Uso de nanopartícula lipídica como veículo do quimioterápico docetaxel no tratamento da aterosclerose induzida em coelhos

Abstract

Meneghini C.B. Use of lipid core nanoparticle as a vehicle of the chemotherapeutic docetaxel in the treatment of atherosclerosis induced in rabbits [thesis]. São Paulo: "Faculdade de Medicina, Universidade de São Paulo"; 2018. Considered as a new strategy of targeting drugs to injured tissues, LDE, a lipid core nanoparticle concentrates on inflammatory sites with high cell proliferation rates, as atherosclerotic lesions, and it is used as a vehicle for drugs in experimental models and in humans. Docetaxel (DTX), an antiproliferative chemotherapeutic agent has not been explored yet in the treatment of atherosclerosis. New Zealand white male rabbits were fed with 1% cholesterol diet throughout the 8-week experimental period to induce atherosclerosis. After 4 weeks, the animals were treated weekly with LDE-DTX (n=9) at a dose of 1mg/kg i.p. or only with LDE (Control group, n=9). We evaluated feed intake, lipid, hematological, and weight profiles during the protocol at baseline, 4 weeks and post-treatment. After euthanasia was performed morphological analysis and Western blot of the aorta. As expected, total cholesterol increased 42-fold in both groups comparing baseline to post-treatment. There was a decrease in red blood cells number in both groups but it is probably not treatment-related. There was no hepatic and renal treatment-related toxicity.