Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Fatibello‐Filho, Orlando; Vieira, Iolanda Cruz

doi:10.1590/s0100-40422002000300019

Cited by 84 publications

(57 citation statements)

References 101 publications

(161 reference statements)

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“…Os extratos das amostras foram preparados pela homogeneização de 0,1 g de matéria fresca em 4 mL do tampão fosfato de sódio 0,1 M (pH 6.5) adicionados de 0,05 g de polivinilpirrolidona (PVP). O homogenato foi centrifugado a 10.000 x g a 4 ºC, por 10 min (Zeraik et al, 2008) e realizada a análise das proteínas solúveis totais (Bradford, 1976) e das seguintes enzimas:, catálase (Berrs & Sizer, 1952), ascarbato peroxidase (Nakano & Asada, 1981), polifenoloxidase (Kar & Mishra, 1976) e peroxidase (Fatibelho Filho & Vieira, 2002). Já para a análise do aminoácido prolina, foi preparado o extrato obtido de 0,1 g de matéria fresca em 5 mL de ácido sulfosalicílico a 3%.…”

Section: Methodsunclassified

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Sousa¹,

Pedrosa

Rolim

et al. 2015

Rev. bras. eng. agríc. ambient.

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R E S U M OConsiderando a importância do feijoeiro caupi no Nordeste brasileiro, o presente estudo teve, por objetivo, avaliar o crescimento e os aspectos bioquímicos do cultivar EPACE-10 submetido a estresse hídrico e parasitismo de Meloidogyne incognita em condições de casa de vegetação. O delineamento adotado foi de blocos ao acaso em esquema fatorial 2 (com e sem nematoide) × 2 (com e sem estresse hídrico), com seis repetições. O nível de estresse hídrico aplicado (50% da capacidade do campo) diminuiu a reprodução do nematoide; entretanto, o desenvolvimento das plantas aferido pelas biomassas frescas e secas do caule e folhas, número de folhas, índice de área foliar, altura da planta e pela biomassa fresca da raiz, não foi afetado pelo estresse hídrico aplicado. As respostas das enzimas oxidativas ao estresse hídrico e parasitismo de M. incognita foram variáveis. A peroxidase não foi afetada, a catálase foi diminuída pelo parasitismo do nematoide, a ascorbato peroxidade diminuída pelo estresse hídrico e a polifenoloxidase diminuída pelo estresse hídrico e pelo parasitismo de M. incognita. Growth and enzymatic responses of cowpea under water stress and root-knot nematode A B S T R A C TConsidering the importance of cowpea beans in the Northeast of Brazil, the present study aimed to evaluate growing and biochemical aspects of cultivar EPACE-10 subjected to water stress and parasitism of Meloidogyne incognita under greenhouse conditions. The design was a randomized block design in a factorial 2 (with and without nematode) × 2 (with and without water stress), with six replications. The level of applied water stress (50% of the field capacity) reduced the nematode reproduction. However, plant growth measured by fresh and dry biomass of stems and leaves, leaf number, leaf area index, plant height and fresh root biomass was not affected by water stress applied. The responses to water stress and oxidative enzymes and parasitism of M. incognita were variable. Peroxidase was not affected; catalase was decreased by nematode parasitism, ascorbate peroxidase decreased by water stress and polyphenoloxidase decreased by water stress and parasitism of M. incognita. Palavras-chave:Vigna unguiculata Meloidogyne incognita enzimas oxidativas

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Section: Methodsunclassified

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Sousa¹,

Pedrosa

Rolim

et al. 2015

Rev. bras. eng. agríc. ambient.

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“…Mattos et al 109 have also developed glucose biosensors using the enzyme glucose oxidase immobilized on Prussian blue films electrodeposited on glassy carbon electrodes. The analytical use of vegetable tissue or crude extracts as enzymatic sources has been extensively studied by Fatibello and co-workers [121][122][123][124][125] and by Angnes and coworkers. 126 Examples of many biosensors and/or flow injection procedures for analysis of compounds of biological, environmental, food, pharmaceutical and industrial interest habe been described using vegetable tissues or crude extracts from sweet potato, 122 avocado, 123 zucchini, peach, yam, cassava, artichoke, turnip, horseradish, 124,125 and tissues from fruits of the palm tree latania sp.…”

Section: Amperometric Biosensorsmentioning

confidence: 99%

Electrochemical sensors: a powerful tool in analytical chemistry

Stradiotto¹,

Yamanaka²,

Zanoni³

2003

J. Braz. Chem. Soc.

334

154

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Sensores potenciométricos, amperométricos e condutométricos são sensores eletroquímicos com imensa aplicação nas áreas de meio ambiente, indústria e análises clínicas. O presente trabalho apresenta uma revisão generalizada dos aspectos fundamentais, os desenvolvimento e a contribuição destes sensores na área de química analítica, e reporta alguns aspectos relevantes dos avanços na área de sensores eletroquímicos no Brasil.Potentiometric, amperometric and conductometric electrochemical sensors have found a number of interesting applications in the areas of environmental, industrial, and clinical analyses. This review presents a general overview of the three main types of electrochemical sensors, describing fundamental aspects, developments and their contribution to the area of analytical chemistry, relating relevant aspects of the development of electrochemical sensors in Brazil.

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“…Furthermore, the extract maintains the enzymatic activity for longer time. 13 One of the most critical steps in the design and assembly of biosensors is enzyme immobilization. If the recognizing element is not effectively attached, it will be drained to solution during analysis, thus reducing signal and sensibility of the constructed device.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Different Phenolic Compounds Using Electrochemical Biosensor and Multivariate Calibration

Dantas

Nogueira

Etchegaray

et al. 2017

J. Braz. Chem. Soc.

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Phenolic compounds are important environmental contaminants due to their high toxicity and persistence in the environment. The use of enzyme-based electrochemical biosensors is a simple, sensitive and low-cost alternative for the determination of these pollutants in contaminated waters. However, in most cases, it is impossible to detect specific compounds in a mixture of phenols due to signal-overlap, as the instruments operate at very close potentials, given that the system is based on a single enzyme to detect similar structures. In order to overcome this problem, in the present work we have successfully used multivariate calibration with partial least squares (PLS) for the simultaneous determination of hydroquinone and guaiacol by a tyrosinase-based biosensor that was assembled using an enzyme extract from yam. The use of PLS allowed us to work with a large number of voltammograms, leading to a single mathematical model for the simultaneous determination of phenols of similar structure in real samples with concentration values of mmol L -1 .

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Uso analítico de tecidos e de extratos brutos vegetais como fonte enzimática

Cited by 84 publications

References 101 publications

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Electrochemical sensors: a powerful tool in analytical chemistry

Simultaneous Determination of Different Phenolic Compounds Using Electrochemical Biosensor and Multivariate Calibration

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