Uterine infusion of bacteria alters the transcriptome of bovine oocytes

Piersanti, Rachel L.; Block, J.; Ma, Zhengxin; Jeong, Kwangcheol C.; Santos, J. E. P.; Yu, Fahong; Sheldon, I. Martin; Bromfield, John J.

doi:10.1096/fba.2020-00029

Cited by 9 publications

(7 citation statements)

References 46 publications

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“…Additionally, invasion by pathogenic microbes and their toxins induce inflammation, triggering host inflammatory responses and destruction of the endometrial integrity in cattle reproductive organs, which are unfavourable conditions for the transport of spermatozoa and embryonic development [ 34 – 36 ]. Infestation of pathogens on the bovine gametes detriments the reproductive efficiency by interfering the development of oocytes [ 37 , 38 ] and reducing the sperm functionality including sperm viability, motility and DNA integrity [ 39 – 42 ]. Overgrowth of opportunistic pathogens is also not beneficial during early embryonic development as it increases the risk of early embryonic death, abortion or birth of an abnormal or persistently infected calf [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

et al. 2021

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Undesirable microbial infiltration into the female bovine reproductive tracts, for example during calving or mating, is likely to disturb the commensal microflora. Persistent establishment and overgrowth of certain pathogens induce reproductive diseases, render the female bovine reproductive tract unfavourable for pregnancy or can result in transmission to the foetus, leading to death and abortion or birth abnormalities. This review of culture-independent metagenomics studies revealed that normal microflora in the female bovine reproductive tract is reasonably consistently dominated by bacteria from the phyla Bacteroidetes, Firmicutes, Proteobacteria, following by Actinobacteria, Fusobacteria and Tenericutes. Reproductive disease development in the female bovine reproductive tract was demonstrated across multiple studies to be associated with high relative abundances of bacteria from the phyla Bacteroidetes and Fusobacteria. Reduced bacterial diversity in the reproductive tract microbiome in some studies of cows diagnosed with reproductive diseases also indicated an association between dysbiosis and bovine reproductive health. Nonetheless, the bovine genital tract microbiome remains underexplored, and this is especially true for the male genital tract. Future research should focus on the functional aspects of the bovine reproductive tract microbiomes, for example their contributions to cattle fertility and susceptibility towards reproductive diseases.

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Section: Introductionmentioning

confidence: 99%

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

et al. 2021

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“…This response could be explained by the potentially greater endometrial injury or incomplete uterine recovery in heifers undergoing clinical metritis. Also, uterine infections may reduce oocyte quality (Piersanti et al 2020) and its capacity to develop to morulae (Dickson et al 2020), which could have a carryover effect that could interfere with the completion of fetal development. An additional possible scenario is that the impact of puerperal metritis on the immune system persists weeks after the uterine in ammation has been resolved (Magata et al 2016); therefore, there are long-term alterations of systemic immune responses which possibly interfere with the maintenance of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Risk factors associated with testing positive for brucellosis and occurrence of abortion in high-yielding Holstein heifers

Luna

Encina

Álvarez

et al. 2022

Preprint

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This retrospective study aimed to estimate the seroprevalence of bovine brucellosis (bBR) in high-yielding Holstein heifers and identify risk factors associated with seropositivity to this disease. An additional aim was to assess the heifer-related risk factors for abortion. This study was carried out on a commercial dairy farm in northern Mexico (25° N) using 3848 heifers from 2018 to 2021. The seroprevalence of bBR was 30% (95% CI = 29–32%) for lactating heifers (card test). Multiple logistic regression models indicated that heifers with > 680 kg at calving had half the risk of being positive to bBR than heifers with < 680 kg at calving (22.7 vs. 36.1%). Heifers suffering from diarrhea during the growing period were 1.3 times more likely to be seropositive to bBR than heifers not diagnosed with this disease (34.1 vs. 29.4%; P < 0.01). The risk of being seropositive to bBR increased in heifers suffering from retained placenta compared with heifers not suffering from this reproductive disorder (OR = 2.46; 47.5 vs. 26.3%). Heifers vaccinated with two doses of commercial vaccines against brucellosis were associated (P < 0.01) with 2.2 times higher odds of being seropositive to bBR than heifers vaccinated only once. Heifers seropositive to bBR were 3.1 times more likely to abort than heifers seronegative to this disease (38.5 vs. 16.2%; P < 0.01). Heifers with body condition score (BCS) ≥ 3.5 at calving (OR = 0.3) had a protective role for seropositive to bBR. The likelihood of having an abortion was 3.1 times higher in heifers diagnosed with retained placenta than in those not having this reproductive disorder. It was concluded that brucellosis control programs should focus on age at first calving, retained placenta, and the number of brucellosis strain RB51 vaccinations applied to heifers. Increasing BCS was advantageous to achieve lower abortion rates.

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“…The impact of metritis on establishment and maintenance of pregnancy after first AI encompasses changes in the uterus, ovarian follicles, and enclosed oocytes. Uterine infusion of pathogenic Escherichia coli and Trueperella pyogenes altered the transcriptome of reproductive tissues (i.e., oocytes, granulosa cells, endometrium, and oviduct) and reduced the developmental capacity of oocytes fertilized in vitro (Dickson et al, 2020;Horlock et al, 2020;Piersanti et al, 2020). Moreover, exposure to LPS in vitro increased occurrence of meiotic arrest and failure of germinal vesicle breakdown (Bromfield and Sheldon, 2011).…”

Section: Discussionmentioning

confidence: 99%

Failure of clinical cure in dairy cows treated for metritis is associated with reduced productive and reproductive performance

Figueiredo

Merenda

Oliveira

et al. 2021

Journal of Dairy Science

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Objectives were to assess reproductive and productive outcomes associated with failure of clinical cure in dairy cows diagnosed with metritis following antimicrobial therapy. This retrospective cohort study included data from 3 experiments performed in 5 dairies. Metritis was characterized by the presence of watery, fetid, reddish-brownish vaginal discharge within 21 DIM (study d 0). Cows not diagnosed with metritis (i.e., cows may have had other diseases postpartum; NoMT; n = 1,194) were paired based on lactation number and calving date. All cows with metritis received antimicrobial therapy (ampicillin or ceftiofur). Clinical cure was evaluated on d 10 based on vaginal discharge score, and cows were categorized as cured (MTC; n = 1,111) or not cured (MTnoC; n = 299). Purulent vaginal discharge (28 ± 3 or 32 ± 3 DIM), cytological endometritis (35 ± 3 or 39 ± 3 DIM), and estrous cyclicity (50 ± 3 and 64 ± 3, 36 ± 3 and 50 ± 3, or 37 ± 5 and 51 ± 5 DIM) were evaluated in subgroups of cows. Proportions of cows with purulent vaginal discharge and cytological endometritis were greatest for MTnoC (91.7 and 91.4%), intermediate for MTC (74.0 and 73.3%), and smallest for NoMT (38.1 and 36.4%). Proportion of cyclic cows was smaller for MTnoC compared with MTC and NoMT (62.0, 71.0, and 71.0%). Pregnancy per artificial insemination following first service was smaller for cows with metritis compared with their counterparts with no metritis (NoMT = 28.1, MTC = 26.1, MTnoC = 22.0%). Pregnancy loss tended to be greater for MTnoC compared with MTC (NoMT = 11.5, MTC = 11.1, MTnoC = 18.4%). Hazard of pregnancy by 300 DIM was smallest for MTnoC, intermediate for MTC, and greatest for NoMT. Death by 60 DIM (3.9, 1.1, and 0.6%) and removal from herd by 300 DIM (26.3, 17.4, and 15.4%) were greatest for MTnoC compared with MTC and NoMT, respectively. Milk production among multiparous cows was smaller for MTnoC compared with MTC and NoMT in the first 10 mo postpartum, whereas MTC produced less milk compared with NoMT only during the first 2 mo postpartum (NoMT = 42.0 ± 0.22, MTC = 40.6 ± 0.28, MTnoC = 37.7 ± 0.54 kg/d). Failure of clinical cure was not associated with milk yield in primiparous cows (NoMT = 35.2 ± 0.31, MTC = 33.9 ± 0.31, MT-noC = 35.0 ± 0.52 kg/d). Cows diagnosed with metritis that do not undergo clinical cure by 10 d of onset of antimicrobial therapy have impaired reproductive performance, reduced milk production, and increased risk of leaving the herd.

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Uterine infusion of bacteria alters the transcriptome of bovine oocytes

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 9 publications

References 46 publications

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

Interrogating the bovine reproductive tract metagenomes using culture-independent approaches: a systematic review

Risk factors associated with testing positive for brucellosis and occurrence of abortion in high-yielding Holstein heifers

Failure of clinical cure in dairy cows treated for metritis is associated with reduced productive and reproductive performance

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