2014
DOI: 10.2306/scienceasia1513-1874.2014.40.157
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Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii

Abstract: Vitellogenesis in crustaceans is under the inhibitory regulation of the endocrine system in the X-organ/sinus gland complex in the eyestalks. In this study, two monoclonal antibodies specific to Macrobrachium rosenbergii vitellin and vitellogenin were generated to assess vitellogenesis-inhibiting hormone (VIH) activity. A combination of two monoclonal antibodies specific to the 89 and 100 kDa subunits of vitellin were used to develop a competitive enzyme-linked immunosorbent assay protocol for the quantitative… Show more

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