Uterosome-like vesicles prompt human sperm fertilizing capability

Franchi, A.; Cubilla, Marisa A.; Guidobaldi, Héctor Alejandro; Bravo, Alejandra; Giojalas, Laura Cecilia

doi:10.1093/molehr/gaw066

Cited by 20 publications

(22 citation statements)

References 49 publications

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“…interactions between EVs of uterine or oviductal origin and spermatozoa may occur in a short time interval: even a 15-min incubation of spermatozoa with fluorescent labeled uterine EVs was sufficient to retrieve sperm with fluorescent staining, indicating the rapid fusion of EVs with sperm membrane (Franchi et al 2016). Thus, it may be that some of the sperm-interacting proteins identified in the present study were delivered to sperm membrane after fusion with oviductal EVs.…”

Section: Figurementioning

confidence: 66%

Identification by proteomics of oviductal sperm-interacting proteins

Lamy¹,

Nogues²,

Combes‐Soia³

et al. 2018

Reproduction

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The interactions between oviductal fluid (OF) proteins and spermatozoa play major roles in sperm selection, storage and capacitation before fertilization. However, only a few sperm-interacting proteins in the OF has been identified and very little is known about the regulation of sperm-oviduct interactions across the estrous cycle. Samples of bovine frozen-thawed sperm from three bulls were incubated with OF at pre-, post-ovulatory stages (Pre-/Post-ov) or luteal phase (LP) of the estrous cycle (7 mg/mL proteins, treated groups) or with a protein-free media (control). The proteomes of sperm cells were assessed by nanoLC-MS/MS and quantified by label-free methods. A total of 27 sperm-interacting proteins originating in the OF were identified. Among those, 14 were detected at all stages, eight at Post-ov and LP and five only at LP. The sperm-interacting proteins detected at all stages or at LP and Post-ov were on average more abundant at LP than at other stages ( < 0.05). At Pre-ov, OVGP1 was the most abundant sperm-interacting protein while at Post-ov, ACTB, HSP27, MYH9, MYH14 and OVGP1 were predominant. Different patterns of abundance of sperm-interacting proteins related to the stage were evidenced, which greatly differed from those previously reported in the bovine OF. In conclusion, this study highlights the important regulations of sperm-oviduct interactions across the estrous cycle and provides new protein candidates that may modulate sperm functions.

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Section: Figurementioning

confidence: 66%

Identification by proteomics of oviductal sperm-interacting proteins

Lamy¹,

Nogues²,

Combes‐Soia³

et al. 2018

Reproduction

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“…As we previously observed that uterosome‐like vesicles increase the human sperm iAR and PY, we evaluated the effect of oviductal EV on these capacitation parameters. The interaction of spermatozoa with EV from the isthmus or ampulla region increases 2.5‐fold the iAR, as similarly observed by uterosome‐like vesicles in human spermatozoa, and by vaginosomes after a brief interaction with murine spermatozoa prompted by progesterone …”

Section: Discussionmentioning

confidence: 99%

“…After seminal plasma removal, sperm suspensions were incubated for 90 minutes under different conditions: Non‐Cap, “non‐capacitating” medium (Sp‐TALP formulated as described above); Non‐Cap medium containing 10 µg/mL of EV (from isthmus or ampulla) or “capacitating” medium as control of capacitating conditions (Sp‐TALP supplemented with 25 mM NaHCO 3 and 3 mg/mL BSA; Sigma; Cat #A‐7906). The EV concentration was selected based on data from previous results and referred to the protein concentration.…”

Section: Methodsmentioning

confidence: 99%

Extracellular vesicles from oviductal isthmus and ampulla stimulate the induced acrosome reaction and signaling events associated with capacitation in bovine spermatozoa

Franchi

Moreno-Irusta

Domïnguez

et al. 2019

J of Cellular Biochemistry

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Cells can communicate with other neighboring or distant cells through the secretion of extracellular vesicles (EV), composed of a lipid bilayer and bearing surface molecules that allow them to recognize target cells. In this way, EV induce signaling via different mechanisms, modulating the physiological state of the recipient cell. EV have been identified in both male and female reproductive fluids, however, the possible role of EV isolated from female reproductive fluids has become an emerging field only recently. It is known that ejaculated mammalian spermatozoa need to undergo physiological preparation in the female reproductive tract to fertilize the egg. EV secreted by different regions of the female tract constitute signals that may have a key role in regulating sperm functions. The aims of the present study were isolating EV from different regions of the bovine oviduct and analyzing their interaction and physiological effects on spermatozoa. Here, we report the characterization of bovine oviductal fluid EV from the isthmus and ampulla region and their effect on the induced acrosome reaction and signaling events associated with sperm capacitation. EV induced an increase in sperm protein tyrosine phosphorylation, while cell survival of cryopreserved bovine spermatozoa was maintained. We also show that EV uptake regulates the sperm calcium levels by inducing an immediate increase in the intracellular calcium concentration and sperm priming, after a pre‐incubation period, of the progesterone‐induced intracellular calcium rise. Our data contribute to understand the role of EV in the communication between the female reproductive tract and the sperm physiology, information that may be used to improve the efficiency of reproductive assisted technologies.

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“…After leaving the vagina sperm enter the uterus. In vitro studies have shown that a short exposure (15 min) of sperm with uterosomes secreted by endometrial epithelial cells simulating the time that they spend in the uterus is enough to enhance sperm capacitation status in human spermatozoa [26]. Others authors have described the same results but with longer exposure times [21].…”

Section: Relationship Between Spermatozoa and Evs As A Tool To Enhancmentioning

confidence: 96%

Extracellular Vesicles, the Road toward the Improvement of ART Outcomes

Gervasi

Soler²,

González‐Fernández

et al. 2020

Animals

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Nowadays, farm animal industries use assisted reproductive technologies (ART) as a tool to manage herds’ reproductive outcomes, for a fast dissemination of genetic improvement as well as to bypass subfertility issues. ART comprise at least one of the following procedures: collection and handling of oocytes, sperm, and embryos in in vitro conditions. Therefore, in these conditions, the interaction with the oviductal environment of gametes and early embryos during fertilization and the first stages of embryo development is lost. As a result, embryos obtained in in vitro fertilization (IVF) have less quality in comparison with those obtained in vivo, and have lower chances to implant and develop into viable offspring. In addition, media currently used for IVF are very similar to those empirically developed more than five decades ago. Recently, the importance of extracellular vesicles (EVs) in the fertility process has flourished. EVs are recognized as effective intercellular vehicles for communication as they deliver their cargo of proteins, lipids, and genetic material. Thus, during their transit through the female reproductive tract both gametes, oocyte and spermatozoa (that previously encountered EVs produced by male reproductive tract) interact with EVs produced by the female reproductive tract, passing them important information that contributes to a successful fertilization and embryo development. This fact highlights that the reproductive tract EVs cargo has an important role in reproductive events, which is missing in current ART media. This review aims to recapitulate recent advances in EVs functions on the fertilization process, highlighting the latest proposals with an applied approach to enhance ART outcome through EV utilization as an additive to the media of current ART procedures.

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Uterosome-like vesicles prompt human sperm fertilizing capability

Abstract: The project was financially supported by SECyT-UNC. The authors declare no conflict of interest.

Cited by 20 publications

References 49 publications

Identification by proteomics of oviductal sperm-interacting proteins

Identification by proteomics of oviductal sperm-interacting proteins

Extracellular vesicles from oviductal isthmus and ampulla stimulate the induced acrosome reaction and signaling events associated with capacitation in bovine spermatozoa

Extracellular Vesicles, the Road toward the Improvement of ART Outcomes

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