We want to acknowledge the Michigan State University W.J. Beal Botanical Gardens and Campus Arboretum (Frank W. Telewski, and Jeffrey Wilson) and the Michigan State University Department of Infrastructure, Planning and Facilities (Jerry Wahl) who assisted with the urban data collection. Furthermore, we want to thank the research staff of Harvard Forest (David Orwig, Audrey Barker Plotkin), Alan Strahler and UNAVCO for coordinating the rural forest data collection. We would also like to thank Samuel Clark, Garret Dettmann and the field group of Michigan State University, Jereme Frank (University of Maine), David Walker and Phil Radtke (Virginia Tech University) for their contribution to the collection of destructive tree measurements in Harvard Forest. Finally, we want to acknowledge the contribution of Matheus 2 B. Vicari (University College London) for processing the point clouds of the evergreen species scanned by K. Calders in Harvard Forest.Key Message: TLS data can be converted to reliable woody AGB estimates, but estimation quality is influenced by growing environment, leaf condition, and variation in tree density affecting volume to mass conversion.