Uso De Culturas Probióticas Em Produtos Cárneos Fermentados

Batista, Nayane Valente; Gadelha, Ana Indira Bezerra Barros; Batista, Fernanda Keila Valente; Souza, Isis Thamara do Nascimento; Oliveira, Jéssica Taiomara Moura Costa Bezerra de; Pinto, Márcia Marcila Fernandes; Oliveira, Palloma Vitória Carlos de; Batista, Scarlett Valente; Melo, Vitor Lucas de Lima

doi:10.22533/at.ed.98019091017

Cited by 1 publication

(2 citation statements)

References 28 publications

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“…Qualitative screening of fungal isolates for amylase producers All the pure isolates obtained were screened for actual and efficient starch degraders. The method described by (Batista et al (2018) was adopted with slight modification using starch hydrolysis approach on PDA. The pure fungal cultures were inoculated on a freshly prepared starch agar medium and plates were incubated for 48 h at room temperature (37 o C).…”

Section: Purification and Identification Of Fungal Isolatesmentioning

confidence: 99%

“…After 48 h of incubation, the plates were flooded with the Lugol's iodine to determine any starch hydrolysis. Clearing zones around the colonies were measured and used to evaluate the level of amylase activity (Batista et al, 2018). This is as indication of starch hydrolysis by the fungi isolates which reflects amylase secretion.…”

Section: Purification and Identification Of Fungal Isolatesmentioning

confidence: 99%

See 1 more Smart Citation

Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Ire

Wokoma

Okoli

2020

JLBSR

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Amylases (E.C.3.2.1.1) are enzymes which catalyze the breakdown of -1,4 glycosidic linkages of starch to simple sugars and different monomeric products. Microbial amylases are essential enzymes compared with animals and plants amylases in industrial applications. Thus, in southern Nigeria, garri processing waste-water from mills poses a serious environmental challenge and this situation could be exploited by utilizing microorganisms colonizing the area to manufacture microbial products. This present study was aimed at evaluating the potential of fungi isolated from garri processing environment for amylase secretion and conduct a time course study of the enzyme production. Fungi were isolated from the soil and waste water from garri processing sites using Standard Microbiological Procedures. The five fungi isolated from garri processing environmental samples were screened using Lugols iodine. Three fungal isolates were then selected on the hydrolysis of starch in qualitative zonation agar plates but one isolate named B among them was better in starch hydrolysis related to highest clear zone plates. After qualitative screening, the three hyper production amylase extracted fungi were identified according to the morphological characteristics. The hyper producing amylase isolate B was then identified as Aspergillus flavus (RCBBR_AEAFUN2) and recorded as a novel strain in southern Nigeria according to molecular characteristics, which was selected for further studies. The current results of time course study showed that the maximum growth (2.453 g) occurred after 24 h of incubation at pH 4.5 while maximum enzyme production (2.3 U/ml/min) was obtained after 96 h of incubation at pH 3.5. Therefore, the hyper producing amylase from garri processing sites, Aspergillus flavus (RCBBR_AEAFUN2) novel strain has great prospects for future biotechnological and industrial applications and help in ameliorating the environmental pollution posed by the waste-water.

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Section: Purification and Identification Of Fungal Isolatesmentioning

confidence: 99%

Section: Purification and Identification Of Fungal Isolatesmentioning

confidence: 99%