Uterotropic effects of dietary equol administration in ovariectomized Sprague–Dawley rats

Rachoń, Dominik; Vortherms, Tina; Seidlová-Wuttke, Dana; Menche, Anne; Wuttke, W.

doi:10.1080/13697130701624757

Cited by 33 publications

(20 citation statements)

References 51 publications

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“…Our current weight gain data support those of others showing an attenuation of BW following E 2 supplementation in OVX rats (Cooke & Naaz 2004, Rachon et al 2007a. However, no such effects were observed with SPI feeding.…”

Section: Discussionsupporting

confidence: 81%

“…Thus, current findings and our previous data demonstrate that SPI feeding does not elicit classical estrogenic responses on ERa-responsive tissues such as the uterus, even when utilized as the sole protein source. By contrast, mature OVX rats fed purified equol (400 mg/kg) or injected with purified genistein (54 mg/kg) displayed mild estrogen-like uterine stimulation (Rachon et al 2007a, Rimoldi et al 2007. Studies utilizing purified soy isoflavones were shown to regulate gene expression similar to estrogens in female (Naciff et al 2002) and male reproductive tissues (Naciff et al 2004(Naciff et al , 2005.…”

Section: Discussionmentioning

confidence: 99%

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Hepatic gene expression following consumption of soy protein isolate in female Sprague–Dawley rats differs from that produced by 17β-estradiol treatment

Singhal¹,

Shankar²,

Badger³

et al. 2009

Journal of Endocrinology

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Although soy foods have been recognized as an excellent source of protein, there have been recent concerns regarding potential adverse effects of isoflavone phytochemicals found in soy products, which are known to bind and activate estrogen receptors. Here, we used global hepatic gene expression profiles in ovariectomized female SpragueDawley rats treated with 17b-estradiol (E 2 ) or fed with soy protein isolate (SPI) as a means of estimating potential estrogenicity of SPI. Female Sprague-Dawley rats were fed AIN-93G diets containing casein (CAS) or SPI starting at postnatal day (PND) 30. Rats were ovariectomized on PND 50 and infused with E 2 or vehicle in osmotic pumps for 14 d. Microarray analysis was performed on liver using Affymetrix GeneChip Rat 230 2.0. Serum E 2 levels were within normal ranges for the rat and SPI feeding did not increase uterine wet weight in the absence or presence of E 2 . SPI feeding altered (P!0 . 05, RG1 . 5-fold) the expression of 82 genes, while E 2 treatment altered 892 genes. Moreover, only 4% of E 2 -affected genes were also modulated by SPI, including some whose expression was reversed by SPI feeding. The interaction between E 2 and SPI uniquely modulated the expression profile of 225 genes including the reduction of those involved in fatty acid biosynthesis or glucocorticoid signaling and an induction of those involved in cholesterol metabolism. The different hepatic gene signatures produced by SPI feeding compared with E 2 and the lack of increase in uterine wet weight in rats fed with SPI suggest that SPI is not estrogenic in these tissues.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Hepatic gene expression following consumption of soy protein isolate in female Sprague–Dawley rats differs from that produced by 17β-estradiol treatment

Singhal¹,

Shankar²,

Badger³

et al. 2009

Journal of Endocrinology

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“…Rats were orally gavaged for 5 consecutive days with olive oil (1 ml/rat/day) as the vehicle control group, equol low dose (100 mg/kg BW/day), equol high dose (250 mg/kg BW/day), and flutamide (100 mg/kg BW/day) as the anti-androgenic reference positive control group. The low dose equol was determined due to it being the level at which estrogenic effects were observed in ovariectomized rats, but that did not result in negative toxicity [31,32], and was also equivalent to the effective dose of flutamide. The high dose equol was chosen to investigate if the possible androgen antagonistic effects could be detected with a pharmacologically relevant dose.…”

Section: Experimental Designmentioning

confidence: 99%

The influence of equol on the hypothalamic–pituitary–thyroid axis and hepatic lipid metabolic parameters in adult male rats

2015

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“…Cross-sectional analysis showed an improvement in bone mineral density for postmenopausal, but not premenopausal women. Rachoń et al (2007b) demonstrated a biphasic effect; dietary EQO administration had no effect in the proximal tibial metaphysis, while an enhancement was seen in the lumbar spine of rats. However, according to Setchell and Cole's theory (2006), the clinical effectiveness of soy protein may be a function of its ability to transform soy isofl avones to the more potent EQO.…”

Section: Discussionmentioning

confidence: 89%

Effects of isoflavones equol and genistein on bone quality in a rat osteopenia model

Sehmisch

Erren

Kolios

et al. 2010

Phytotherapy Research

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Phytoestrogens might be an alternative medication in prophylaxis and treatment of osteoporosis. In this study, the osteoprotective effects of genistein (GEN) and equol (EQO) were evaluated. After ovariectomy, 44 rats received soy-free food (Control, C) and developed substantial osteoporosis over the course of two months. After that period, the rats were divided into different groups and fed estradiol (E), GEN or EQO for 35 days. To analyze the osteoprotective effects of the tested substances, bone biomechanical properties and histomorphometric changes of the lumbar vertebrae were evaluated. In analyzing the vertebral body compression strength, we found that the EQO (103.8%) and GEN (96.8%) groups reached similar levels relative to the E group, while the C group reached 77.7% of the biomechanical properties of the E group. EQO was significantly superior to C. The histomorphometric evaluation demonstrated an increased number of nodes in EQO- and E-treated rats compared to GEN- and C-treated rats. E led to an improvement of cortical as well as trabecular bone, an advantage that was only partly seen in the other groups. Treatment with phytoestrogens induced improved bone quality. EQO and GEN might be alternatives for hormone replacement therapy, although further studies are needed to elucidate possible side effects.

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Uterotropic effects of dietary equol administration in ovariectomized Sprague–Dawley rats

Abstract: Long-term high-dose dietary equol administration to ovariectomized rats exerts uterotropic effects at the cellular and molecular level which question the safety of uncontrolled and unlimited consumption of soy or red clover supplements by postmenopausal women with intact uteri.

Cited by 33 publications

References 51 publications

Hepatic gene expression following consumption of soy protein isolate in female Sprague–Dawley rats differs from that produced by 17β-estradiol treatment

Hepatic gene expression following consumption of soy protein isolate in female Sprague–Dawley rats differs from that produced by 17β-estradiol treatment

The influence of equol on the hypothalamic–pituitary–thyroid axis and hepatic lipid metabolic parameters in adult male rats

Effects of isoflavones equol and genistein on bone quality in a rat osteopenia model

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