Uterine Rat Telocyte Structure and Organization: An Immunohistochemical and Ultrastructural Study

Abstract

Telocytes are a kind of interstitial cell found in a variety of organs, including the uterus. They regulate uterine contractions, maintain pregnancy, and prevent premature labour. The purpose of this research was to look at the organisation and distribution of telocytes in different uterine layers in adult and senile rats at various stages of reproduction. Materials & Methods: Twenty-four female rats were evenly divided between four groups: GI: adult non-pregnant, GII pregnant, GIII postpartum, and GIV senile. The uterine samples from middle one-third of the right horns were processed for light microscopic examination were treated using C-kit stains for immunohistochemical detection of telocytes, and TEM examination. Results: Telocytes were discovered in the endometrium and myometrium of adult non-pregnant uteri as tiny cells with numerous lengthy telopodes. Endometrial telocytes increased significantly, but myometrial telocytes decreased significantly in (GII). (GIII) and (GIV) had the largest count of myometrial telocytes. Conclusion: Telocytes were found in the endometrium and myometrium of the rat uterus. Telocytes serve as a uterine peacekeeper, initiating and coordinating myometrial contraction.

Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro

“…The endometrium is composed of a simple columnar epithelium situated above the glandular lamina propria, and the myometrium is composed of the inner circular, the middle stratum vascular (SV), and the outer longitudinal layers. Consistent with previous findings, IF results revealed the presence of CD34+/Vimentin+ TCs distributed across all layers of uterine tissue (Es, 2022). Co‐labeling with CD34/Vimentin+ showed that in the endometrium layer, TCs displayed large cellular bodies with long Tps extending around the glands and blood vessels, forming a 3D network structure through homocellular or heterocellular contacts to communication (Figure 1a,b).…”