Uterine, vaginal and placental blood flows increase with dynamic changes in serum metabolic parameters and oxidative stress across gestation in buffaloes

Elmetwally, Mohammed A; Elshopakey, Gehad E.; Eldomany, Wael; Eldesouky, Ashraf; Samy, Alaa; Lenis, Yasser Y.; Chen, Dong‐Bao

doi:10.1111/rda.13858

Cited by 11 publications

(10 citation statements)

References 28 publications

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“…Foetal blood demand increases gradually with pregnancy advancement being dramatic especially between mid to late gestation (Zaher et al, 2012). Uterine arteries' blood flow (UBF) controls placental functions and thickness and subsequently foetal well‐being (Elmetwally et al, 2021). Evaluation of uterine haemodynamics using Doppler ultrasonography has been considered a noninvasive, reliable and accurate tool, providing an insight into the placental and foetal status (EL‐Sherbiny, EL‐Shahat, et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Exogenous L‐arginine administration improves uterine vascular perfusion, uteroplacental thickness, steroid concentrations and nitric oxide levels in pregnant buffaloes under subtropical conditions

El‐Sherbiny

Samir

El-Shalofy

et al. 2022

Reprod Domestic Animals

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Heat stress (HS) during pregnancy adversely affects uterine vascular perfusion and foetal development. L‐arginine (L‐Arg), a nitric oxide (NO) precursor, has been proven to enhance an organ's vascular perfusion. Therefore, this study aimed to examine the effect of L‐Arg administration on the pregnant buffaloes' uterine haemodynamics and uteroplacental thickness under environmental HS conditions. For this purpose, pluriparous (n = 12) HS‐pregnant buffaloes (mid‐gestation, 180–190 days; temperature humidity index >85) were haphazardly assigned into two groups, either administered a single intravenous (IV) bolus of 5 mg/kg BW of L‐Arg Hcl (n = 6; ARG) or received normal saline (0.9%; IV; 25 ml) that served as a control group (n = 6; CON). Combined uteroplacental thickness (CUPT, mm), middle uterine arteries' (MUA) diameter (mm) and uterine Doppler haemodynamics (ipsilateral and contralateral to the pregnant horn) were examined utilizing B‐mode and colour Doppler ultrasonography, respectively. The serum levels of progesterone (P4), estradiol 17β (E2), nitric oxide (NO) and total antioxidant capacity (TAC) were assayed using commercial kits. The experimental time points were − 1, 0, 2, 4, 24, 48, 72, 96 and 120 h post‐L‐Arg administration. The ARG group showed higher (p < .05) CUPT and MUA diameter values starting at 24 h post‐treatment and onward than the control buffalo cows. In addition, there were improvements (p < .05) in the blood flow parameters in the ipsilateral MUA after L‐Arg treatment evidenced by lower values of pulsatility and resistive indices starting at 48 and 4 h, respectively and onward, and higher values of peak systolic velocity, coloured areas towards the pregnant uterine horn (both 24–72 h). Increases in the NO levels were found during the period between 4 and 120 h in the ARG group compared to the CON group. Moreover, significant increases in the E2 and P4 means were noted in the ARG group, especially at 24 h onward, respectively compared to the CON group. Concerning the TAC status, neither the administration nor the hours affected serum TAC levels in the HS pregnant buffaloes (CON and ARG). In conclusion, L‐Arg administration improved uteroplacental thickness and enhanced uterine haemodynamics, NO levels, and steroids production in mid‐pregnant buffalo cows under environmental heat stress conditions which could improve foetal growth and development.

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Section: Introductionmentioning

confidence: 99%

Exogenous L‐arginine administration improves uterine vascular perfusion, uteroplacental thickness, steroid concentrations and nitric oxide levels in pregnant buffaloes under subtropical conditions

El‐Sherbiny

Samir

El-Shalofy

et al. 2022

Reprod Domestic Animals

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“…In this study, changes in vaginal artery blood flow in dairy cows were investigated for the first time during the G:PG Ovsynch program. A previous study of pregnant buffalo characterized changes in vaginal blood flow during pregnancy [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…The time-averaged maximum velocity (TAMV), resistance index (RI), pulsatility index (PI), resistance impedance (S/D), peak velocity (PV) and blood flow volume (BFV), as well as the diameters of the uterine and vaginal arteries, were the Doppler indices that the device displayed for each waveform when using the automatic mode as previously reported [ 35 ]. B-mode images were used to measure the diameters of the uterine and vaginal arteries.…”

Section: Methodsmentioning

confidence: 99%

The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows

et al. 2022

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Aim OvSynch is a hormonal protocol for synchronization of estrus and use of artificial insemination (AI) at an optimal time without adverse effects on the ovaries or uterus. This study investigated the use of noninvasive color Doppler ultrasound to assess changes in uterine and vaginal blood flow during the Ovsynch program for synchronization of estrus and its relation to the pregnancy rates in Holstein cows. Materials and methods The experimental cows received an intramuscular dose of 10 μg of a GnRH analogue (G1), followed 7 days later with an intramuscular injection of synthetic prostaglandin F2α (P: PGF2α) analogue (500 μg cloprostenol sodium), and given a 10 μg, injection of the GnRH analogue (G2) i.m. 48 h after the PGF2α treatment, and the cows were bred 14-16 h after. Uterine and vaginal perfusion were investigated by performing transrectal Doppler ultrasonography of both the uterine and vaginal arteries in Holstein cows at different time points during the Ovsynch program to determine: peak systolic velocity (PSV), time-averaged maximum velocity (TAMV), the volume of blood flow (BFV), pulsatility index (PI), resistance index (RI), resistance impedance (S/D) and diameters of uterine (UA) and vaginal (VA) arteries. Steroid hormones were also assayed. Transrectal ultrasonography (TUS) was performed at 32 and 60 days to confirm the pregnancy per artificial insemination (P/AI). Results The uterine PSV, TAMV, and PV were greater at the time of the cloprostenol sodium and second GnRH injections ( p< 0.05) than at the time of the first GnRH injection. The vaginal PSV, PV were greater at the time of the cloprostenol sodium than at the time of the first and second GnRH injections ( p< 0.05). The receiver operating characteristic curve (ROC curve) indicated a high correlation between the uterine and vaginal blood flow and the rate of the pregnancy ( p< 0.05). The area under the ROC curve was 0.920 and 0.87 ( p< 0.05) for vaginal and uterine arteries respectively at time of G2. The serum levels of progesterone, estrogen and cortisol were correlated with the P/AI ( p< 0.05). The P/AI significantly decreased from 43.9 % at 32 d to 35.37 % at 60 d. Conclusion These results indicate that noninvasive Doppler ultrasonography is a valid method to evaluate changes in the characteristics of uterine and vaginal blood flow in cows during the Ovsynch protocol. Furthermore, vaginal and uterine blood flow are two determinant factors for the higher conception rates in Holstein dairy cows.

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“…The time-averaged maximum velocity (TAMV), resistance index (RI), pulsatility index (PI), resistance impedance (S/D), peak velocity (PV) and blood ow volume (BFV), as well as the diameters of the uterine and vaginal arteries, were the Doppler indices that the device displayed for each waveform when using the automatic mode as previously reported (31) . B-mode images were used to measure the diameters of the uterine and vaginal arteries.…”

Section: Concentrations Of Cortisol In Serummentioning

confidence: 97%

The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows

Sharawy

Hegab

Risha

et al. 2022

Preprint

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Aim OvSynch is a hormonal protocol for synchronization of estrus and use of artificial insemination (AI) at an optimal time without adverse effects on the ovaries or uterus. This study investigated the use of noninvasive color Doppler ultrasound to assess changes in uterine and vaginal blood flow during the Ovsynch program for synchronization of estrus and its relation to the pregnancy rates in Holstein cows. Materials and methods The experimental cows received an intramuscular dose of 10 µg of a GnRH analogue (G1), followed 7 days later with an intramuscular injection of synthetic prostaglandin F2α (P: PGF2α) analogue (500 µg cloprostenol sodium), and given a 10 µg, injection of the GnRH analogue (G2) i.m. (2.5ml/animal) 48 h after the PGF2a treatment, and the cows were bred 14–16 h after. Uterine and vaginal perfusion were investigated by performing transrectal Doppler ultrasonography of both the middle uterine and vaginal arteries in Holstein cows at different time points during the Ovsynch program to determine: peak systolic velocity (PSV), time-averaged maximum velocity (TAMV), the volume of blood flow (BFV), pulsatility index (PI), resistance index (RI), resistance impedance (S/D) and diameters of uterine (UA) and vaginal (VA) arteries. Steroid hormones were also considered. Transrectal ultrasonography (TUS) was performed at 32 and 60 days to confirm the pregnancy per artificial insemination (P/AI). Results The uterine PSV, TAMV, and PV were greater at the time of the cloprostenol sodium and second GnRH injections (p < 0.05) than at the time of the first GnRH injection. The vaginal PSV, PV were greater at the time of the cloprostenol sodium than at the time of the first and second GnRH injections (p < 0.05). The receiver operating characteristic curve (ROC curve) indicated a higher correlation between the uterine and vaginal blood flow and the rate of the pregnancy (p < 0.05). The ROC curve for the vaginal artery was 0.920 and that for uterine blood flow was 0.870 (p < 0.05) at the G2 (p < 0.05). The serum levels of progesterone, estrogen and cortisol were correlated with the P/AI (p < 0.05). The P/AI significantly decreased from 43.9% at 32 d to 35.37% at 60 d. Conclusion These results indicate that noninvasive Doppler ultrasonography is a valid method to evaluate changes in the characteristics of uterine and vaginal blood flow in cows during the Ovsynch protocol. Furthermore, vaginal and uterine blood flow are two determinant factors for the higher conception rates in Holstein dairy cows.

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Uterine, vaginal and placental blood flows increase with dynamic changes in serum metabolic parameters and oxidative stress across gestation in buffaloes

Cited by 11 publications

References 28 publications

Exogenous L‐arginine administration improves uterine vascular perfusion, uteroplacental thickness, steroid concentrations and nitric oxide levels in pregnant buffaloes under subtropical conditions

Exogenous L‐arginine administration improves uterine vascular perfusion, uteroplacental thickness, steroid concentrations and nitric oxide levels in pregnant buffaloes under subtropical conditions

The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows

The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows

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