Using virulence genes hrpB, egl and fliC in differentiation between virulent and avirulent isolates of Ralstonia solanacearum, the causal agent of potato brown rot

Mikhail, M. S.

doi:10.5897/ajmr11.295

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Spunta) grown under greenhouse conditions appeared in form of yellowing or sudden leaf wilting with down bending of these leaves before appearance the whole death onto potato plants. The recorded symptoms of brown rot disease are similar to those recorded by Mikhail et al (2012) stated that R. solanacearum (Six virulent and three avirulent) recovered from the natural habitats (potato tubers, weeds, soil and water) were pathogenic to potato plants . El-Haj Saleh (2014) found that when potato plants were injected with R. solanacearum isolates, wilt symptoms appeared compared to non-injected potato plants this result confirms that all the tested isolates are virulent and belong to race 3 biovar 2.…”

Section: Virulence Of R Solanacearum Isolates On Potato Plants Under Greenhouse Conditionsupporting

confidence: 82%

Molecular Characteristics of Ten Ralstonia solanacearum Strains of Brown Rot Disease in Potato from three Governorates in Egypt

Serag

Salim

Farid

et al. 2020

Journal of Agricultural Chemistry and Biotechnology

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Using tests based on serological, chemical and pathological aspects Ralstonia solanacearum strains (of brown rot disease) were identified in 10 strains were isolated from infected potato tubers grown in Egypt with biovar II pathogenicity and hypersensivity on leaves of tobacco and sugar utilization. , ISSR markers were used to differentiate the strains .Total number of amplified fragments was 68 bands and polymorphic bands were 38 representing 56% polymorphism. Twenty one bands were monomorphic and 9 were uniquely amplified DNA fragments of DNA which ranged between 7 to 16. The number of polymorphic fragments ranged between 1 to 13 for each primer. Values of genetic similarity among genotypes ranged from 63 to 92%. A dendrogram separated the strains into two major clusters. Pathogenicity tests showed QB.4, BK.5, BK.7 and Gk.8 isolates being highly virulent on tobacco in hypersensitivity test (HR) study. Virulence degree were between low and high on seedlings of tomato and potato and tomato seedlings. R. solanacearum (QB.4, QB.5, BK.7 and BK.8) showed highest disease incidence of 25.0, 24.6, 25.3 and 28.5%, respectively. Recorded wilt disease severity were 6.5, 6.2, 6.1 and 7.5 %, respectively at 42 days. Only the four isolates QB.4, QB.5, BK.7 and BK.8 were highly pathogenic to all of the studied seven potato cultivars of Accent,

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Section: Virulence Of R Solanacearum Isolates On Potato Plants Under Greenhouse Conditionsupporting

confidence: 82%

Molecular Characteristics of Ten Ralstonia solanacearum Strains of Brown Rot Disease in Potato from three Governorates in Egypt

Serag

Salim

Farid

et al. 2020

Journal of Agricultural Chemistry and Biotechnology

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“…Ralstonia solanacearum expresses the hrp gene encoded proteins which are pathogenic to tomato plants and elicits a hypersensitive response (HR) after infilteration of the leaves (Genin et al 1992;Boucher 2004). et al 1992;Kanda et al 2003;Mikhail et al 2012). According to Occhialini et al (2005), the hrpB gene encodes a transcriptional regulator araC (1-b-D-arabino furanosyl cytosine) type protein which governs the multiple virulence pathways.…”

Section: Introductionmentioning

confidence: 98%

Detection, Identification and Phylogenetic Analysis of Indian Ralstonia solanacearum Isolates by Comparison of Partial hrpB Gene Sequences

Chandrasekhar

Umesha

2014

Journal of Phytopathology

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A species‐specific Polymerase Chain Reaction (sPCR) method was developed to identify and detect isolates of Ralstonia solanacearum, the cause of bacterial wilt disease in chilli. PCR primers for R. solanacearum were identified by alignment of hrpB gene sequences and selection of sequences specific for R. solanacearum at their 3′ ends. The primers were shown to be specific for R. solanacearum, as no PCR product was obtained when genomic DNA from other bacterial species including closely related Ralstonia species, were used as test species. Lone pair of primers (RshrpBF and RshrpBR) was designed using hrpB gene sequence, unique to R. solanacearum which amplified a predicted PCR product of 810 bp from 20 different isolates. Phylogenetic analysis was also attempted to understand the evolutionary divergence of Indian R. solanacearum isolates. Based on phylogenetic analysis, Indian isolates showed homology with the standard reference isolates from other countries but, interestingly, one new isolate showed complete evolutionary divergence by forming an out‐group.

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Investigation of virulence diversity in Ralstonia solanacearum isolates by a random amplified polymorphic DNA collected from Egyptian potato fields

Bereika

Moharam

Abo-Elyousr

et al. 2022

Archives of Phytopathology and Plant Protection

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Using virulence genes hrpB, egl and fliC in differentiation between virulent and avirulent isolates of Ralstonia solanacearum, the causal agent of potato brown rot

Cited by 3 publications

References 20 publications

Molecular Characteristics of Ten Ralstonia solanacearum Strains of Brown Rot Disease in Potato from three Governorates in Egypt

Molecular Characteristics of Ten Ralstonia solanacearum Strains of Brown Rot Disease in Potato from three Governorates in Egypt

Detection, Identification and Phylogenetic Analysis of Indian Ralstonia solanacearum Isolates by Comparison of Partial hrpB Gene Sequences

Investigation of virulence diversity in Ralstonia solanacearum isolates by a random amplified polymorphic DNA collected from Egyptian potato fields

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