To better understand the dynamics of online student test taking, including the likelihood of cheating by large numbers of students, we examined test-taking patterns and outcomes of weekly online quizzes in two large undergraduate biology lecture courses. Students taking a quiz late in a 1-3-day quiz access period performed 10-15% worse on quizzes than the students who completed the quiz early. Quiz access time was also negatively correlated with performance in other course components and course grades. These patterns suggest that academic dishonesty was not a determinant in unsupervised online quiz performance. Students generally completed quizzes in late afternoon or evening hours, but students who completed quizzes between midnight and 8 a.m. had significantly lower quiz grades than their peers. In addition, upper-division students were more likely to characterize weekly online quizzes as more helpful for their learning than the lower-division students.Keywords: Biology, cheating, web-based instruction, online examination.The use of online course management systems (CMS) 1 such as Blackboard, WebCT, and Moodle is becoming more common in higher education. In spring 2007, Montana State University (MSU), Bozeman, offered 299 courses on campus with a CMS component and 59 distance education courses delivered via CMS. Over 8,800 of MSU's 12,000 students (73%) had at least one course that used Web-CT in the school year [2006][2007]. In this article, we examine student performance on weekly, graded, online quizzes in two large biology lecture courses. We hypothesized that the majority of students would access the quiz late in the access period, and also hypothesized that average scores would increase over the access period as early quiz-takers shared information about quiz content with fellow students.Web-based activities, including online quizzes, can increase student performance in a course, suggesting that these activities are worthwhile. DeSouza and Fleming show that students who practice for exams using online quizzes outperform peers who take traditional paper and pencil quizzes [1]. Freeman et al. [2] indicate that online graded practice exams, as part of a strategy of active learning exercises, increase the performance of students in introductory biology, especially those at high risk for failing the course. Riffell and Sibley [3] also show that hybrid course formats that use online assignments to promote active learning improve student performance. Web-based activities also provide greater flexibility for students to choose when they will complete activities, and may increase student learning by increasing the participation in course activities [4,5].One great concern in using online assignments, particularly online testing, is the potential for academic dishonesty. Even in the absence of computers, it is clear that cheating is prevalent in our educational system [6]. At least 70% of high-school students admit to having cheated [7]. More recent studies commissioned by the Educational Testing Service (ETS) ind...