Uterosomes Exosomal cargo during the estrus cycle and interaction with sperm

Martin‐DeLeon, Patricia A.

doi:10.2741/s451

Cited by 25 publications

(19 citation statements)

References 41 publications

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“…Given the potential importance of such EVs in the establishment of pregnancy, current knowledge of EV heterogeneity and function and their potential influence within the microenvironment of implantation is reviewed below. It is also likely that these EVs identified and characterized in the uterine cavity are the same as the uterosomes that have been studied for their uptake and effects on sperm (in mice) (summarized in [23]).…”

Section: Establishment Of Pregnancy: the Importance Of The Uterine MImentioning

confidence: 99%

Extracellular Vesicles in the Intrauterine Environment: Challenges and Potential Functions

Nguyen

Simpson

Salamonsen

et al. 2016

Biology of Reproduction

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Extracellular vesicles (EVs), including exosomes (30–150 nm) and microvesicles (100–1500 nm), play important roles in mediating cell-cell communication. Such particles package distinct cargo elements, including lipids, proteins, mRNAs, microRNAs, and DNA, that vary depending on the cell of origin and its phenotype. This cargo can be horizontally transferred to target cells where its components can reprogram the recipient cell to modify its function. EVs have been identified within the uterine cavity of women, sheep, and mice, where they contribute to the microenvironment of sperm transport, and of blastocyst and endometrial preparation for implantation. It is likely that exosomes and microvesicles carry different cargo and coordinate different roles in this intrauterine environment. Understanding and defining these subtypes of EVs is important for future functional studies and clinical translation. Here we critically review the various purification and validation procedures for extracellular vesicle analysis and discuss what is known of endometrial-derived exosome cargo and of their hormonal regulation. The current knowledge of the functions of uterine exosomes, with respect to sperm transport and function, and of their actions on trophectodermal cells to promote implantation are summarized and evaluated in their physiological context. Given the potential importance of this form of cell-cell interactions within the reproductive tract, the critical issues discussed will guide new insights in this rapidly expanding field.

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Section: Establishment Of Pregnancy: the Importance Of The Uterine MImentioning

confidence: 99%

Extracellular Vesicles in the Intrauterine Environment: Challenges and Potential Functions

Nguyen

Simpson

Salamonsen

et al. 2016

Biology of Reproduction

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“…On the one hand, EV from epididymis and prostate (epididymosomes and prostasomes, respectively) are well‐characterized and their roles in sperm physiology have been explored . On the other hand, EV isolated from female reproductive fluids have been recently reported to modulate sperm functions relevant for fertilization, a finding that positions the research as an emerging field.…”

Section: Introductionmentioning

confidence: 99%

“…3,4 On the one hand, EV from epididymis and prostate (epididymosomes and prostasomes, respectively) are well-characterized and their roles in sperm physiology have been explored. [5][6][7][8] On the other hand, EV isolated from female reproductive fluids have been recently reported [9][10][11] to modulate sperm functions relevant for fertilization, a finding that positions the research as an emerging field. Ejaculated mammalian spermatozoa are not able to fertilize an oocyte after they leave the male reproductive tract; instead, they must remain in the female reproductive tract for a period to achieve fertilizing ability.…”

mentioning

confidence: 99%

Extracellular vesicles from oviductal isthmus and ampulla stimulate the induced acrosome reaction and signaling events associated with capacitation in bovine spermatozoa

Franchi

Moreno-Irusta

Domïnguez

et al. 2019

J of Cellular Biochemistry

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Cells can communicate with other neighboring or distant cells through the secretion of extracellular vesicles (EV), composed of a lipid bilayer and bearing surface molecules that allow them to recognize target cells. In this way, EV induce signaling via different mechanisms, modulating the physiological state of the recipient cell. EV have been identified in both male and female reproductive fluids, however, the possible role of EV isolated from female reproductive fluids has become an emerging field only recently. It is known that ejaculated mammalian spermatozoa need to undergo physiological preparation in the female reproductive tract to fertilize the egg. EV secreted by different regions of the female tract constitute signals that may have a key role in regulating sperm functions. The aims of the present study were isolating EV from different regions of the bovine oviduct and analyzing their interaction and physiological effects on spermatozoa. Here, we report the characterization of bovine oviductal fluid EV from the isthmus and ampulla region and their effect on the induced acrosome reaction and signaling events associated with sperm capacitation. EV induced an increase in sperm protein tyrosine phosphorylation, while cell survival of cryopreserved bovine spermatozoa was maintained. We also show that EV uptake regulates the sperm calcium levels by inducing an immediate increase in the intracellular calcium concentration and sperm priming, after a pre‐incubation period, of the progesterone‐induced intracellular calcium rise. Our data contribute to understand the role of EV in the communication between the female reproductive tract and the sperm physiology, information that may be used to improve the efficiency of reproductive assisted technologies.

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“…Significantly, soma‐to‐gamete transfer via sperm is not exclusive to male donors. Somatic EV‐mediated protein transfer to sperm from the mother also occurs prefertilization in the mouse uterus …”

Section: Crossing Cellular Taxonomic and Weismann Barriers: Extracementioning

confidence: 99%

“…Somatic EV-mediated protein transfer to sperm from the mother also occurs prefertilization in the mouse uterus. 147 The effects of extracellular DNA and EV transmission to spermatozoa are not necessarily limited to the gametes or zygotes. bbb The soma-to-germ line EV transfers influence epigenetic programming and development of next-generation embryos.…”

Section: Ev Communication Between Somatic and Germline Cells In Sexuamentioning

confidence: 99%

No genome is an island: toward a 21st century agenda for evolution

Shapiro

2019

Annals of the New York Academy of Sciences

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Conventional 20th century evolution thinking was based on the idea of isolated genomes for each species. Any possibility of life‐history inputs to the germ line was strictly excluded by Weismann's doctrine, and genome change was attributed to random copying errors. Today, we know that many life‐history events lead to rapid and nonrandom evolutionary change mediated by specific cellular functions. There are many ways that genomes, viruses, cells, and organisms interact to generate evolutionary variation. These include cell mergers and activation of natural genetic engineering by stress, infection, and interspecific hybridization. In addition, we know molecular mechanisms for transmitting life‐history information across generations through gametes. These discoveries require a new agenda for evolutionary theory and novel experimental designs to investigate the genomic impacts of stresses, biotic interactions, and sensory inputs coming from the environment. The review will offer some generic recommendations for enriching evolution experiments to incorporate new knowledge and find answers to previously excluded questions.

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Uterosomes Exosomal cargo during the estrus cycle and interaction with sperm

Cited by 25 publications

References 41 publications

Extracellular Vesicles in the Intrauterine Environment: Challenges and Potential Functions

Extracellular Vesicles in the Intrauterine Environment: Challenges and Potential Functions

Extracellular vesicles from oviductal isthmus and ampulla stimulate the induced acrosome reaction and signaling events associated with capacitation in bovine spermatozoa

No genome is an island: toward a 21st century agenda for evolution

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