UTEX—THE CULTURE COLLECTION OF ALGAE AT THE UNIVERSITY OF TEXAS AT AUSTIN 1993 LIST OF CULTURES<sup>1</sup>

Starr, Richard C.; Zeikus, Jeffrey A.

doi:10.1111/j.0022-3646.1993.00001.x

Cited by 785 publications

(562 citation statements)

References 396 publications

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“…1) were cultured as unialgal isolates in autoclaved seawater with a half-strength Provasoli enrichment (Starr & Zeikus, 1987). Stock cultures were maintained at 25°C, under long day (14: 10hLD) conditions at a photon fluence rate of 5-20 #mol m -2 s -~…”

Section: Methodsmentioning

confidence: 99%

Temperature responses of tropical to warm-temperate Atlantic seaweeds. I. Absence of ecotypic differentiation in amphi-Atlantic tropical-Canary Islands species

Pakker

Breeman

Reine

et al. 1996

European Journal of Phycology

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Section: Methodsmentioning

confidence: 99%

Temperature responses of tropical to warm-temperate Atlantic seaweeds. I. Absence of ecotypic differentiation in amphi-Atlantic tropical-Canary Islands species

Pakker

Breeman

Reine

et al. 1996

European Journal of Phycology

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“…Female and male gametophyte clones germinated from zoospores were isolated according to cell size (about 20 μm for females and 10 μm for males) under a microscope and cultured in 500-mL flasks under vegetative growth conditions of 30 μmol photons m −2 s −1 at 17 ± 1°C, with a photoperiod of 12 h: 12 h light: dark as previously described (Zhou and Wu 1998). During culture, the flasks were shaken several times by hand at intervals each day, and the Provasoli's enriched seawater (PES) medium (Starr and Zeikus 1993) was replaced every 2 weeks.…”

Section: Algal Strain and Culturementioning

confidence: 99%

Characterization and physical mapping of nuclear ribosomal RNA (rRNA) genes in the haploid gametophytes of Saccharina japonica (Phaeophyta)

et al. 2017

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Nuclear tandemly repeated ribosomal RNA genes (18S-5.8S-25S rDNA and 5S rDNA) have been proven to be excellent cytogenetic markers for karyotype analysis of various higher plants by using the fluorescence in situ hybridization (FISH) technique. To illustrate physical mapping of these rDNAs in brown seaweed, Saccharina japonica, chromosomes, an approximately 5400-bp transcription unit of 18S-5.8S-25S rDNA was assembled by cloning the 25S rDNA after paired-end sequencing of two screened clones from a bacterial artificial chromosome library of kelp gametophytes. In contrast to the conserved 18S-5.8S-25S rDNA and ITS1 in S. japonica, the 245-bp ITS2 was variable in sequence. The cloned 5S rDNA revealed that the 120-bp conserved coding region was separated by a diverse intergenic spacer sequence that were 250, 445, 905, or 1335 bp in length. On average, the 18S-5.8S-25S rDNA of kelp female and male gametophytes had 45 and 41 copies per haploid genome, respectively, as detected by quantitative real-time PCR, whereas the 5S rDNA had 2590 and 2648 copies, respectively. Southern hybridization with labeled probes of 18S rDNA or 5S rDNA demonstrated that kelp gametophytes possessed only one locus each of the 18S-5.8S-25S or 5S rDNAs. This was further confirmed by FISH analysis using the same labeled probes, thus illustrating that 18S-5.8S-25S rDNA is located at the intercalary region of chromosome 23, whereas 5S rDNA at the sub-telomeric region of chromosomes 27. The localization of these rDNAs using the FISH technique has facilitated the identification of individual chromosomes and karyotype analysis of this kelp.

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“…Isochrysis aff. galbana (UTEX 2307) was cultivated semicontinuously in artificial seawater (Starr & Zeikus 1993); for Chaetoceros calcitrans (IFremer), diluted seawater medium (50%) was used. Cells were concentrated by centrifugation.…”

Section: (B) Preparation Of Foodmentioning

confidence: 99%

Strong influences of larval diet history on subsequent post–settlement growth in the freshwater molluscDreissena polymorpha

Wacker

Elert

2002

Proc. R. Soc. Lond. B

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A significant seasonal variation in size at settlement has been observed in newly settled larvae of Dreissena polymorpha in Lake Constance. Diet quality, which varies temporally and spatially in freshwater habitats, has been suggested as a significant factor influencing the life history and development of freshwater invertebrates. Accordingly, experiments were conducted with field-collected larvae to test the proposal that diet quality can determine planktonic larval growth rates, size at settlement and subsequent post-metamorphic growth rates. Larvae were fed one of two diets or starved. One diet was composed of cyanobacterial cells, which are deficient in polyunsaturated fatty acids (PUFAs) and the other was a mixed diet rich in PUFAs. Freshly metamorphosed animals from the starvation treatment had a carbon content per individual 70% lower than that of larvae fed the mixed diet. This apparent exhaustion of larval internal reserves resulted in a 50% reduction of the post-metamorphic growth rates. Growth was also reduced in animals previously fed the cyanobacterial diet. Hence, low food quantity or low food quality during the larval stage of D. polymorpha, lead to irreversible effects for post-metamorphic animals and are related to inferior competitive abilities.

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UTEX—THE CULTURE COLLECTION OF ALGAE AT THE UNIVERSITY OF TEXAS AT AUSTIN 1993 LIST OF CULTURES¹

Cited by 785 publications

References 396 publications

Temperature responses of tropical to warm-temperate Atlantic seaweeds. I. Absence of ecotypic differentiation in amphi-Atlantic tropical-Canary Islands species

Temperature responses of tropical to warm-temperate Atlantic seaweeds. I. Absence of ecotypic differentiation in amphi-Atlantic tropical-Canary Islands species

Characterization and physical mapping of nuclear ribosomal RNA (rRNA) genes in the haploid gametophytes of Saccharina japonica (Phaeophyta)

Strong influences of larval diet history on subsequent post–settlement growth in the freshwater molluscDreissena polymorpha

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UTEX—THE CULTURE COLLECTION OF ALGAE AT THE UNIVERSITY OF TEXAS AT AUSTIN 1993 LIST OF CULTURES1

Cited by 785 publications

References 396 publications

Temperature responses of tropical to warm-temperate Atlantic seaweeds. I. Absence of ecotypic differentiation in amphi-Atlantic tropical-Canary Islands species

Temperature responses of tropical to warm-temperate Atlantic seaweeds. I. Absence of ecotypic differentiation in amphi-Atlantic tropical-Canary Islands species

Characterization and physical mapping of nuclear ribosomal RNA (rRNA) genes in the haploid gametophytes of Saccharina japonica (Phaeophyta)

Strong influences of larval diet history on subsequent post–settlement growth in the freshwater molluscDreissena polymorpha

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UTEX—THE CULTURE COLLECTION OF ALGAE AT THE UNIVERSITY OF TEXAS AT AUSTIN 1993 LIST OF CULTURES¹