid chamber, did not harm the roses. Instead, as seen by image analysis, a highly significant reduction of the area of the lesions by the disease and of the fungus germination was obtained at 1,080 J ⋅ m -2 . The addition of a 4-h dark period to the irradiation did not improve the effect of UV-C on the disease. The results of this work potentiate the use of UV-C light in the agro-industry as a low-cost and non-invasive alternative method to control diseases. They also reflect the application of optical approaches as image analysis in the evaluation of important agricultural features.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Uv-c Treatment On Flowersmentioning

confidence: 99%

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Journal of Plant Protection Research

Vega,

Ochoa,

Patiño

et al. 2020

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“…Numerous studies have shown the beneficial effects of postharvest UV-C hormesis in several plant species (for a review, see Duarte-Sierra et al, 2019 ). When applied at the preharvest stage, the beneficial effects of UV-C hormesis have been shown in strawberry ( Janisiewicz et al, 2016a , b ; Xie et al, 2016 ; Xu et al, 2019 ), tomato ( Valencia et al, 2017 ), and lettuce ( Vàsquez et al, 2017 ; Sidibé et al, 2021 ). Among the beneficial effects of UV-C on lettuce, Sidibé et al (2021) showed an increase in dry matter and resistance against Xcv with no negative impact on yield.…”

Section: Introductionmentioning

confidence: 99%

Preharvest UV-C Hormesis Induces Key Genes Associated With Homeostasis, Growth and Defense in Lettuce Inoculated With Xanthomonas campestris pv. vitians

et al. 2022

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Preharvest application of hormetic doses of ultraviolet-C (UV-C) generates beneficial effects in plants. In this study, within 1 week, four UV-C treatments of 0.4 kJ/m2 were applied to 3-week-old lettuce seedlings. The leaves were inoculated with a virulent strain of Xanthomonas campestris pv. vitians (Xcv) 48 h after the last UV-C application. The extent of the disease was tracked over time and a transcriptomic analysis was performed on lettuce leaf samples. Samples of lettuce leaves, from both control and treated groups, were taken at two different times corresponding to T2, 48 h after the last UV-C treatment and T3, 24 h after inoculation (i.e., 72 h after the last UV-C treatment). A significant decrease in disease severity between the UV-C treated lettuce and the control was observed on days 4, 8, and 14 after pathogen inoculation. Data from the transcriptomic study revealed, that in response to the effect of UV-C alone and/or UV-C + Xcv, a total of 3828 genes were differentially regulated with fold change (|log2-FC|) > 1.5 and false discovery rate (FDR) < 0.05. Among these, of the 2270 genes of known function 1556 were upregulated and 714 were downregulated. A total of 10 candidate genes were verified by qPCR and were generally consistent with the transcriptomic results. The differentially expressed genes observed in lettuce under the conditions of the present study were associated with 14 different biological processes in the plant. These genes are involved in a series of metabolic pathways associated with the ability of lettuce treated with hormetic doses of UV-C to resume normal growth and to defend themselves against potential stressors. The results indicate that the hormetic dose of UV-C applied preharvest on lettuce in this study, can be considered as an eustress that does not interfere with the ability of the treated plants to carry on a set of key physiological processes namely: homeostasis, growth and defense.

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